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Biomedical subjects

S Csata

Publications and source records attributed to S Csata.

At least 19 recordsLinked to original sources
PubMed1994

Transfected lymphocyte extracts of patients with urological tumours: complement temperature-sensitive adenovirus mutants in vitro.

Patients with renal or bladder cancers exhibit a unique association with adenovirus (Ad) infections. About 60% of them contain antibodies to Ad early antigens. Both in their tumour cells and peripheral blood lymphocytes (PBL) they have detectable early Ad antigens known to be involved in malignant cell transformation. Transfection of tumour cell extracts resulted in complementing temperature-sensitive (ts) Ad mutants at nonpermissive temperatures (39 degrees C) indicating that some cells of the tumour mass possess active functions for Ad. Only 4 to 18% of control subjects were positive in these tests. Here we studied whether lymphocytes might be involved in tumourigenesis by Ad. PBL extracts of patients were transfected into HEp-2 culture cells, which were subsequently superinfected with Ad-5 ts18 and ts19 mutants at 39 degrees C. Titration of virus yields indicated complementation in 76% of patients with renal and bladder cancers in contrast to 20% of control individuals. Complementing ability of lymphocytes which had been prestimulated with phytohaemagglutinin (PHA) approached that of tumour extracts. It means that both specimens contain advanced functions in contrast to resting lymphocytes. Lymphocytes are nonpermissive for latently carried Ad infections. Expression, possible transfer of early Ad gene products via frequent contacts with tissue cells can result in removal of tumour suppressor gene products from complexes regulating cell cycle negatively. Further interaction with hormone-sensitive protooncogenes explains tissue, age and gender specificity of urological malignancies. These phenomena suggest an important cofactorial role for Ad in kidney and bladder tumours.

Adenoviruses, Human↗
PubMed1993

Follow-up of the effect of BCG vaccine treatment in bladder tumour patients.

The effect of intravesical BCG vaccine treatment in 38 patients with superficial bladder tumour after TUR (Transurethral Resection) was followed by the lymphocyte transformation test (LTT) and the staphylococcus phagocytosis test of in vitro washed leukocytes. The results have confirmed the immunostimulating and hence anti-tumour effect of intravesical BCG vaccine. Monitoring of the cellular immune response is suitable for the continuous follow-up of the BCG effect. Comparing the tolerable side-effects with their favourable therapeutic results, BCG vaccine is considered to be effective for the prevention of recurrences in treating superficial bladder tumours.

BCG Vaccine↗
PubMed1992

Use of pefloxacin in urogenital infections.

Forty-three patients suffering from acute or chronic urinary tract infections have been treated with pefloxacin at the Department of Urology of St. Istán Hospital. Twenty-three patients received oral doses (2 x 400 mg), 1 tabl. in the mornings and 1 in the evenings. Eighteen patients were given 2 x 1 intravenous ampoule (2 x 400 mg) dissolved in 250 ml 5% glucose in slow drip infusion. Both the oral and intravenous doses were given in the patients for 10 days. In two patients complications was observed--in one case rhagades were formed on the lip and face due to photo-sensibilization, in the other case the drug administration had to be discontinued because of nausea and vomiting. These symptoms ceased after the discontinuance of the drug. The clinical conditions, the routes of administration, clinical and bacteriological results are summarized in tables. On the basis of a low number of cases it has been concluded that pefloxacin is a new valuable antibacterial drug the use of which resulted in recovery in 15 out of 18 patients' acute and chronic infections of the urogenital system.

Administration, Oral↗
PubMed1991

Virus-host studies in human seminal and mouse testicular cells.

Virological studies were performed for male infertility and repeated abortions. It was found that in about 40% of infertile males infectious adeno- or herpes simplex virus is present in the semen, while the same viruses are present in 60% of the cells in a latent form. In the aborted fetuses the same viruses could be observed as in the father's cells. Due to semen added to tissue cultures the latent presence also of other viruses could be supposed. The affinity of adeno- and herpes viruses to urogenital organs was confirmed by animal experiments, where a new method was applied for in vivo infection. The close correlation between viruses and urogenital cells was similarly confirmed by infecting in vitro human seminal and testicular cells with adeno- and herpes simplex viruses absorbed by the cells, entering them, moreover replicating in the form of their components. Based on the results, it was assumed that chronic local urogenital infections, due to various viruses, may play a role in male infertility and in a part of repeated abortions by damaging their cells. Applying a Zovirax therapy, the seminal cells were stopped to be viral carriers and normal function was restored.

