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S Baba

Publications and source records attributed to S Baba.

At least 1,135 records · Page 63Linked to original sources
PubMed1978

Pancreatitis-like isoamylase pattern in normal persons.

On the assumption that a rise in the pancreatic type isoamylases may not necessarily indicate underlying pancreatitis, genetic studies of human serum and urinary amylase isoenzymes have been performed with the use of electrophoresis. Although the preponderant increase in the two principal pancreatic isoamylases Amylase-1 and 2 has been accepted to be a specific index of pancreatic involvement, 1.68% of normal persons had Amylase-2 with an elevated amylase activity (named "Dominant Amylase-2") up to the same levels as the major isoenzymes. Results of pancreozymin-secretin test and other laboratory findings of these persons with Dominant Amylase-2 were all within normal ranges. Pedigree studies confirmed an autosomal dominant mode of inheritance for this variant. The important of serial determination and pedigree investigations has been shown to distinguish normal persons having Dominant Amylase-2 from patients with pancreatitis without elevated amylase activity. The existence of an inherited trait of pancreatitis-like isoamylase pattern in healthy individuals must be born in mind before coming to a conclusion when amylase isoenzymes are used for clinical medicine, though preponderance of the pancreatic type isoenzymes in serum and urine has been revealed to be a characteristic finding in pancreatitis. Knowledge of amylase genetic polymorphism provides a scientific basis for amylase isoenzyme interpretation.

Acute Disease↗
PubMed1978

[Effect of caerulein on pancreatic endocrine and exocrine secretion from the perfused rat pancreas (author's transl)].

Several investigations in vivo and in vitro have shown that gastro-intestinal hormones stimulate insulin secretion. However, the reports on the insulinotropic activity of pancreozymin are contradictory. The conflicting results are probably due to the fact that pure native preparation of this hormone has not been obtained in "physiologic" doses. In the present study this problem has been investigated by exposing rat pancreas to caerulein in vitro. Caerulein, an active decapeptide isolated from the skin of the Australian amphibia Hyla caerulea, resembles pancreozymin in chemical structure, including C-terminus. This active polypeptide of nonmammalian origin has been shown to possess all the biological activities of pancreozymin. The present investigation was undertaken to evaluate the significance of the interactions of exocrine and endocrine pancreas using perfused rat pancreas in vitro. Biphasic insulin release was demonstrated with caerulein at concentrations higher than 1 ng/ml. Insulin response of the first phase was proportional to the dose up to 1 microgram/ml. The second phase of insulin release was, however, almost constant, regardless of the concentrations of caerulein. Release of glucagon was stimulated by the same concentrations of caerulein which stimulated insulin release. Maximal response of the pancreatic amylase and pancretic juice output were observed with 1 ng/ml of caerulein. With higher doses, significantly less secretory responses were observed. The dissociation of the response to caerulein between endocrine and exocrine pancreas was found.

Amylases↗
PubMed1978

Studies on drug metabolism by use of isotopes XX: Ion cluster technique for detection of urinary metabolites of 1-butyryl-4-cinnamylpiperazine by mass chromatography.

An ion cluster technique and mass chromatography were used for the structural elucidation of several unidentified urinary metabolites of 1-butyryl-4-cinnamylpiperazine (I) in mice and guinea pigs. The urinary metabolites in guinea pigs and mice receiving a single 100 mg/kg dose of an equimolar mixture of 1-butyryl-4-[aromatic-d(5)]-cinnamylpiperazine (I-d(5)) hydrochloride and I hydrochloride (I:I-d(5)) were purified and subjected to trimethylsilyl derivatization with bis(trimethylsilyl)acetamide, followed by GLC-mass spectrometry-computer analysis. Appropriate fragment and molecular ions of unidentified metabolites were selected based on the major mass fragment ions appearing in the mass spectra of the previously identified metabolites of I to provide the mass chromatograms. The presence of ion clusters as doublet peaks in the mass chromatograms indicated that the corresponding total ion peaks originated from the administered I:I-d(5). The mass spectra of selected scans from the total ion chromatogram were plotted, and the structures of unidentified metabolites were readily determined by the presence of ion clusters separated by 3-5 mass units and by the shift of the fragment ions. By using this technique, six previously unidentified metabolites were identified in the urine of guinea pigs and two were identified in the urine of mice.

Analgesics↗
PubMed1978

Clinical evaluation of the pancreatitis-like isoamylase pattern in normal persons.

