Improved immunoperoxidase staining on semi-thin sections of epoxy-embedded tissues.
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Biomedical subjects
Publications and source records attributed to S B Or.
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A rapid method for re-embedding paraffin sections into epoxy resin for diagnostic electron microscopy is described. The method requires a relatively shorter time than the traditional block retrieval technique and produces a reasonable retrieval technique and produces a reasonable quality of ultrastructure for diagnostic purposes.
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A preparation method for x-ray analysis of inorganic deposits on paraffin sections is described. It involves the use of resin-slides for mounting paraffin sections which are then dewaxed, carboncoated and examined in the SEM. The resin support, being much cheaper than the carbon planchet and having a perfectly flat surface, facilitates the adhesion of paraffin sections to it, and slides can be stored desiccated for retrospective study. The method also permits direct examination of sections under a light microscope to locate areas of interest. It is simple and useful for the analysis of inorganic inclusions in biological samples.