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Biomedical subjects

S C Wang

Publications and source records attributed to S C Wang.

At least 19 recordsLinked to original sources
PubMed1992

FK-506 inhibits proliferation and IL-4 messenger RNA production by a T-helper 2 cell line.

FK-506 is a potent immunosuppressant which is known to decrease the generation of allospecific cytotoxic T cells, possibly through its effect on cytokine production. FK-506 has been reported to have a suppressive effect on cytokine synthesis and secretion by pooled lymphocytes; its action on more selective subsets of lymphocytes is unknown. We currently report the effect of FK-506 on proliferation and IL-4 messenger RNA production by the differentiated mouse T-helper 2 cell line Ly1+2-/9. Ly1+2-/9 cells were grown in culture and treated with (1) media alone, (2) Con A, (3) FK-506, and (4) Con A + FK-506. Proliferation to Con A was determined by treating cells with 10 micrograms/ml Con A +/- FK-506 and measuring [3H]TdR uptake. Reverse transcriptase-polymerase chain reaction amplification was used to quantitate the level of IL-4 mRNA expression. FK-506 markedly inhibits Con A-induced proliferation and IL-4 mRNA production by the T-helper 2 cell line Ly1+2-/9. The ability of FK-506 to block the proliferation and IL-4 production by this helper cell subset suggests that this effect may contribute to its observed marked immunosuppressive properties in vitro and in vivo.

Cell Division↗
PubMed1992

[Lung hamartoma: a report of 30 cases and review of 477 cases].

507 cases of lung hamartoma (30 cases reported and 477 reviewed) were analysed. 467 cases of them were of intrapulmonary type, 30 were endobronchial, 8 were multiple and 2 were diffuse. 505 were benign and 2 malignant. The male to female ratio was 1.74:1. The age span was from infant to 67 years with a mean of 41.4 years. 44.3% of the lesions showed no symptoms. The frequently seen symptoms were cough and chest pain. X-ray findings of the intrapulmonary type were characterized by sharply outlined round or oval mass (87.9%), with diameters 3.0 cm (62.1%), lobulation (33.5%), calcification (23.6%). These tumors grew slowly. The average doubling time for 16 cases was 4.2 years. The diagnosis was confirmed in only 18% cases preoperatively. The other cases were misdiagnosed as lung cancer, tuberculoma, metastatic tumors of the lung, etc. The etiological causes, classification, diagnosis, differential diagnosis and treatment of the tumors were discussed.

Adolescent↗
PubMed1992

Purification of radioiodinated human insulin by high performance liquid chromatography for a sensitive radioimmunoassay.

The optimal sensitivity of a radioimmunoassay depends on the purity of the radiolabeled antigen. The conventional purification methods are not complete and are time consuming. The combination of a Sep-pak C18 cartridge and high performance liquid chromatography (HPLC) for the purification of 125I-labeled insulin in our study revealed that the Sep-pak cartridge can serve as the preliminary step to remove unreacted radioactive iodide, the reactants, and labeled but presumably damaged materials unadsorbed to the cartridge. The fractions eluted from the Sep-pak containing high radioactivity and high immunoreactivity to the antibody were chosen for further purification by HPLC to eliminate undesirable radiolabeled substances with a lesser immunoreactivity. The purified radiolabeled insulin was used to develop a sensitive radioimmunoassay with detecting limits of 0.03 microU/mL per tube.

Chromatography, High Pressure Liquid↗
PubMed1992

[Studies on the chemical constituents of Annona squamosa].

Twelve compounds were isolated from Annona squamosa. Their structures were identified as liriodenine (AS-1), moupinamide (AS-2), -(-)-kauran-16 alpha-ol-19-oic acid (AS-3), 16 beta, 17-dihydroxy-(-)-kauran-19-oic acid (AS-4), anonaine (AS-5), 16 alpha, 17-dihydroxy-(-)-kauran-19-oic acid (AS-6), (-)-isokaur-15(16)-en-17,19-dioic acid (AS-7), squamosamide (AS-8), 16 alpha-methoxy-(-)-kauran-19-oic acid (AS-9), sachanoic acid (AS-10), (-)-kauran-19-al-17-oic acid (AS-11), daucosterol (AS-12). Among them, AS-8 is a new amide, AS-9 is a new natural product.

