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Biomedical subjects

S Benassi

Publications and source records attributed to S Benassi.

9 recordsLinked to original sources
PubMed1999

Angiogenesis in lipoma: An experimental study in the chick embryo chorioallantoic membrane.

Lipoma is one of the most common benign mesenchymal tumors. Its ability to trigger an angiogenic response is a critical step for its growth. Because adipose tissue serves as an important conduit for the vasculature, it is conceivable that the angiogenic properties of this tissue may modulate the growth of the vasculature in a paracrine manner. We investigated in vivo the angiogenic potential of bioptic fragments of human lipoma by using the chick embryo chorioallantoic membrane (CAM), a useful model for such an investigation. The angiogenic response in pathological and control implants was assessed on histologic sections by a morphometric method, 96 h after grafting. Results showed that pathological samples were surrounded by numerous allantoic vessels with a radially arranged pattern around the implant. The vascular counts in the CAMs treated with lipoma implants were comparable to that of FGF-2. The role played in vasoproliferative response by angiogenic cytokines (FGF-2, VEGF) released by adipocytes, by endogenous cytokines, such as FGF-2, stored in the CAM extracellular matrix and by angiogenic growth factors released by perivascular mononuclear cells around the newly-formed blood vessels, were supported by this study.

Adult↗
PubMed1996

Synthesis and biological activity of a series of isoindolone derivatives related to cytochalasins.

A series of hydrogenated isoindolone derivatives structurally related to cytochalasin B has been synthesized and their ability to induce binucleation in the human lymphocytes were tested. All compounds were found able to inhibit cell cytokinesis at different extent in the range (3.12-25 mumol/l) respect to the negative control; however the highest percentage of binucleated cells is induced by Cyt B.

Cell Nucleus↗
PubMed1994

Induction of binucleation in human lymphocytes by 14 synthetic isoindolone derivatives related to cytochalasins.

Fourteen hydrogenated isoindolone derivatives with affinity to cytochalasin B were synthesized and tested for the induction of binucleation in the human lymphocyte micronucleus (MN) assay. The experimental procedure was the one commonly used for the human lymphocyte MN assay. Compounds like cyt-B were added to cultures at 44 h and tested at increasing concentrations, up to 200 mumol/l, in the range commonly used for cyt-B in the MN assay (3 micrograms/ml = 6.25 mumol/l). Induction of cytokinesis-blocked binucleated cells was found for all compounds but, at the same molarity, cyt-B induced a higher percentage of binucleation. Only one of the compounds tested was found to induce micronuclei significantly: MN were induced across the dose range of 25-100 mumol/l.

Cytochalasin B↗
PubMed1992

High-performance liquid chromatographic determination of N-methylformamide and N-methyl-N-(hydroxymethyl)-formamide in human urine.

A reliable high-performance liquid chromatographic (HPLC) method which allows the determination in human urine of two important metabolites of N,N-dimethylformamide (DMF), namely N-methylformamide (MMF) and N-methyl-N-(hydroxymethyl)formamide (DMFOH), is reported. A single-step rapid purification of urine was performed on a C18 solid-phase extraction column and the eluate was injected directly on to the HPLC column. HPLC was carried out isocratically on Aminex Ion Exclusion HPX-87H column using 7.5.10(-4) M sulphuric acid as the mobile phase with ultraviolet detection at 196 nm. The method is specific, accurate, precise and sufficiently sensitive to be applied to the biological monitoring of MMF and DMFOH in workers exposed to DMF.

Chromatography, High Pressure Liquid↗
PubMed1991

Chondrosarcoma of the soft tissues. Two different sub-groups.

Chondrosarcomas arising from soft tissues are rare. Two different varieties are described, myxoid and mesenchymal. We have collected nine cases of the tumour, five myxoid and four mesenchymal, from a review of 513 cases of chondrosarcoma seen between 1904 and 1988. We report the principal clinical, radiographical and histological differences between the two varieties and discuss their surgical treatment and prognosis.

Adult↗
PubMed1981

[Levels of aspartate aminotransferase and alanine aminotransferase in two factories with various hepato-toxic risks].

The Authors have studied AST and ALT enzymatic activities in the workers of two firms, the former of which (tannery) with a high and the latter (boot and shoe factory) with a low level of hepatic-toxic risk. The influence of various trouble factors such as age, sex and seniority was eliminated through appropriate statistical techniques. A significant difference was evidenced between AST and ALT levels in two firms, chiefly attributable to the quantity and quality of the substances utilized in the two technological cycles: trichloroethylene, chromium, sulphuric acid, mineral oils, ammonia, N-hexane, pentanes acetone, ciclo hexane, methanol, ethyl acetate, isopropyl acetate, toluene, methylene chloride.

Adolescent↗