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S A Risin

Publications and source records attributed to S A Risin.

7 recordsLinked to original sources
PubMedDate not supplied

[The nature of interactions between total histones and membranes of isolated lysosomes].

Total fraction of histones increased the permeability of isolated lysosomes as showed the elevation in nonsedimented and free enzymatic activity of the matrix. S-like shape of curves, observed in dose-dependent effect of the histones increased concentrations on the enzymatic activity, indicated the structure transformations of membranes occurring by the cooperative type. Membranotropic effect of histones exceeded the action on non-ionic detergent Triton X-100. Hydrophobic interactions of histones with lysosomal membranes appear to be responsible for the effects observed.

Acid Phosphatase↗
PubMedDate not supplied

[Effect of histones on mouse liver mitochondria cytochrome oxidase activity in vitro and in vivo].

Histones at concentration of 2-10 micrograms/ml activated the mitochondrial cytochrome oxidase and at concentration of 25 micrograms/ml and higher--inhibited the enzyme in vitro. Cytochrome oxidase was completely inactivated by histones at concentration of 100 mg/microliter and higher. After administration in vivo histones modified the reaction of liver mitochondria in response to the subsequent treatment with a non-ion detergent Triton X-100. Possible pathogenetic importance of the phenomena observed is discussed.

Animals↗
PubMedDate not supplied

[Effect of unfractionated histones on isolated mouse liver lysosomes].

Total histones increased the permeability of lysosomal membranes for enzymes of matrix in the preparation of isolated lysosomes, where free and nonsedimented activity of acid phosphatase was estimated. The phenomenon depended on concentration of histones in a mixture. Histones apparently destructed the lysosomal membranes at concentration of 100 mg/ml and higher, since the level of free and nonsedimented activity of acid phosphatase approximated and even exceeded the enzymatic activity after treatment of the lysosomes with 0.2% Triton X-100. These properties of histones depended on their macromolecular structure. Histones did not affect the activity of the solubilized enzyme.

Acid Phosphatase↗
PubMedDate not supplied

Fluorescent in situ hybridization analysis in blood lymphocytes of lung cancer patients.

Genetic predisposition to lung cancer was determined by observing nonrandom chromosomal alterations in peripheral blood lymphocytes (PBLs) of lung cancer patients. The histological distribution of the cases showed that chromosomes 7 and 9 were frequently altered in squamous cell lung carcinoma (SCLC) patients. We analyzed PBLs of 26 SCLC patients and 5 controls using fluorescent in situ hybridization (FISH) with whole chromosome painting probes of chromosomes 7 and 9 to further investigate the frequency of rearrangements in these chromosomes. Our results suggested that seeking nonrandom aberrations in larger numbers of cells using FISH strengthened our previous observation of mosaicism and involvement of specific chromosomes in lung cancer patients. On combining our previous data, aberrations in chromosome 7 (16 of 26 patients), chromosome 9 (14 of 26), and the present study, we could actually pinpoint more individuals with abnormalities of chromosome 7 (23 of 26) and chromosome 9 (21 of 26). Thus, analyzing more cells in PBLs and adding FISH analysis serve as useful adjuncts to our studies of nonrandom chromosomal aberrations and genetic mosaicicm.

Adult↗