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S Baba

Publications and source records attributed to S Baba.

1,377 records · Page 77Linked to original sources
PubMedDate not supplied

Further structural analysis of urinary metabolites of suprofen in the rat.

The urinary metabolites of 2-(4-(2-thienylcarbonyl)phenyl)propionic acid (suprofen, S) in rats were analyzed by radio-GC, GC/MS, or 1H NMR technique. Radio-GC analysis of trimethylsilylated materials after TLC separation of intact urine showed the presence of three radioactive peaks with the retention times corresponding to the authentic S, 2-(4-(2-thienylhydroxymethyl)phenyl)propionic acid, and 2-(4-carboxyphenyl)propionic acid. About 40% of the total radioactivity appearing in the 0-24-hr urine was accounted for by the three metabolites and their conjugates. The identification of these metabolites was confirmed by comparison of the MS spectra of urine, in which rats were administered an equimolar mixture of S and S[phenyl-d4], with those of synthetic standards. The labile metabolites of S, corresponding to about 32% of the total radioactivity appearing in the 0-24-hr urine, were isolated and purified by ether extraction from the fresh urine and GC/MS or HPLC. GC/MS of the methylated metabolite revealed the consistent presence of the ion peaks at m/z 304, 245, 217, and 141, indicative of a dimethylated product with monohydroxy group on the thiophene ring. Analysis of the 1H NMR spectrum demonstrated the metabolite to be 2-(4-(5-hydroxy-2-thienylcarbonyl)phenyl)propionic acid.

Animals↗
PubMedDate not supplied

Identification of germ line mutation of APC gene in possible carriers of familial adenomatous polyposis (FAP).

In Japan 1052 FAP patients in 688 family trees have been registered at the National Polyposis Center. In Hamamatsu University School of Medicine, we have been treating 50 patients in 28 family trees. Among these families, we analysed the site of mutations of the APC gene, which so far has been clarified in 21 family trees. Presymptomatic diagnosis was possible in 12 individuals among the carriers in 6 FAP families. Most of the mutations were clustered within the 5'half of exon 15. There was no correlation between extra colonic phenotypic expression and site of mutation, however, the desmoid should be studied further, as the number of cases was limited.

Adenomatous Polyposis Coli↗
PubMedDate not supplied

Extended lymphadenectomy and the quality of life in rectal cancer patients.

For a better quality of life in rectal cancer patients, high dose radiotherapy following abdominoperineal resection of the rectum with a pelvic partition is another surgical option replacing extended abdominoperineal resection. In addition to pelvic partition with polyglycolic acid mesh, the tissue expander was inserted into the pelvic cavity to support the intestine upward and the bladder forward. The mean total radiation dosage was 5040 cGy. Between 1989 and 1991, 10 patients were treated according to this method. Out of 10 patients 9 were free of recurrence, and only one had hepatic metastasis. In addition, postoperatively, the average residual urine by this method was 39.1 ml and was statistically different compared to a figure of 200 ml in conventional abdominoperineal resections (p < 0.001, "t"-test). In order to individualize the operative procedures among a variety of surgical options, the molecular biological technique was utilized. In p53 stain analysis of 114 colorectal cancer patients, patients with p53 positive staining reached a higher stage than those with p53 negative staining (p < 0.05, chi 2 analysis). Therefore, we surmised that the positivity of the p53 stain could be one of the factors gauged as an indication of postoperative high-dose radiation. In conclusion, high-dose postoperative radiotherapy was thought to be one of the treatment modalities to improve the survival and quality of life of advanced rectal cancer in selected cases.

Adult↗
PubMedDate not supplied

Radiotherapy under informed consent report of a long-term survivor of advanced esophageal cancer.

This paper reports a patient with advanced esophageal cancer who has survived asymptomatically for five years after radiotherapy given under informed consent. Previously, we had not practiced radiotherapy under informed consent for patients with esophageal cancers. His excellent clinical course encouraged us to promote this form of treatment. This report presents his excellent clinical course in detail and discusses the significance of informed consent in radiotherapy for malignancies.

Aged↗
PubMedDate not supplied

Resection of metastatic liver tumors with special reference to hepatic venous system.

BACKGROUND/AIMS: When the right and middle hepatic veins (RHV and MHV) and all the short hepatic veins are removed during resection of segments (S) 7 + 8 including the caudate lobe, the remainder of (S) 5 + 6 shows congestion. METHODOLOGY: The subjects were 8 patients undergoing hepatectomy (7 with hepatic metastases of colorectal carcinoma and 1 with pancreatic carcinoid) for tumors located in the region circumscribed by the RHV, MHV, and inferior vena cava. Direct hepatic vein anastomosis was done in 5 patients, graft replacement of the MHV was done in 1 patient, and graft replacement of the RHV was done in 2 patients. RESULTS: Hepatic vein reconstruction took approximately 20 min to complete. All 8 patients had an uneventful postoperative course and the anastomosis was patent at one month after operation. Three patients died of recurrent carcinoma at 6, 30, and 48 months after operation, respectively. Five have remained alive and disease-free for 18, 30, 46, 67, and 79 months, respectively. CONCLUSION: Hepatic vein reconstruction is a beneficial option in hepatectomy for patients with carcinoma invading the major hepatic veins and short hepatic veins, since it can allow radical tumor resection and preserves the remnant liver function. In particular, direct hepatic vein anastomosis is recommended because it is a simple technique with good results.

Aged↗
PubMedDate not supplied

Anxiety-like behavior in transgenic mice with brain expression of neuropeptide Y.

Neuropeptide Y (NPY), one of the most abundant peptide transmitters in the mammalian brain, is assumed to play an important role in behavior and its disorders. To understand the long-term modulation of neuronal functions by NPY, we raised transgenic mice created with a novel central nervous system (CNS) neuron-specific expression vector of human Thy- gene fragment linked to mouse NPY cDNA. In situ hybridization analysis demonstrated transgene-derived NPY expression in neurons (e.g., in the hippocampus, cerebral cortex, and the arcuate nucleus of the hypothalamus) in the transgenic mice. The modest increase of NPY protein in the brain was demonstrated by semiquantitative immunohistochemical analysis and by radioreceptor assay (115% in transgenic mice compared to control littermates). Double-staining experiments indicated colocalization of the transgene-derived NPY message and NPY protein in the same neurons, such as in the arcuate nucleus. The transgenic mice displayed behavioral signs of anxiety and hypertrophy of adrenal zona fasciculata cells, but no change in food intake was observed. The anxiety-like behavior of transgenic mice was reversed, at least in part, by administration of corticotropin-releasing factor (CRF) antagonists, alpha-helical CRF9-41, into the third cerebral ventricle. These results suggest that NPY has a role in anxiety and behavioral responses to stress partly via the CRF neuronal system. This genetic model may provide a unique opportunity to study human anxiety and emotional disorders.

Animals↗
PubMedDate not supplied

Purpuric lichen nitidus.

We report the case of a patient in whom lichen nitidus with hemorrhage was found on the palm and the thigh. Histopathologically, the small vessels in the lesions showed extensive changes and were increased in number. The capillary walls revealed degenerative changes with extravasation of erythrocytes. On electron microscopy, amorphous, electron-dense deposits were observed along the outside of the endothelial cells. These findings can explain the presence of the hemorrhagic changes. We describe this as a case of purpuric lichen nitidus.

Hand Dermatoses↗