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S Chakurov

Publications and source records attributed to S Chakurov.

13 recordsLinked to original sources
PubMed1993

Complete template-directed enzymatic synthesis of a potential antisense DNA containing 42 methylphosphonodiester bonds.

P alpha-Methyl thymidine triphosphate was prepared through the pyrophosphorolysis of P alpha-methyl thymidine diphosphate P beta-diphenyl ester and tested as an alternative substrate for E. coli DNA polymerase 1 (Klenow fragment) using several template-primer systems requiring the formation of 1 to 42 methylphosphono diester bonds. The enzyme catalyzes the incorporation of a P-methyl thymidylic residue with (Sp)-configuration at a single site in a recessive 3'-end as well as at multiple sites along a growing 167 nucleotide long chain. The synthesis of a full length product, containing 42 sites of methylphosphonate incorporation was observed.

Base Sequence↗
PubMed1979

[Clinical chemical parameter changes in patients with liver metastases].

Certain biochemical serum parameters: GOT, GPT, LAP, HK, AP and Regan isoenzyme, GGTP, CE, ESR, Weltman test, thymol test, serum bilirubin and urine urobilogen were determined in 39 patients with different localization of malignant processes in the abdominal cavity (stomach, large intestines, pancreas, ovaries). The patients were subdivided into two groups, depending on the presence or absence of liver metastases, confirmed at laparatomy, laparascopy or necropsy material examination. The results revealed that in patients with liver metastases AP, CE, GOT, GGTP, ESR and Weltman test are most commonly and simultaneously abnormal. In patients without liver metastases, those indices are also changed but to a lesser degree, whereas LAP and Regan isoenyzme are with elevated activity in a higher per cent of these cases, than in the patients with liver metastases, being in unison with literature data. The determination of the above biochemical parameters could direct the clinicist to the presence of liver metastases but the more reliable diagnostic methods as laparascopy and laparatomy cannot be substituted for.

Alanine Transaminase↗
PubMed1975

[Changes in the physiol working capacity of rats following splenectomy and autologous transplantation of part of the spleen].

The author made splenectomy on 15 rats, but on other 15 rats after splenectomy he performed autotransplantation of 1/3 of the spleen in omentum majus. All rats induced the duration of swimming for one month after the operation. Two months later the rats with autotransplantation received the duration of swimming, but the splenectomized rats were under the initial values with 27%. These results showed that auto-transplantation of a portion of the spleen assured the working capacity of the examined animals.

Animals↗