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Biomedical subjects

S C Wilson

Publications and source records attributed to S C Wilson.

At least 19 recordsLinked to original sources
PubMed1997

A simple method to measure compartment pressures using an intravenous catheter.

A simple method was investigated to measure compartment pressures using 16-ga intravenous catheters with or without side ports attached by arterial line tubing to a pressure transducer. Pressure measurements from the experimental catheters were within 4 mm Hg of the slit catheter for 99% of all readings, and pressure measurements from the Stryker device were within 5 mm Hg of the slit catheter for 95% of all readings. The addition of one or two side ports to the experimental catheters did not alter the pressure readings. This method is comparable in accuracy to the slit catheter and in simplicity to the Stryker device.

Catheterization↗
PubMed1996

Screening the environmental fate of organic contaminants in sewage sludges applied to agricultural soils: 1. The potential for downward movement to groundwaters.

The potential for organic contaminants present in sewage sludge to leach and cause groundwater contamination following sludge application to agricultural land has been assessed. Models used to predict compound mobility in soil on the basis of physico-chemical parameters were applied to a range of contaminants prioritised and/or detected in sludge and a provisional list of potential "leachers' compiled. In addition, theoretical soil water concentrations following sludge application were calculated using mean reported sludge contaminant concentrations and soil/water partition coefficients. These estimated aqueous phase concentrations were compared with Dutch groundwater quality standards in the absence of appropriate UK standards to identify those compounds which could be present in groundwater at levels of concern. The two prioritised lists were used to identify compounds in sludge which could pose a possible threat to groundwater. Appropriate experimental data were not available to qualify model results. However, the screening exercise indicated that under routine operation practice with typical sludge application rates, and the usual range of compound concentrations detected in sludge, groundwater quality standards were unlikely to be exceeded. However, data variability, reliability and scarcity limited the usefulness of this screening approach.

Agriculture↗
PubMed1996

Surgical management of soft tissue lesions associated with pelvic ring injury.

Mortality rates associated with pelvic ring injury combined with open wounds have decreased considerably during the past 2 decades. Consequently, increased survivability has heightened the demand for definitive stabilization techniques to address pelvic ring instability. Control of hemorrhage and avoidance of sepsis remain paramount concerns in the initial and later stages of management, respectively. Exclusion of occult and readily apparent perforations of the genital urinary and gastrointestinal tracts is essential when using a multidisciplinary approach. Recognition of open and closed degloving injury patterns and appropriate adherence to treatment guidelines will optimize outcome and avoid catastrophic complication.

Fractures, Closed↗
PubMed1996

Improved outcomes for very low birth weight infants associated with the use of combined maternal corticosteroids and tocolytics.

OBJECTIVE: To determine the effects of maternal antenatal treatment with tocolytics, corticosteroids and the use of combined tocolytics and corticosteroids on the morbidity and mortality of very low birth weight infants. STUDY DESIGN: This retrospective study was conducted on all infants born in Tennessee in 1989 and 1990 who weighed < 1,500 g at birth and had no serious malformations. Registered nurses traveled to the delivery hospitals of all study subjects and abstracted information using a structured data collection form. Mortality was ascertained through the computerized linkage of birth and death certificates. Multiple logistic regression analysis was used to control for covariates. RESULTS: As compared to infants whose mothers received no treatment, infants whose mothers received both corticosteroids and tocolysis had a reduced risk of infant (odds ratio 0.38, 95% confidence interval 0.25-0.58) and neonatal mortality (OR 0.32, CI 0.19-0.51) as well as a reduced risk of seizures (OR 0.46, CI 0.23-0.93). Restricting the analysis of infants at 24-28 weeks' gestation and < 1,000 g at birth revealed similar findings regarding mortality. CONCLUSION: The use of combined corticosteroids with tocolytics was associated with better neonatal outcomes than the use of corticosteroids alone, tocolytics alone or no treatment.

Adrenal Cortex Hormones↗
PubMed1994

Comparison of primate and canine models for bone ingrowth experimentation, with reference to the effect of ovarian function on bone ingrowth potential.