Amylase isoenzyme analysis of serum and urine has been performed in 4001 normal persons and 500 patients with various disease using electrophoresis on thin layer polyacrylamide gel. Although elevation of amylase activity in amylase-1 and 2 has been reported to be the specific findings in patients with pancreatitis, 1.69% of normal persons had an elevated Amylase-2(named "Dominant Amylase-2") up to the same levels as major isoenzymes (Amylase-1 and 3), along with Amylase-1. Pedigree study confirmed an autosomal dominant mode of inheritance for Dominant Amylase-2. Knowledge of the genetic polymorphism is of importance in clinical assessment of amylase isoenzymes in patients having an elevated Amylase-2 suggestive of pancreatitis. Predominance of the pancreatic components in serum and urine has been revealed to be a specific index of pancreatic involvement. However, the existecne of an inherited trait of pancreatitis-like isoamylase pattern in healthy individuals must be borne in mind. On the basis of the present study, it may be concluded that a rise in the pancreatic type isoenzymes may not necessarily indicate underlying pancreatitis, especially in the absence of elevated amylase and lipase levels.

Acute Disease↗
PubMed1978

Antiandrogenic effects of spironolactone: hormonal and ultrastructural studies in dogs and men.

A decrease in the level of plasma testosterone and an increase in the level of plasma progesterone were noted after spironolactone had been administered for 20 days in 5 patients with prostatic carcinoma, as well as in 8 male dogs. Electron microscopic observation disclosed myelin-like bodies in the cytoplasm of Leydig and adrenocortical cells in dogs, contributing to a resolution of the mode of antiandrogenic action of spironolactone.

Adrenal Glands↗
PubMed1978

Studies on drug metabolism by use of isotopes. 23. Metabolic study of 1-butyryl-4-cinnamylpiperazine in the rat during development of tolerance by using two kinds of deuterium-labeled forms.

Mass fragmentographic determination of the plasma, brain, and liver levels of 1-butyryl-4-cinnamylpiperazine (I) and its metabolites in tolerant and nontolerant rats was carried out by using two kinds of deuterium-labeled compounds. 1-Butyryl-4-[a-d2]cinnamylpiperazine (I-d2) was used to develop tolerance and 1-butyryl-4-[arom-d5]cinnamylpiperazine (I-d5) to clarify the metabolic fate of I after I-d2 administration. The present method allowed the clarification of the metabolism of I-d2 and I-d5 independently. In tolerant rats, the brain and liver were lower for I-d5 and higher for I-d5 metabolites than in nontolerant rats. There was no significant difference in the plasma levels of I-D5 between tolerant and nontolerant rats. Our data seem to suggest that development of tolerance to I results from a more rapid metabolism of the drug due to hepatic enzyme induction.

Animals↗
PubMed1978

Specific IgE estimations by RAST in Japanese asthmatics compared with skin, passive transfer and bronchial provocation tests.

198 asthmatics and twenty healthy persons were studied by RAST and in vivo tests with four common inhalant allergens. (a) Higher RAST classes were elicited with mite (Dermatophagoides farinae) extract and lower classes with other allergens. The agreement between positive RAST and skin or P-K tests was highest with the mite extract. RAST sensitivity was dependent on the kinds of allergens and was most sensitive to mite extract. Positive RAST was most closely related to the prick test reactions. (b) RASTs to house dust and mite extract were examined in terms of the threshold dosage of house dust and also the types of bronchial response (early, dual and late) induced by a certain amount of house dust; higher RAST classes were found in subjects with bronchial response to the dual or early type, elicited by a threshold dosage of diluted extract, while lower RAST classes were found in cases of the late-type response elicited by the threshold dosage of concentrated extract. (c) Changes in IgE antibodies to house dust and mite extract were estimated in patients with positive house dust provocation. A greater increase occurred in cases of dual or early response, a smaller increase in those with a late response. Despite discordances in skin tests and RAST between house dust and mite extracts, the increases in IgE antibodies to mite extracts as well as house dust were observed in all cases, presumably caused by an allergenic identity between the allergens.

Adolescent↗
PubMed1978

Biochemical studies on rats with insulin-secreting islet cell tumors induced by streptozotocin: with special reference to physiological response to oral glucose load in the course of and after tumor induction.

Pancreatic islet cell tumors were induced in 32 of 49 male Wistar rats (73%) surviving 9 months or longer following treatment with streptozotocin alone, with streptozotocin and nicotinamide, or with streptozotocin and picolinamide. Serial oral glucose tolerance tests in rats treated with streptozotocin and nicotinamide showed that the elevation of blood glucose levels after oral glucose load was depressed significantly 7 months after treatment. Plasma insulin responses were distinctly elevated 9 months after treatment. Blood glucose levels remained lower and plasma insulin levels rose markedly after a glucose load in tumor-bearing rats as compared to the response of tumor-free rats. These findings suggest that pancreatic islet cell tumors induced by streptozotocin with and without combined treatment are insulin-secreting, and that streptozotocin itself has oncogenic effects on the rat pancreas. Mean insulin concentration in islet cell tumors amounted to 401 U/g wet wt, whereas the concentration was 14 U/g wet wt in the pancreatic tissue from tumor-free rats.

Adenoma, Islet Cell↗