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S Bozsóky

Publications and source records attributed to S Bozsóky.

32 records · Page 2Linked to original sources
PubMed1979

Antibodies to native DNA in connective tissue disease. A comparison of radioimmunoassay, counterimmunoelectrophoresis and indirect immunofluorescence on Crithidia luciliae substrate.

Sera of patients with symptoms of connective tissue diseases were investigated for the presence of antinuclear antibodies by indirect immunofluorescence on rat liver substrate. In positive cases antibodies to native DNA were determined by radioimmunoassay and by counterimmunoelectrophoresis. Twenty seven selected sera were also tested by indirect immunofluorescence on Crithidia luciliae substrate to test the sensitivity and specificity of this method. Immunofluorescent antinuclear antibodies could be found in all groups of connective tissue diseases while anti-native DNA antibody was demonstrated in higher amount only in the cases of systemic lupus erythematosus and therefore determination of anti-native DNA antibody may be helpful in the diagnosis of oligosymptomatic SLE. Counterimmunoelectrophoresis and immunofluorescence on Crithidia smears may serve as screening methods for anti-native DNA while radioimmunoassay provides a quantitative determination, although there are minor differences in the sensitivity and the specificity of these three methods.

Adolescent↗
PubMed1979

Rheumatoid factors bind beta 2 microglobulin. Detection of beta 2 microglobulin-complexes in rheumatoid arthritis.

beta 2 Microglobulin binding rheumatoid factors and occurrence of complexed form of beta 2 microglobulin were found in rheumatoid arthritis (RA) by radioimmunological methods. 1. As related to the appropriate values of control sera and osteoarthritic synovial fluids, one of the six sera and five of the six synovial fluids of RA patients exhibited increased binding activity for 125I-beta 2 microglobulin, 2. Rheumatoid factors (RF) purified by means of immunoadsorbent bound beta 2 microglobulin; 3. Both 19S and 7S components of sera, synovial fluids and purified RF samples had beta 2 microglobulin binding activity, which was inhibited by an excess of unlabeled beta 2 microglobulin; 4. Complexed (3% PEG 6000 insoluble) beta 2 microglobulin was found in three of fourteen sera and in twelve of fourteen synovial fluids of RA patients. Level of complexed beta 2 microglobulin was independent of its total concentration in the original samples; 5. In the fraction of synovial fluids enriched immune complex the amount of beta 2 microglobulin was in positive correlation with quantity of RF. Presumable immunological and pathological significance of "anti-beta 2 microglobulin"-like cross-reactive RF antibodies is discussed.

Arthritis, Rheumatoid↗
PubMed1978

Clinical immunological study of T lymphocytes and EA rosette forming cells in tonsils.

Tonsillar E and EA rosette forming lymphocyte subpopulations were studied in 120 tonsillectomized patients. T cell ratios was usually lower in the tonsils than in the blood. EA binding cells were studied with indicator systems of human or rabbit antibody sensitized red cells, respectively. Poorly sensitized human RBCs (EArabbit) bind much better to tonsillar cells than to blood lymphocytes and so this system proved to be specially suitable to study tonsillar EA binding cells. Increase in E and EA rabbit rosette forming cell frequencies were found with the age of patients. Decrease in percentage of T cells and EA rabbit rosette binding cells were found with high frequency of acut tonsillitis and with clinical sysmptoms of chronic local inflammation. The variation of these lymphocyte subpopulations with the local tonsillar inflammatory processes suggest a considerable clinical immunological role of local T cells and of this portion of Fc receptor postive lymphocytes.

Adenoids↗
PubMed1978

[Study of C3 and C4 complement components of synovial fluids in connective tissue diseases (author's transl)].

C3 and C4 levels and the relative concentration of C3 degradation products were measured in fifty pairs of synovial fluids and sera of patients who suffered from different connective tissue diseases. Inverse correlation between C4 level and C3 breakdown products were found in synovial fluids of different groups. Compared to the other groups of patients, the highest increase of C3 degradation was found in RA. patients and it occurred in the SF all of these patients. In arthrosis cases we failed to find any degradation of C3. Of the seven investigated ankylosing spondylitis only two showed some degree of activation of C3 in the SF. Our results suggest that the occurrence and degree of degradation of C3 complement component is a valuable laboratory parameter in the study of connective tissue diseases.

Arthritis↗
PubMed1977

Differential binding activity of EA receptor bearing human lymphocyte subpopulations.

Two types of EA rosettes were studied in rheumatoid arthritis patients and control subjects. Heterologous (rabbit) and homologous (anti-D) antibody sensitized human red blood cells (EAr and EAh, respectively) were used for examining the EA binding capacity of human lymphocytes. Both control and rheumatoid arthritis blood samples yielded significantly lower EArRFC levels as compared to the percentage of EArRFCs, the difference being even more expressed with control lymphocytes. No difference, however, was observed in the case of tonsillar lymphocytes, as equal rosette levels were reached in both systems used. Introduction of immunosuppressive therapy resulted in a temporary dissociation of the two rosette values EAr and EAh binding lymphocytes were differently affected by aggregated human IgG, only the latter ones being inhibited, contrasting with the equal inhibitory action of purified Fc fragments. Reducing the density of anti-D antibodies on the surface of erythrocytes, the EAh rosette count dropped to the level of EAr RFCs. The data suggest an activity-depending Fc receptor distribution resulting in different binding capacity for more or less sensitized indicator RBCs.

Animals↗
PubMedDate not supplied

[Functional analysis of immune complex-rich serum fractions in autoimmune diseases by in vitro C3 activation of fresh blood].

A method, measuring the activity of circulating immune complexes, based on C3 conversion of fresh human plasma by means of 3 per cent PEG precipitable immune complex-rich sera fractions is reported. Sera of patients suffering from autoimmune diseases of the connective tissue as well as of healthy controls were investigated. In case of standardised protein concentration of immune complexes, significantly low C3 conversion frequency of the isolated fractions has been observed in the control group as compared to the examined patients (rheumatoid arthritis with and without extraarticular manifestations, or juvenile chronic arthritis, or systemic lupus erythematosus). PEG fractions of RA with extraarticular manifestations induced the significantly highest C3 splitting. It is assumed that immune complexes will show a distinct interaction with the complement system also in vivo, and are therefore directly implicated in the systemic course of the disease process.

Arthritis, Juvenile↗