Bone growth into porous composite (mesh-bead) titanium plugs was compared in elderly (postmenopausal) female monkeys and female dogs as a means of validating the cross-species interpretations so often made between data from research on dogs and human applications. The effect of oophorectomy on bone ingrowth in the canine model was defined by the comparison of data on fractional ingrowth in animals that had had oophorectomy and in control animals that had had a sham operation. No significant difference in bone growth into the experimental plugs was identified between the two animal models, which lends credence to cross-species interpretation of existing data from dogs. The presence or absence of active ovaries did not affect the ingrowth fraction in the canine model; this suggests that existing data are not confounded by the lack of control of ovarian function. Estrogen depletion does not appear to influence bone ingrowth adversely.

Animals↗
PubMed1994

Development of an experimental model of hypothyroidism in cockatiels (Nymphicus hollandicus).

Hypothyroidism is a possible predisposing factor in a number of disorders of companion psittacine birds. We developed and validated a thyroid-stimulating hormone (TSH) response testing protocol for cockatiels (Nymphicus hollandicus), using 0.1 IU of TSH/bird given IM, with blood sample collection at 0 and 6 hours after TSH, and a commercial radioimmunoassay for thyroxine (T4). This protocol was used to document a seasonal sex difference in stimulated T4 values--females responded with higher T4 values than those in males in summer--and a stress-induced depression of baseline T4 values was detected in a group of cockatiels with normal TSH response. An experimental model for mature-onset hypothyroidism in cockatiels was created by radiothyroidectomizing cockatiels with 3.7 MBq (100 microCi) of 131I/bird given IV. Induction of the hypothyroid state was confirmed by baseline T4 concentration, TSH response test results, thyroid pertechnetate scintigraphy, and gross and microscopic examinations. Classical signs of hypothyroidism (eg, hypercholesterolemia, obesity, poor feathering) were lacking or mild at 48 days after thyroid ablation.

Animals↗
PubMed1993

A histologic and biochemical comparison of the interface tissues in cementless and cemented hip prostheses.

Thirty-four membranes from 20 cementless and 14 cemented prostheses from 33 patients excised during revision arthroplasty were analyzed for foreign-body reactive processes. As a control, identical analyses were performed using adjacent pseudocapsular tissue taken from 12 patients during revision arthroplasty. Histologically, cementless membranes tended to contain more metal debris, and cemented membranes contained more foreign-body giant cells. Collagenase, gelatinase, prostaglandin E2, and interleukin-1 were measured in conditioned media taken from organ cultures of each interface membrane. Both cementless and cemented membranes released significant levels of gelatinase and collagenase, prostaglandin E2, and interleukin-1 into the conditioned media when compared with control tissues. However, the tissue concentrations were not significantly different between cementless and cemented membranes. Although histologic differences between cementless and cemented interface membranes exist, both share a similar capacity to release bioactive products that can induce bone resorption and cause aseptic loosening of the hip prostheses.

Adult↗
PubMed1992

Immunodominant antigens of zoospores from ovine isolates of Dermatophilus congolensis.

Zoospores of Dermatophilus congolensis were analysed by SDS-PAGE and western blotting. The electrophoretic profiles of zoospores from 13 isolates of D. congolensis were similar but not identical when stained with Coomassie blue or silver. Immunodominant polypeptides with apparent molecular masses of 76 and 31 kDa were identified in western blots of 13 of 13 and 12 of 13 isolates respectively of D. congolensis reacted with hyperimmune, ovine, antizoospore sera. Identical immunodominant polypeptides were observed in western blots reacted with sera obtained from naturally infected sheep. Initial characterisation of the 76 and 31 kDa polypeptides indicated that they were probably surface exposed because (i) antibodies eluted from the surface of live zoospores after adsorption of hyperimmune antizoospore serum, reacted principally against the 76 and 31 kDa subunit polypeptides in western blots, (ii) adsorption of hyperimmune antizoospore serum with live zoospores resulted in significant diminution of reactivity against both the 76 and 31 kDa polypeptides in western blots, (iii) indirect fluorescent immunostaining of zoospores with antiserum prepared against gel-purified 76 kDa polypeptide, resulted in intense staining of the zoospore outer coat. Immuno-gold electron microscopy of negatively stained zoospores with antiserum prepared against gel-purified 31 kDa polypeptide identified this antigen as a flagella subunit.

