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Biomedical subjects

S Bernáth

Publications and source records attributed to S Bernáth.

18 recordsLinked to original sources
PubMed2005

Haemorrhagic nephritis and enteritis of geese: pathomorphological investigations and proposed pathogenesis.

Haemorrhagic nephritis and enteritis of geese as a new disease was first described in Hungary in 1969. The authors identified the causative agent of the outbreaks occurring in 1969 as a polyomavirus by PCR in 2001. In order to study the pathogenesis of the virus, one-day-old goslings were infected with tissue homogenate that tested positive for polyomavirus by PCR. Morphological, light and transmission electron microscopic (TEM) examinations have revealed that goose haemorrhagic polyomavirus replicates in the endothelial cells of the blood vessels and capillaries of diseased birds. Infection causes damage and necrosis of the endothelial cells. The virus was not observed in the parenchymal cells. Oedema and haemorrhages found throughout the body may be due to the dysfunction or functional deficiency of endothelial cells damaged by the virus.

Animals↗
PubMed2003

BGP-15, a hydroximic acid derivative, protects against cisplatin- or taxol-induced peripheral neuropathy in rats.

The neuroprotective effect of BGP-15 against peripheral sensory neuropathy was studied in rats that were exposed to short-term cisplatin or taxol administration. The changes of nerve conduction velocity were determined in situ after treating the Wistar rats with BGP-15 (50, 100, and 200 mg/kg po daily doses throughout the experiment), cisplatin (1.5 mg/kg ip daily dose for 5 days), or taxol (5.0 mg/kg ip daily dose every other day in a 10-day interval) alone or giving the test compound in combination with cisplatin or taxol. Electrophysiological recordings were carried out in vivo by stimulating the sciatic nerve at both sciatic notch and ankle site. Neither motor nor sensory nerve conduction velocity was altered by any dose level of BGP-15 tested. Both anticancer drugs decreased the sensory nerve conduction velocity (SNCV). BGP-15 treatment prevented the impairment of SNCV either in part or totally in the cisplatin- or taxol-treated groups. This neuroprotective potential of BGP-15 could be well correlated with its recently described poly(ADP-ribose) polymerase- inhibitory effect and its ability to protect against the damages induced by the increased level of reactive oxygen species in response to anticancer treatment.

Animals↗
PubMed2002

Intestinal absorption of colostral lymphoid cells in newborn animals.

Intestinal absorption of colostral lymphoid cells was studied in 23 piglets of four sows and 17 lambs of ewes. From the colostrum and blood of the dams the lymphoid cells were isolated with Ficoll-Paque and labelled with technetium (Na99mTcO4). In the 7th hour after birth, 10 ml volume of the cell suspensions (piglets: 10(7) cells, lambs: 5 x 10(7) cells) were injected, following laparotomy, directly into the stomach or into the jejunum, or through a nasooesophageal tube. Cryostat sections of duodenum, jejunum and lymph node samples of animals were examined by autoradiography. It was found that lymphoid cells present in the colostrum of a piglet and lamb of their own mother were absorbed from the digestive tract and, via the lymphatic vessel, were transported to the mesenteric lypmph nodes. Electron microscopy revealed that absorption takes place intercellularly. Colostral cells of sows other than a piglet's own mother (allogeneic cells), the lymphoid cells isolated from the blood and heat-treated colostral lymphoid cells were not absorbed. The immunization of ewes and their lambs by tetanus anatoxin demonstrate, that the absorbed lymphoid cells remain immunologically active, and may transfer immune information to the lambs.

Animals↗
PubMed2001

Computerized comparison of the protein compositions of Erysipelothrix rhusiopathiae and Erysipelothrix tonsillarum strains.

Protein profiles of six Erysipelothrix rhusiopathiae strains, five Erysipelothrix tonsillarum strains and three Erysipelothrix strains of uncertain taxonomic position were studied by sodium dodecyl sulphate-polyactylamide gel electrophoresis (SDS-PAGE). In a computerized comparison of the protein patterns of the strains, the level of similarity between the strains was determined. The SDS-PAGE protein bands were divided into 14 groups based on molecular weight. The relative distribution of proteins within these groups was used to characterize the strains. These distribution patterns were analysed by computing Pearson's correlation coefficient between strains, and by cluster analysis based on Euclidean distances and the unweighted pair-group method of arithmetic averages (UPGMA). The geometric mean of the similarities calculated by Pearson's correlation coefficient was 0.980 +/- 0.018 between the E. rhusiopathiae strains and 0.979 +/- 0.013 for E. tonsillarum strains. The value was 0.932 +/- 0.036 between the strains belonging to different species. However, a threshold value applicable for identification of a given strain to a species could not be established. Of the three strains of uncertain taxonomic position, the strains designated Rotzunge and Iszap 4 had a protein composition more similar to that of E. tonsillarum than to that of the E. rhusiopathiae type strain. The strain designated Pécs 56, which may be a member of a new species according to literature data, gave inconsistent results by the two methods used. The computerized evaluation method developed here is suitable for the comparison of the protein composition of the strains and for the construction of the protein similarity tree by cluster analysis.

