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S B Debast

Publications and source records attributed to S B Debast.

7 recordsLinked to original sources
PubMed2005

[Clostridium difficile ribotype 027, toxinotype III in The Netherlands].

Recently, two Dutch hospitals reported outbreaks of Clostridium difficile ribotype 027, toxinotype III. This strain, which was seen earlier in the United States, Canada and the United Kingdom, produces large amounts of toxins due to a defect in the toxin-regulating gene and causes severe diarrhoea. Antibiotic use, especially use of fluoroquinolones, is a risk factor. Control of outbreaks is hampered by the fact that Clostridium forms spores that can survive for a very long time in the environment and are resistant to the usual surface disinfectants. Protocols for diagnostic investigations, prevention and control of outbreaks are available.

Bacterial Toxins↗
PubMed1996

Use of interrepeat PCR fingerprinting to investigate an Acinetobacter baumannii outbreak in an intensive care unit.

An epidemiological investigation of an outbreak of Acinetobacter baumannii among patients on 2 closely related intensive care units (ICU) was performed by molecular typing with interrepeat polymerase chain reaction (interrepeat PCR). 31 A. baumannii isolates obtained from 15 ICU patients were characterized. All patients were infected or colonized with A. baumannii. After identification of the outbreak, 6 environmental isolates were collected from tap-water, sinks and cleaning detergents. PCR fingerprinting identified 3 genotypes among the outbreak-related strains. One predominant genotype was demonstrated in 14/15 patients and this genotype was also found among all environmental isolates. The cluster of A. baumannii represented an outbreak of 1 genotype, suggesting cross-contamination. The finding of the identical genotype among all environmental strains indicated a common environmental source causing the outbreak. The outbreak was controlled after reimplementation of an effective disinfection of workplace surfaces. This survey proved interrepeat PCR to be a rapid and reliable method to differentiate A. baumannii strains, thereby allowing epidemiological surveillance of large amounts of strains and early interventions to control outbreaks.

Acinetobacter↗
PubMedDate not supplied

Epidemiological survey of an outbreak of multiresistant Serratia marcescens by PCR-fingerprinting.

During an outbreak of Serratia marcescens from May to November 1993 43 strains obtained from 27 ICU patients infected or colonized with multiresistant S. marcescens were genotypically characterized with random amplified polymerase chain reaction (RAPD-PCR)-fingerprinting. In addition, 43 epidemiologically unrelated control isolates were selected. PCR-fingerprinting identified ten different genotypes of S. marcescens among the outbreak related strains. One predominant genotype was demonstrated in 21/43 isolates of 11/27 patients. A cluster of this genotype was found in seven/eight patients on the cardiosurgical ICU. The epidemiologically unrelated strains all showed different genotypes as compared to the predominant type. This survey proved RAPD-PCR to be a highly discriminatory and reproducible method for epidemiological studies of S. marcescens strains in nosocomial outbreaks.

Bacterial Typing Techniques↗
PubMedDate not supplied

The effects of a tactile stimulation/range-finding programme on the development of very low birth weight infants during initial hospitalization.

During 1984 and 1985, after their referral to the intermediate care nursery, 51 healthy very low birth weight infants were subjected to a tactile stimulation programme imitating the spatial limitation which occurs towards the end of gestation. The sensory motor development of the study group was compared with the development of a control group during initial hospitalization. The study and control group differed significantly in the neonatal period after completion of the programme. The study group showed better auditive responses (P less than 0.02), more variations in hand movements (P less than 0.01), less hypotony (P less than 0.02), more sucking (P less than 0.02), and less bradycardia and apnoea (P less than 0.01).

Arousal↗