Biomedical subjects

S Buchner

Publications and source records attributed to S Buchner.

21 records · Page 2Linked to original sources

PubMed1981

The deoxyglucose method for insects: towards electron microscopical resolution.

The problems of extending the deoxyglucose method for insects to electron microscopical resolution are discussed. It is calculated that several curies of 3H-deoxyglucose per kilogram body weight may be needed for identification of activity in ultra-thin sections. Oral application may be superior to injection. Incorporation of experimental and control stimulus in the same animal seems appropriate. Freeze-drying of Drosophila results in structural preservation sufficient for cell identification in the cervical connective. Autoradiography of ultra-thin sections has not yet been carried out.

Animals↗

PubMed1980

Mapping stimulus-induced nervous activity in small brains by [3H]2-deoxy-D-glucose.

Nervous activity may be localized in anatomical sections of brain tissue by the autoradiographic deoxyglucose technique. The method provides sufficient structural preservation and spatial resolution for detailed functional investigation of complex but small-sized nervous systems when the original technique is modified as follows: (i) use of 3H instead of 14C as radioactive label, (ii) application of labeled deoxyglucose in concentrations close to physiological glucose levels rather than in trace amounts, (iii) stimulation for 4--9 h after deoxyglucose application instead of 20--45 min, (iv) subsequent preparation avoiding aqueous phases at all stages from fixation to autoradiography, and (v) plastic embedding of the tissue such that serial semithin sections of good structural preservation may be routinely cut. Brief aqueous fixation and dehydration at room temperature as has been described for vertebrates apparently cannot preserve stimulus-induced distribution of radioactive label in the brain of the fly Drosophila melanogaster. Aspects of the results that illustrate the potential and some limitations of the present technique are discussed.

Animals↗

PubMed1979

2-Deoxy-D-glucose maps movement-specific nervous activity in the second visual ganglion of Drosophila.

Adult Drosophila were fed with tritium labeled deoxyglucose prior to a 5-hour period of visual stimulation. A flickering disk of light and a moving grating were presented to the left and right eyes, respectively. Autoradiography revealed enhanced labeling solely in that part of the second optic ganglion (medulla) whose visual field was stimulated by movement.

Animals↗