Animals↗
PubMed1998

Computerized evaluation procedure for comparing the electrophoretic protein patterns of bacterial strains.

The advantages and drawbacks of different methods of evaluating electrophoretic (SDS-PAGE) protein patterns were studied by comparing Erysipelothrix rhusiopathiae strains. The computerized evaluation procedure recommended by the authors renders it possible to determine objectively the degree of similarity of the protein composition of the different strains according to molecular mass. The degree of similarity obtained in four parallel analyses of a given strain was 988 +/- 0.55 on a scale on which 1000 equals complete identity. the method can be used without adjustment even in those cases where the two strains to be compared contain unequal amounts of electrophoresed proteins.

Bacterial Proteins↗
PubMed1998

Comparison of the protein composition of Erysipelothrix rhusiopathiae strains of subtype 1A isolated from ducks and pigs.

Erysipelothrix rhusiopathiae strains isolated from duck carcasses and pigs were examined by sodium dodecyl sulphate--polyacrylamide gel electrophoresis (SDS-PAGE) and the results were evaluated by a computerised method in order to compare the strains for protein composition according to molecular mass. To characterise the similarity of the strains, Pearson's correlation coefficient was calculated. The degree of similarity between duck strains originating from the same flock was 980-991, while that of strains isolated from ducks and pigs varied between 836 and 991 on a scale of 0 to 1000. The method gives highly reproducible results and can be utilised in epidemiological investigations. Its use may provide new data on E. rhusiopathiae strains, complementing the results of methods recommended for this purpose earlier and increasing the efficiency of epidemiological investigations.

Animals↗
PubMed1997

Comparison of the protein patterns of Erysipelothrix rhusiopathiae strains by SDS-PAGE and autoradiography.

The proteins of 12 Erysipelothrix rhusiopathiae strains were radiolabelled with L-[35S] methionine, and the protein fractions were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and autoradiography. The strains were found to differ from each other in their protein patterns; thus, it was concluded that the method applied could provide useful data for the identification of strains. The described procedure was used for determining the percentage contents of protein fractions of identical molecular mass within the strains compared. The results show that there is no significant correlation between the type of the strains and the percentage of identical molecular weight fractions.

Animals↗
PubMed1996

Suspected adverse drug reactions in the Hungarian veterinary practice between 1982 and 1992.

The most characteristic adverse drug reactions observed after the use of home-manufactured or imported veterinary medicinal products in Hungary between 1982 and 1992 included toxicosis, severe local reaction, lack of efficacy, and presence of residues in the edible tissues of food animals. The causes of adverse drug reactions comprised manufacturing defects, lack of chemical or microbiological stability, misuse or extra label use, and neglecting the warnings in the directions for use. Collection and analysis of data relating to adverse drug reactions are indispensable for the prevention of similar cases. The authorities can facilitate data collection by supplying veterinary practitioners with the necessary report forms.

Adverse Drug Reaction Reporting Systems↗
PubMed1995

Intestinal absorption of colostral lymphocytes in newborn lambs and their role in the development of immune status.

Two model experiments were conducted to study the intestinal absorption of colostral lymphoid cells and the role of these cells in the development of immune status in newborn lambs. In experiment I, 17 lambs of 14 Merino ewes were used. Suspensions of lymphoid cells separated from the colostrum (cell density: 5 x 10(6)/ml) and blood (3 x 10(6)/ml) were labelled with technetium (Na99mTcO4) of 37 MBq/ml radioactive concentration. In three groups of lambs, 10-ml volumes of the cell suspensions were injected directly into the duodenum after laparotomy, while in a fourth group (group Ia) the same volume was administered to the animals through an oesophageal tube. The labelled cells revealed that colostral cells of the lamb's own dam are absorbed from the gastrointestinal tract and get into the newborn lamb's lymph circulation irrespective of the route of application. In experiment II, involving 40 lambs of 40 ewes, we studied the effect of absorbed colostral lymphocytes on the development of the newborn lamb's immune status. Twenty ewes (group A) each were treated with 3 ml tetanus anatoxin twice, while the remaining animals (group B) were left uninoculated. Lambs of group A (designated A2) were separated from their dams immediately after birth, then were administered, through an oesophageal tube, 10 ml of a suspension of lymphoid cells (cell density: 5 x 10(6)/ml) separated from the maternal colostrum. Subsequently, the lambs were interchanged with lambs of nonimmunized ewes of group B (designated lambs B1), i.e. were mutually put out to nursing. At three days of age, lambs of groups A1, A2, B1 and B2 were inoculated with 3 ml tetanus anatoxin, then blood samples were taken from them 5 times in a period of 27 days for comparative examination of the humoral and cellular immune reactions. The results demonstrate that lymphoid cells from the colostrum of the lambs' own dam become absorbed into the newborn lambs' lymph circulation, remain immunologically active and may transfer, besides immunological memory, also cellular activity.

Animals↗
PubMed1989

Haemolysin producing capacity and mouse-pathogenicity of Listeria monocytogenes.

