PubMed Health⌕ Search

Biomedical subjects

S Bachman

Publications and source records attributed to S Bachman.

10 recordsLinked to original sources
PubMed1997

Degenerate and specific PCR assays for the detection of bovine leukaemia virus and primate T cell leukaemia/lymphoma virus pol DNA and RNA: phylogenetic comparisons of amplified sequences from cattle and primates from around the world.

Degenerate and specific PCR assays were developed for bovine leukaemia virus (BLV) and/or primate T cell leukaemia/lymphoma viruses (PTLV). The degenerate assays detected all major variants of the BLV/PTLV genus at a sensitivity of 10-100 copies of input DNA; the specific systems detected 1-10 copies of input target. Sensitivity was 100% in specific DNA-PCR assays done on peripheral blood from seropositive BLV-infected cattle and HTLV-I- or HTLV-II-infected humans, and 62% in RNA/DNA-PCR assays on sera from BLV seropositive cattle. The pol fragments from 21 different BLV strains, isolated from cattle in North and Central America, were cloned and sequenced, and compared to other published BLV and PTLV pol sequences. BLV and PTLV sequences differed by 42%. Sequence divergence was up to 6% among the BLV strains, and up to 36% among the PTLV strains (with PTLV-I and PTLV-II differing among themselves by 15% and 8%, respectively). Some cows were infected with several BLV strains. Among retroviruses, BLV and PTLV sequences formed a distinct clade. The data support the interpretation that BLV and PTLV evolved from a common ancestor many millennia ago, and some considerable time before the PTLV-I and PTLV-II strains diverged from each other. The dissemination of the BLV strains studied probably resulted from the export of European cattle throughout the world over the last 500 years. The relatively similar mutation rates of BLV and PTLV, after their various points of divergence, suggest that there could be a much wider genetic range of BLV than has currently been defined.

Amino Acid Sequence↗
PubMed1989

Implementation issues in addictions day treatment.

Innovative treatment programs can be implemented successfully in hospitals only if key actors take on leadership responsibility for the new programs. Implementation of an innovative Addictions Day Treatment Program (ADT) for alcohol and substance abusers in a 156-bed community hospital is reported. Implementation issues for staff psychiatrists, psychologists, psychiatric nurses, and hospital administrators are the primary subject of this article. Participation of these professional groups depended on their treatment ideology, the threat they perceived the program to be to patients, the time demanded of them, whether they would be reimbursed for providing care by the program, and the changes the program introduced in provider-patient interaction. The way shared responsibility among key hospital and unit leaders made it possible to overcome resistance to the program and to develop a positive consensus about day treatment is shown.

Alcoholism↗
PubMed1988

Effect of irradiation on the patulin content and chemical composition of apple juice concentrate.

The influence of ionizing radiation on the patulin content of apple juice concentrate was investigated. The results indicated that patulin, at an initial concentration of about 2 mg/kg, disappeared after irradiation of the concentrate with doses as low as 2.5 kGy. For lower doses, the extent of patulin degradation was proportional to the absorbed dose. Irradiation of the concentrate with doses sufficient for patulin disappearance did not change the titratable acidity, the content of reducing sugars and carbonyl compounds or the amino acid composition. The content of ascorbic acid slightly decreased and the colour of the concentrate brightened. The intensity of the patulin absorption spectra after irradiation of mycotoxin in aqueous solutions decreased.

Amino Acids↗
PubMed1983

PHC in Bangladesh--too much to ask?

In Bangladesh where the number of those without land is increasing; 30% of the budget (national) goes for "maintaining law and order'; there is massive illiteracy; the common people and more specifically, women, are not involved in decision-making. It is unrealistic to think that PHC has a chance to survive or succeed. Should Government (with all good intentions) try to run a PHC while socio-economic and political factors remain unchanged, it (PHC) will not succeed. Its fate is either a collision, in which PHC will be the victim, or a compromise of some type. In most Third World countries, it is a compromised PHC that one sees. In spite of this, we still believe that community-oriented health care can help to bring about social, economic and political changes, provided such changes are accompanied by structural reform in the political economy of the country.

Bangladesh↗
PubMed1983

Dietary fiber decreases cholesterol and phospholipid synthesis in rat intestine.