Antineoplastic Agents↗
PubMed1992

[Combined use of glucose and rest period between noise exposure to reduce noise--induced hearing loss].

This paper reports the way which protects hearing from harmful effects of noise. 67 guinea pigs were divided into four groups, 8-9 guinea pigs in each group. The total time of noise exposure at 125 dB SPL white noise in each group was one hour. The control was exposed continuously for 1 hour. Group I was exposed to noise for 30 minutes after intraperitoneal injection of glucose at 2 mg/g body wt., then rest for 30 minutes and exposed for 30 minutes again. Group II was exposed continuously to noise for 1 hour after intraperitoneal injection of glucose at 2 mg and 4 mg/g body wt. Group III was exposed to noise for 30 minutes, rested for 30 minutes and exposed for 30 minutes again without glucose injection. The auditory brain stem response (ABR) was measured before, immediately, 3 days, and 7 days after noise exposure. The effect on hearing conservation was evaluated according to ABR threshold. We observed that the auditory threshold in group I was significantly lower than that of the control in the given time after noise exposure (P less than 0.05), but not so in group II and III. Combined use of glucose and rest period between noise exposure was able to decrease the threshold shift by 15 dB and 10 dB immediately and 7 days after noise exposure respectively.

Animals↗
PubMed1991

Ferrioxamine B derivatives as hepatobiliary contrast agents for magnetic resonance imaging.

Succinyl (SDF), phenylsuccinyl (PSDF), glutaryl (GDF), and phenylglutaryl (PGDF) derivatives of desferrioxamine B (DF) have been synthesized. In rats given the 59Fe(III) chelates of each these ligands at tracer levels, 82-94% of the 59Fe was eliminated within 1-2 days. 59Fe given as DF, SDF, and GDF chelates was excreted primarily in the urine, while nearly 50% of that given as PSDF and PGDF was excreted in the feces. Correspondingly, Fe-DF, Fe-SDF, and Fe-GDF (0.2 mmol/kg) produced early, marked renal, but no gastrointestinal magnetic resonance imaging (MRI) enhancement. Fe-PSDF and Fe-PGDF (0.2 mmol/kg) produced marked and rapid MRI enhancement of the upper small intestine. In animals with cannulated bile ducts, 59Fe from 59Fe-PGDF (carrier added, 0.1 mmol/kg) appeared rapidly in the collected bile, but not in the intestinal contents, proving that the contrast agent reaches the bowel via the bile. These changes in the excretion and MRI enhancement patterns brought about by the presence of a phenyl substituent apparently were not related to changes in lipophilicity or protein binding.

Animals↗
PubMed1991

The study of serum apoprotein levels as indicators for the severity of angiographically assessed coronary artery disease.

Serum apoprotein A-I (Apo A-I) and B (Apo-B) concentrations were determined in 40 subjects undergoing coronary angiography for past myocardial infarction and angina pectoris, and the authors studied the relationship between the apoprotein concentrations and the severity of coronary artery disease (CAD). During this study, serum total cholesterol, triglyceride, and high-density lipoprotein, low-density lipoprotein, and very low density lipoprotein cholesterol concentrations were determined to control analysis. The results showed that the decrease in serum Apo A-I levels was the best indicator distinguishing CAD from non-coronary artery disease; the Apo B/Apo A-I ratio had the most consistent association with the severity of CAD as assessed by angiography; Apo B/Apo A-I values ranging from 0.98 to 1.00 might be considered critical values for early CAD.

Adult↗
PubMed1991

[Activation of neutrophils in allergic asthmatics].

