Biomedical subjects

S C Marselos

Publications and source records attributed to S C Marselos.

2 recordsLinked to original sources

PubMed2002

Development and optimization of a reversed-phase high-performance liquid chromatographic method for the determination of piperacillin and tazobactam in tazocin injectable powder.

A reversed phase high-performance liquid chromatographic method with detection at 220 nm was developed and validated for the determination of piperacillin, I, and tazobactam, II, in Tazocin injectable powder. Acetaminophen was used as internal standard. A Hypersil BDS RP-C(18) column (250 x 4.6 mm), 5 microm particle size, was equilibrated with a mobile phase composed of aqueous solution of sodium dihydrogenphosphate-dihydrate (20 mM)-acetonitrile-methanol (70:15:15, v/v/v) and pH 5.0. Its flow rate was 1.0 ml/min. Calibration curves were linear for I and II in the concentration ranges of 3.0 x 10(-7)-2.0 x 10(-4) M and 7.0 x 10(-7)-2.0 x 10(-4) M, respectively. Limits of detection and quantitation were 1 x 10(-7), 3 x 10(-7) M for I and 2 x 10(-7), 7 x 10(-7) M for II, respectively. Relative standard deviation, for I and II was less than 0.40 and 0.75%, respectively. Extensive recovery studies were also performed.

Chromatography, High Pressure Liquid↗

PubMed2002

Development and validation of a reversed-phase high-performance liquid chromatographic method for the determination of ethyl-3-(N-n-butyl-N-acetyl)aminopropionate in an insect repellent semi-solid formulation.

A reversed-phase high-performance liquid chromatographic method with detection at 220 nm was developed and validated for the determination of ethyl-3-(N-n-butyl-N-acetyl)aminopropionate, IR 3535, in an insect repellent semi-solid product. A Hypersil ODS RP-C18 column (250 x 4.6 mm), 5 microm particle size, was equilibrated with a mobile phase consisted of water-acetonitrile (60:40, v/v). Its flow-rate was 1.0 ml/min. Excipients did not interfere with the determination of IR 3535 (Rs = 8.663). Intra- and inter-day relative standard deviations for samples were not higher than 0.61 and 1.2%, respectively. Mean recovery was found not lower than 98.5% and not higher than 100.3%. The method of external standard was adopted. Calibration curves were linear in the concentration range between 1.0 x 10(-6) and 5.0 x 10(-4) M. Limits of detection and quantitation were 65 and 196 ng/ml, respectively.

Calibration↗