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Biomedical subjects

S Bernander

Publications and source records attributed to S Bernander.

25 records · Page 2Linked to original sources
PubMed1982

Antigenic similarities and differences in genus Pityrosporum.

Using the indirect immunofluorescence technique no differences were observed in intensity of staining when antisera against Pityrosporum orbiculare and P. ovale or IgG fractions of these were incubated with P. orbiculare and P. ovale cells. However, a weaker reaction was observed with these antisera and P. pachydermatis cells. With IgG fractions the nonspecific fluorescence was minimized. Fungal cells in biopsies from tinea versicolor lesions showed a brilliant staining with the IgG fractions of antisera against P. orbiculare. This investigation indicates a close antigenic relationship between all of the 3 Pityrosporum species. A common antigenicity was found between P. orbiculare and P. ovale cells as well as between P. orbiculare cells from culture and the fungal cells in biopsies from tinea versicolor lesions. This strongly suggests that these 2 species are identical with each other and with the organism found in tissue.

Animals↗
PubMed1981

Solubility of antimycotics: a problem in in vitro experiments.

An investigation of how miconazole nitrate dissolved in dimethylformamide in initial concentrations of 1,000, 500, and 100 microgram/ml may alter its concentration with storage time, is presented. The determination of miconazole nitrate was performed with high pressure liquid chromatography. After 8 hours the 1,000 microgram/ml solution was milky and the concentration of miconazole had fallen significantly. It was concluded that test agar plates or agar tubes must be made with freshly prepared and clear stock solutions, otherwise false MIC's may be obtained.

Dimethylformamide↗
PubMed1981

Micro-aerophilic and anaerobic growth of Pityrosporum species.

Pityrosporum orbiculare, P. ovale, and P. pachydermatis showed the same luxuriant growth when cultured under aerobic conditions, in an aerobic milieu with 7% CO2, and under micro-aerophilic conditions. The species survived and even multiplied under strictly anaerobic conditions. These findings may explain how P. orbiculare and P. ovale can survive in the deeper parts of hair follicles. On a medium that enhanced the production of filaments in Pityrosporum, filaments were more pronounced in the micro-aerophilic environment. Macroscopically, P. ovale showed a more luxuriant growth than P. orbiculare on this medium, but microscopically the same type of filaments were produced.

Aerobiosis↗
PubMed1979

The activity in vitro of five different antimycotics against Pityrosporum orbiculare.

The activity in vitro of miconazole, clotrimazole, econazole, sodium omadine, and sodium thiosulphate against Pityrosporum orbiculare was found to correlate with the good clinical results these drugs produce in tinea versicolor. In addition many substances used as solvents or in vehicles had an inhibitory effect in vitro against P. orbiculare. The influence of the culture medium, especially lipids, on the action of imidazole derivatives is discussed.

Antifungal Agents↗
PubMed1978

Prevalence of hospital-associated infections in five Swedish hospitals in November 1975.

The prevalence rate of hospital-associated infections in 5 Swedish hospitals on November 4, 1975 is reported. In all, 4246 patients were included in the study, 3657 in acute disease clinics and 589 in chronic disease clinics. The overall prevalence rate was 17%, 11% in acute disease clinics and 59% in chronic disease clinics. The highest rate was found in intensive care units (72%), while in ophthalmological units it was 1%. 50% of all hospital-associated infections were urinary tract infections, 68% of which occurred in patients with an indwelling urinary catheter. 25% of all infections in acute disease clinics were postoperative wound infections, and 20% in chronic disease clinics were skin infections, including infections in varicose and decubital ulcers. 58% of the bacterial isolates from hospital-associated infections were gram-negative rods, while 12% were Staphylococcus aureus. Also in postoperative wound infections the gram-negative rods dominated over Staph. aureus, 35 vs. 23%. A prevalence study of this order of size seems adequate to assess the overall rate of hospital-associated infections in Sweden as compared to other countries. However, differences in prevalence rates between hospitals and clinics should be interpreted with great care. The seriousness and effect of reported infections must be evaluated otherwise, as well as the day-to-day infection control and the evaluation of prophylactic measures need other methods.

Acute Disease↗