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Biomedical subjects

S B Reed

Publications and source records attributed to S B Reed.

At least 19 recordsLinked to original sources
PubMed2001

Molecular characterization of a novel Staphylococcus aureus serine protease operon.

The present study identified and characterized a unique operon (spl) encoding six serine protease-like proteins. In addition, native Spl proteins were isolated and characterized. Typical of most exoproteins, the spl gene products contain putative 35- or 36-amino-acid signal peptides. The Spl proteins share 44 to 95% amino acid sequence identity with each other and 33 to 36% sequence identity with V8 protease. They also contain amino acids found in catalytic triads of enzymes in the trypsin-like serine protease family, and SplB and SplC were shown to degrade casein. The spl operon is transcribed on a 5.5-kb transcript, but several nonrandom degradation products of this transcript were also identified. Similar to other S. aureus exoprotein genes, the spl operon is maximally expressed during the transition into stationary phase and is positively controlled by the Agr virulence factor regulator. The Sar regulatory system did not affect spl operon expression. PCR analysis revealed the presence of the spl operon in 64% of the S. aureus isolates tested, although one spl operon-negative isolate was shown to contain at least two of the spl genes. Finally, intraperitoneal injection of an spl operon deletion mutant revealed no major differences in virulence compared to the parental strain.

Amino Acid Sequence↗
PubMed1996

Reporting biases in hypnosis: suggestion or compliance?

The tendency of highly hypnotizable participants to bias their retrospective perceptual reports in response to instructional demands was reexamined with the addition of low-hypnotizable control participants instructed to simulate hypnosis. Mean scores of high-hypnotizable participants and simulators did not differ, but the responses of simulators to the demand instruction was less variable than those of high-hypnotizable participants, and the shape of the response distribution was different. Unlike simulators, some high-hypnotizable participants who had reported changes in perception that were consistent with a hypnotic suggestion subsequently reported changes opposite to those suggested by a demand instruction. These data were interpreted as suggesting that the responses of high-hypnotizable participants to both the demand instruction and the preceding hypnotic suggestion were not entirely due to compliance.

Adult↗
PubMed1992

Amyloidosis: current approaches for diagnosis and treatment.

Amyloidosis is a complex disease resulting from the extracellular deposition of fibrous degradation proteins in body tissues. The type of protein deposited determines the class of amyloidosis present. Morphological, cytochemical, and immunohistochemical studies allow one to precisely identify the class of amyloid present and, therefore, more appropriately select a therapy. Rectal biopsy is the preferred initial procedure for obtaining tissue for study; however, subcutaneous fat pad, gingival, skin, and bone marrow biopsies are alternative noninvasive biopsy sites. We have presented two patients with multiple myeloma and amyloidosis of the AL type. One patient presented with multi-organ failure of undetermined etiology and the other with asymptomatic hepatosplenomegaly. The diagnosis of amyloidosis was made by bone marrow biopsy in the first patient and rectal biopsy in the second patient using morphological studies and confirmed cytochemically with the congo red stain. The type of amyloidosis was determined with immunohistochemical studies. Their clinical course and response to therapy will be assessed by repeated immunoglobulin studies, liver and spleen size, and studies for organ dysfunction such as 2-D echo and level of proteinuria.

Amyloidosis↗
PubMed1990

Potential for alterations in family process: when a family has a child with cystic fibrosis.

Cystic Fibrosis (CF) is a disease that has profound consequences on a family unit. As a genetically transmitted, chronic illness, it influences every aspect of family life, including time constraints, finances, and relationships among family members. The purpose of this article is to demonstrate the cumulative effect of the nursing diagnoses related to the pathophysiology of CF on the nursing diagnosis, Potential for Alterations in Family Process, when a family has a child with cystic fibrosis. This nursing diagnosis was first recognized by the North American Nursing Diagnosis Association (NANDA) in 1982. It is vital that nurses be able to differentiate between a family having difficulty coping with a child with CF in the home, and one that is coping well on its own. The children of families that are not coping well tend to be hospitalized more than those of families that are coping well. The families use the hospitalizations as rest periods for themselves. By recognizing the cues identified by NANDA of a family having problems, early intervention can be initiated before the family is in a crisis. The family must live with CF on a daily basis for the remainder of the child's life; therefore it must be able to function at its optimum level. It is nursing's responsibility to be able to recognize and assist the family in need. A knowledge of the nursing diagnosis, Alterations in Family Process, will assist nurses in fulfilling this obligation to the family.

Adaptation, Psychological↗
PubMed1979

Comprehensive evaluation model for nursing education.

The proposed model provides structure for determining decisions to be made, questions to be answered, and sources of information about evaluation. A sample of the kinds of decisions that can be made for the various matrix elements of the evaluation model is reflected. Examples of questions that could be asked (why) in order to evaluate various aspects of a program (what) and of whom these questions could be asked (who) are provided. The model as described is useful in comprehensively delineating all aspects of the system. The flexibility of the system allows for the unique needs and aspects of the individual school to emerge. The model also provides a framework for organizing and implementing a plan for total program evaluation.

Data Collection↗
PubMed1978

An in-vitro model of malignant hyperthermia: differential effects of inhalation anesthetics on caffeine-induced muscle contractures.

Clinical concentrations of anesthetics augment caffeine-induced contracture of frog sartorius muscle; however, anesthetics differ in this characteristic. The potentiation was quantitated using six paired sartorius muscles for each specified concentration of anesthetic and controls. At a concentration of 1 MAC, the greatest potentiation occurred with 2 mM caffeine for all anesthetics studied. Under these conditions the order of magnitude of augmentations was: chloroform (15 times); halothane (11 times); methoxyflurane (10 times); cyclopropane (5 times); enflurane (4 times); isoflurane (3 times); diethyl ether (2 times); Baxter 3224 (2 times); fluroxene (1.4 times); nitrous oxide (1.3 times). Halothane at .5 MAC augments the 2 mM caffeine-induced contracture almost seven times, and at 2 MAC almost 13 times, whereas 2 MAC isoflurane potentiates the caffeine-induced contracture only four times and 4 MAC diethyl ether only two and a half times. It is postulated that those anesthetics that most potentiate caffeine-induced contracture may be the most potent triggering agents of malignant hyperthermia.

Anesthetics↗