Actinomycetales↗
PubMed1992

Irreducible "pulled elbow" in a child. A case report.

"Pulled elbow" is a common pediatric injury that occurs when axial traction is applied to an arm that is extended while the forearm is pronated. These forces can create a transverse tear in the annular ligament, which permits subluxation of the radial head. In most cases, closed reduction can be performed by supinating the forearm and flexing the elbow. In the case of a five-year-old boy, surgical reduction of this injury was necessary to reduce the subluxated radial head.

Child, Preschool↗
PubMed1991

Diurnal changes in the plasma concentrations of LH and hypothalamic contents of LHRH-I and LHRH-II in the domestic hen.

Diurnal changes of LH secretion in sexually immature hens of 9, 11, 13 and 15 weeks of age consisted of 25-40% increases in the mean concentrations of LH in plasma between 15.00 and 18.00 h, i.e. between 2 h before and 1 h after the onset of darkness. During this time there was a tendency for the mean contents of LHRH-I in the anterior hypothalamus and posterior hypothalamus to increase by 21-74% and 20-56% respectively. In hens of 9 and 15 weeks, diurnal changes in the plasma concentration of LH closely paralleled those of LHRH-I content in the posterior hypothalamus. In contrast, the diurnal rhythm of LH secretion in hens of 11 and 13 weeks was more marked and plasma concentrations of LH continued to rise steeply between 18.00 and 21.00 h, i.e. between 1 and 4 h after the onset of darkness. At 11 weeks, this was associated with a reduction (P less than 0.01) in the contents of LHRH-I and LHRH-II, particularly in the anterior hypothalamus. In laying hens, a diurnal decline (P less than 0.01) in the plasma concentration of LH between 1 and 4 h after the onset of darkness was preceded by a fall (P less than 0.05) in the content of LHRH-I in the posterior hypothalamus and in the total hypothalamic content of LHRH-II (P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1990

Differential responses of hypothalamic LHRH-I and -II to castration and gonadal steroid or tamoxifen treatment in cockerels.

Changes in the hypothalamic contents of LHRH-I and LHRH-II were determined in intact and castrated cockerels injected i.m. with gonadal steroids or tamoxifen. An increase in the plasma concentration of LH after castration was accompained by a significant increase in the content of LHRH-I in the posterior hypothalamus (including the mediobasal hypothalamus and median eminence) which was reversed by oestradiol benzoate given on days 14 and 15 after castration. Under similar circumstances, testosterone propionate did not modify the hypothalamic content of LHRH-I, even though both steroids reduced the plasma concentrations of LH to levels below those of intact cockerels. Treatment of intact cockerels with oestradiol benzoate significantly increased the content of LHRH-I in the posterior hypothalamus, whilst testosterone propionate was again without effect. Tamoxifen significantly raised the plasma concentration of LH in intact cockerels and partially antagonized the suppressive effect of oestradiol benzoate and testosterone on LH secretion in castrated cockerels. However, an anti-oestrogenic effect of tamoxifen on the hypothalamic content of LHRH-I was not demonstrated. There was no evidence of any changes in the hypothalamic content of LHRH-II after castration, with or without gonadal steroid replacement. A change in the hypothalamic content of LHRH-I in response to manipulation of the steroid environment would imply an involvement of this peptide in the mechanism by which gonadal steroids regulate the release of LH. The absence of changes in the hypothalamic content of LHRH-II in the same circumstances suggest that it is not directly involved in the control of LH secretion by the gonadal steroid negative feedback loop.

Animals↗