Synovial fluid fibronectin.
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Fibronectins↗
Biomedical subjects
S Carsons
Publications and source records attributed to S Carsons.
Detection and quantitation of fibronectin in synovial fluid from patients with rheumatic disease.
Fibronectin, a high molecular weight glycoprotein that binds to glycosaminoglycans, fibrinogen or fibrin, and collagen, was identified and quantitated in synovial fluid from patients with various rheumatic diseases. Fibronectin concentration was significantly higher in synovial fluids from patients with rheumatoid arthritis (RA) (697 microgram/ml) than in other synovial fluids. In RA, synovial fluid fibronectin concentration exceeded that in plasma and did not directly correlate with synovial fluid concentrations of albumin, total protein, or fibrinogen. Synovial fluid fibronectin concentration correlated directly with synovial fluid white blood cell count (r = 0.50, P less than 0.005). Fibronectin was present in cryoprotein formed from four rheumatoid synovial fluids.
Arthritis, Rheumatoid↗
Synovial extracellular matrix II. Specific incorporation of immunoglobulin into the cell-free matrix of pannus.
To determine whether immune complex-like material is incorporated into the extracellular matrix (ECM) of proliferated RA synovium, cell-free matrices were isolated from pannus removed at joint replacement surgery, and were subjected to differential extraction. When the IgG and albumin concentrations in the ECM extracts were compared to those in simultaneously obtained synovial fluids, the IgG was found to be enriched 8.8-fold. Approximately 95% of the IgG was extractable with 6M Guanidine-HCl and 8 M Urea-B-ME. Further extraction with collagenase and low-pH buffers did not result in any additional recovery of IgG. Matrix-associated IgG demonstrated a restricted mobility on IEF with a pI of 4.8. The extracellular matrix of RA pannus is enriched in an acidic IgG species. Incorporation of IgG appears to be secondary to non-covalent interactions and may represent an additional reservoir of immune complex material in the rheumatoid joint.
Albumins↗
Synovial extracellular matrix: partial characterization of matrix components and identification of type VI collagen molecular forms.
Alterations in the synovial extracellular matrix (ECM) in chronic inflammatory arthritis may affect synoviocyte adhesion and may be important in the regulation of pannus growth and invasion. Immunohistochemical examination of synovium has revealed enhanced deposition of fibronectin and collagen in interstitial areas consistent with expansion of the ECM in inflammatory arthritis. In order to functionally characterize the ECM of synovium, synovial extracellular matrix was purified from pannus removed at joint replacement surgery. A prominent 140 Kd 6M guanidine extractable component was found to mediate synoviocyte adhesion in a cell-blotting assay. This 140 Kd component was identified as a form of type VI collagen. Type VI collagen appears to be a significant component of synovial ECM.
Cell Adhesion Molecules↗
Quantitation and distribution of vitronectin in synovial fluid and tissue of patients with rheumatic disease.
OBJECTIVE: Our study was undertaken to determine the quantity and pattern of distribution of vitronectin (Vn) in the synovial fluid and tissue of patients with rheumatic disease. METHODS: We quantitated synovial fluid Vn levels in 37 patients (17 with rheumatoid arthritis (RA), 12 with crystal induced arthritis (CIA), and 8 with osteoarthritis (OA)) using a competitive-binding ELISA: Immunofluorescence studies were performed on synovial pannus tissue specimens from 3 RA patients. SDS-PAGE analysis of the synovial fluids was performed to demonstrate the molecular forms of Vn present in inflammatory and noninflammatory synovial fluids. Albumin levels of synovial fluids were determined using an automated chemistry analyzer. RESULTS: Immunoreactive Vn was detected in 36 of 37 synovial fluid specimens examined, over a wide range of dilutions. Concentrations of Vn in inflammatory synovial fluid were significantly elevated compared to non-inflammatory synovial fluid and normal human plasma (NHP). Immunofluorescence studies revealed immunoreactive Vn most heavily concentrated in the lining layers of RA pannus tissue. Similar to NHP, molecular forms of Vn were heterogeneous in the synovial fluid; however, the inflammatory milieu appears to predispose Vn to cleavage at its protease-sensitive site. Overall there was a positive correlation of synovial fluid Vn to albumin. CIA and OA revealed a very strong relationship, whereas RA synovial fluid Vn levels correlated poorly to the levels of albumin. CONCLUSION: Vitronectin is present in synovial fluid. Levels are significantly enhanced in the inflamed joint and in the RA synovial lining, where it may influence cell adhesion and modulate tissue repair.
Arthritis, Rheumatoid↗