Neutrophil chemotactic activity (NCA) and activity of myeloperoxidase (MPO) of the neutrophils in asthmatic patients during attacks and in remission and normal controls were determined. The results showed that NCA and activity of MPO in asthmatic patients during attacks were significantly higher than those in remission and in the controls. There was no difference of either NCA or the activity of MPO between asthmatic patients in remission and the controls. There was definite correlation between NCA and activity of MPO in asthmatic patients during attacks, but no correlation between the asthmatic patients in remission and the controls. The results suggest that chemotaxis and activation of neutrophils by neutrophil chemotactic factor may play an important role in pathophysiology of asthma.

Adolescent↗
PubMed1991

Innervation of the knee joint in the rabbit and the Formosan rock-monkey (Macaca cyclopis): a retrograde HRP study.

The number and segmental distribution of cell bodies of sensory afferents and sympathetic efferent innervating to the knee joint of the rabbit and the Formosan rock-monkey were investigated using retrograde transport with horseradish peroxidase (HRP). After injecting HRP into the articular knee joint capsule of the rabbits, labeled neurons were found in the ipsilateral L4-S2 dorsal root ganglia (DRG). However, following injection of HRP into the articular cavity of the knee joint in the rabbit and the monkey, labeled neurons were found in both the ipsilateral DRG (L5-S2 and L4-S1 of the rabbit and monkey, respectively) and in the ipsilateral sympathetic ganglia (SG) (L4-S3 (rabbit) and L3-S1 (monkey)). The majority of labeled neurons within the DRG and the SG were composed of medium and large neurons in the monkey and the rabbit, respectively. The present findings suggest that the sensory projections from and sympathetic projection to the knee joint in rabbits and monkeys are similar, but that both projections of monkeys were "shifted" one segment cranially compared to the rabbit on both projections.

Animals↗
PubMed1991

Soft tissue expansion in the treatment of scar alopecia in preschool children.

Indigenous rubber silicon gel soft tissue expanders were used to treat 5 preschool children with scar alopecia. The scar resection rate wa 100%; no severe complication happened. The average healing time was 25 days after the improvement of technical method. This method is simple, effective and the expansion duration is short.

Alopecia↗
PubMed1991

[Clinical and experimental study on treatment of anorexy in children with the activating spleen prescription].

The treatment of 488 cases with anorexy in children showed that the curative effect of the group using Chinese medicines based on the differentiation of symptoms and signs by (1) activating the Spleen, (2) invigorating and activating the spleen was significantly higher than the control using concentrated vitamin B complex (P less than 0.001). The results of the experimental study were as follows: Erbao instant granules (the medicine for activating the Spleen) had the effect of raising the D-xylose excretion rate of urine; increasing the ratio of T-lymph cells in blood; raising the quality of 8 mineral elements in hair and the quality of SIgA in saliva; adjusting the abnormal peristalsis of the experimental rabbits and promoting the ability that duodenums which had been separated from rabbits had to absorb different amino acids and glucose. Jian'er syrup (the medicine for invigorating and activating the Spleen) had the effect of raising the quality of 14 mineral elements in hair; increasing the ratio of T-lymph cells in blood; increasing the index of thymus and spleen in the experimental rats and stimulating them to produce hemolysin. The authors tend to think that the therapeutic principle of activating the Spleen can improve appetite, help the body to absorb and utilize various nutrients which contain many kinds of essential trace elements.

Animals↗
PubMed1990

Nifedipine pretreatment for autonomic dysreflexia during electroejaculation.

Ten spinal-cord-injured males who exhibited autonomic dysreflexia during electroejaculation were given sublingual calcium channel blocker nifedipine pretreatment to prevent this complication. Nifedipine was successful in decreasing peak systolic, diastolic, and mean blood pressures during the procedure. The use of nifedipine resulted in fewer aborted trials and allowed higher energy delivery during the procedure.

Administration, Sublingual↗