The effects of fiber ingestion on the incorporation of oleic acid into triglyceride and lecithin, acetate incorporation into cholesterol, and monosaccharide and amino acid transport were determined in rat intestine. Prolonged pectin (10% by weight) ingestion caused a decrease in jejunal and ileal cholesterol synthesis (33% and 52%, respectively). Pectin ingestion reduced cholesterol synthesis by 60% in ileal crypt cells, but did not affect cholesterol synthesis in the jejunal or ileal villus cells or in jejunal crypt cells. Cholesterol synthesis in isolated crypt cells was markedly less than in isolated villus cells. Prolonged ingestion of a fiber-free diet supplemented with either cellulose or pectin (10% and 5% by weight, respectively) decreased jejunal lecithin glucose and leucine absorption but did not affect jejunal triglyceride synthesis.

Acetates↗
PubMed1981

Effect of aging on the electrocardiogram.

Previous cross-sectional population studies have shown age differences in electrocardiographic wave patterns, including lower wave amplitudes and a leftward shift of the frontal plane axis in older people. However, cross-sectional results may be due to cohort differences and the data imply only that these changes actually occur in persons as they age. In order to examine electrocardiographic changes with aging in the same persons, serial recordings, obtained 10 years apart, were taken in 440 healthy male participants of the Normative Aging Study, who were 23 to 66 years old on their first examination. At examination 1, R and S wave amplitudes were smaller and frontal plane axis measurements were shifted to the left in older men. Longitudinal changes in these same variables were consistent with the cross-sectional results. In addition, the P-R and Q-T interval durations were longer, the QRS duration was shorter and the T wave amplitude was smaller at the second examination. The longitudinal rate of change of S wave amplitude varied among age groups, decreasing more in younger men. Thus, some previously described cross-sectional age differences truly represent longitudinal age trends in electrocardiographic patterns.

Adult↗
PubMed1979

Failure of prostaglandins E1 and E2 to alter canine gastric mucosal cyclic nucleotides.

The action of prostaglandins and indomethacin on gastric mucosal cyclic nucleotide concentrations was evaluated in 18 anesthetized mongrel dogs. Prostaglandins E1 (PGE1) and E2 (PGE2) (25 microgram/kg bolus, then 2 micrograms/kg/min) were administered both intravenously (4 experiments; femoral vein) and directly into the gastric mucosal circulation (10 experiments; superior mesenteric artery). The possible synergistic effect of pre-treatment and continuous arterial infusion of indomethacin (5 mg/kg bolus for 5 min, then 5 mg/min), a prostaglandin synthetase inhibitor, with PGE2 was studied in 4 experiments. Antral and fundic mucosa were biopsied and measured by radioimmunoassay for cyclic nucleotides. Doses of PGE1 and PGE2 which inhibited histamine-stimulated canine gastric acid secretion did not significantly alter antral or fundic mucosal cyclic nucleotide concentrations. Concomitant infusion of PGE2 with indomethacin did not potentiate the mucosal nucleotide response compared to PGE2 alone. These studies fail to implicate cyclic nucleotides as mediators of the inhibitory acid response response induced by PGE1 or PGE2 in intact dog stomach.

Animals↗
PubMed1978

Effects of secretagogues and theophylline on canine gastric mucosal cyclic nucleotides.

cAMP and cGMP concentrations were measured in gastric mucosa in 18 anesthetized mongrel dogs receiving infusions of histamine (16 microgram/kg/hr), pentagastrin (6 microgram/kg/hr), bethanechol (10 microgram/kg/min), and theophylline (150 mg bolus, then 10 mg/min). The possible synergistic effect of continuous theophylline infusion with the secretagogues was studied in 15 other acute experiments. Antral and fundic mucosa were biopsied and measured by radioimmunoassay for cyclic nucleotides. All the secretagogues did not significantly change antral or fundic mucosal cyclic nucleotide concentrations compared to basal levels. In contrast, phosphodiesterase inhibition by theophylline significantly increased cAMP 11-fold and cGMP threefold in both antrum and fundus. Concomitant infusion of each secretagogue with theophylline did not potentiate the mucosal nucleotide response compared to theophylline alone. In a separate series of 19 experiments in three anesthetized dogs with vagally denervated fundic (Heidenhain) pouches, all the secretagogues which were infused in the same doses as in the acute biopsy studies produced maximal acid secretory responses. Theophylline, on the other hand, failed to stimulate acid secretion in five Heidenhain pouch experiments. These studies fail to implicate cyclic nucleotides as mediators of stimulated canine gastric acid secretion.

Animals↗