For studying the relationship between their haemolysin producing capacity and pathogenicity on mice, 12 Listeria strains were investigated, and 9 of them were reinvestigated one year later. Haemolysin production was measured on 51Cr-labelled sheep erythrocytes and the 50% haemolysis-inducing dilution of the bacterial suspensions was calculated. Pathogenicity of the strains was estimated by intraperitoneal injection of mice. In both experimental series the correlation coefficients were calculated (r = 0.408, t = 1.41 and r = 0.163, t = 0.43). Using this experimental system, no correlation existed between the haemoysin producing capacity and mouse-pathogenicity of these strains.

Animals↗
PubMed1988

Intestinal absorption of colostral lymphoid cells in newborn piglets.

Intestinal absorption of colostral lymphoid cells was studied in 23 piglets of four sows (sows A, B, C and D). From the colostrum and blood of the sows the lymphoid cells were isolated with Ficoll-Paque and labelled with technetium (Na99mTcO4). In the 7th hour after birth, 5-ml volumes of the cell suspensions were injected, following laparotomy, directly into the stomach (piglets of sow A) or into the jejunum (piglets of sow B), whereas piglets of sows C and D received the suspensions through a naso-oesophageal tube. Cryostat sections of duodenum, jejunum and lymph node samples of piglets killed by bleeding 8 h after the treatment were examined by autoradiography. It was found that lymphoid cells present in the colostrum of a piglet's own mother were absorbed from the digestive tract and, via the lymphatic vessels, were transported to the mesenteric lymph nodes. Electron microscopy revealed that absorption took place intercellularly. Colostral cells of sows other than a piglet's own mother were observed only in the epithelial layer of the mucous membrane. The lymphoid cells isolated from the sows' blood and heat-treated colostral lymphoid cells were not absorbed. The results indicate that in the pig, an animal having an epitheliochorial placenta, the colostral lymphoid cells are absorbed from the digestive tract and, hence, they can confer an active cellular immunity on the newborn piglets.

Animals↗
PubMed1978

Mathematics of the inverse solid-phase radioimmunoassay.

Use of a simplified, idealized model allows a mathematical treatment of the inverse solid phase radioimmunoassay. Equations are presented for calculation of the following values: bound/free antibody ratio (R) in the state of equilibrium; the equilibrium constant (K), the concentrations of the directly non-measurable values (Abf; AbfAg: Abs and Ag) as well as the concentration of the antibody tested [Abf]0. The model has the same parameters as the test system, thus the equations may be used in planning of the test in the laboratory.

Antitoxins↗
PubMed1976

Solid-phase radioimmunoassays for quantitative antibody determination of bacterial exotoxins. Measurement of Clostridium perfringens type D epsilon antitoxin.

Two reversed solid-phase radioimmunoassays have been developed for quantitative determination of antibodies against Clostridium perfringens type D epsilon toxin. 125I labelled prototoxin was used in the bromoacetyl cellulose-bound antibody method and in the antibody coated tube method. The procedures are based on the competition for 125I labelled prototoxin between the insoluble antibodies and the antibodies present in the unknown sample. The radioactivity bound to solid-phase is in inverse ratio to quantity of measured antibodies. The antibody values which can be detected are in the range of 0.004 IU/ml of investigated serum. The methods allow the rapid and inexpensive screening of large groups of vaccinated sheep, and are very suitable for measuring small amounts of Cl. perfringens D epsilon antibodies with a small experimental error.

Animals↗
PubMed1975

Preparation, measurement and possible use of human antitoxin against Cl. botulinum A, B, and E toxins.

Human antibodies against botulinum toxins A, B, and E have been raised by repeated injections of pentavalent toxoid in a healthy volunteer. The final titer was 3.2 U anti-A, 0.4 U anti-B, and 2.5 U anti-E/ml. In mice, the efficacy of the antitoxin decreases with the time between poisoning and application of the antiserum. The dose recommended for prophylactic purposes in man is 1 ml/kg. In overt poisoning, therapy should be started with higher titer animal serum since in animal experiments high titer sera can stop (although not reverse) the symptoms of overt botulism within an-admittedly not too broad-rnage of time and dosage. Later on, therapy can be continued with human antiserum. An inverse radioimmunassay for botulinum A antitoxin using labeled botulinum toxin and antibody-coated tubes is described. The serum is available upon request from the author.

Animals↗
PubMed1975

Solid-phase radioimmunoassays for quantitative antibody determination of Clostridium perfringens type D epsilon toxin.

Two reversed solid-phase radioimmunoassays were developed for quantitative determination of antibodies against Clostridium perfringens type D epsilon toxin. 125I-labeled prototoxin was used in the bromoacetylcellulose-bound antibody method and in the antibody-coated tube method. The antibody values which can be detected by the assays are in the range of 0.004 IU/ml of investigated serum. The methods allow the screening investigation of large groups of vaccinated sheep in a rapid and inexpensive way, and are very suitable for measuring small amounts of C. perfringens D epsilon antibodies with a small experimental error.

Animals↗