Abortion, Habitual↗
PubMed1991

Investigations into virus carriership in human semen and mouse testicular cells.

Virological investigations were performed to approach the mechanism of male infertility and recurrent abortions. Infectious adenovirus or herpes simplex virus was found in nearly 40% of semen samples obtained from sterile men. The viruses were detected in latent form in 60% of the cells. The same viruses as in the father's cells were found in the aborted material. In vitro inoculation of cell cultures with material of semen origin made the presence of other latent viruses probable. The affinity of adeno- and herpesviruses to urogenital organs was supported by animal experiments in which a new method of in vivo intratesticular inoculation was applied. The close interaction between urogenital cells and viruses was also supported by in vitro infection of human semen with adeno- or herpesviruses. The viruses absorbed to cells, penetrated the latter and their components replicated inside. It is suggested that local chronic viral infection may play part in male infertility and some cases of the recurrent abortions through damaging of cells. In patients treated with Zovirax, virus carriership ceased and the normal function of sperms started again.

Adenoviruses, Human↗
PubMed1991

Isolation of an infectious oncogenic adenovirus strain from the stimulated lymphocytes of a patient with bladder tumour.

In attempts to grow viruses from tumour cells and circulating lymphocytes, we isolated an oncogenic infectious adenovirus type 18 strain from the phytohaemagglutinin-stimulated peripheral T-lymphocytes of a patient with bladder tumour. The virus genome is assumed to play certain role in the development of the tumour in interaction with other DNA viruses and the damaged immune system.

Adenoviruses, Human↗
PubMed1991

Follow-up of the effect of BCG in bladder tumour patients.

The intravesical BCG effect in 38 patients with superficial bladder tumour after TUR was followed by the lymphocyte transformation test (LTT) and the staphylococcus phagocytosis test of in vitro washed leukocytes. The results have confirmed the immunostimulating and hence anti-tumour effect of intravesical BCG. The beginning and the duration of the stimulation effect were defined and the necessity of maintenance treatment was verified. Authors consider monitoring of the cellular immune response suitable for the continuous follow-up of the BCG effect. Comparing the tolerable side-effects with their favourable therapeutic results, BCG is considered to be suitable for the prevention of recurrences in treating superficial bladder tumours.

Administration, Intravesical↗
PubMed1990

Successful culturing of infectious, oncogenic adenovirus from the stimulated lymphocytes of a patient with bladder tumour.

During their attempts at culturing viruses from tumour cells and circulating lymphocytes, authors obtained oncogenic type 18 infectious adenovirus from the phytohaemagglutinin-stimulated, peripheral T lymphocytes of a patient with bladder tumour. They found that the lymphocytes of other patients with urogenital tumour often show sensibility to the viral antigens. The successful culturing of the virus proves that patients with urogenital tumour carry the functioning genomes of the ontogenic virus, not only in their tumour cells but also in their circulating lymphocytes. It is assumed that these genomes jointly with the immune system impaired by other DNS viruses might have a role in tumour development. It is known that the tumour cells and circulating lymphocytes of patients with malignant urogenital tumour may carry viral components, latent viruses. However, infectious virus--in the mentioned cases--could not be isolated. In the present study attempt was made to culture infectious virus from the tumour cells and the in vitro stimulated circulating T lymphocytes of patients with urogenital tumour.

Adenoviruses, Human↗
PubMed1987

The effect of cell extracts of urological tumours on adenovirus mutants.

Various tumours of the urogenital system were studied whether their cells contain gene products characteristic of functioning adenovirus genes. Complementation studies were made by using tumour cell extracts and two temperature-sensitive adenovirus mutants at restrictive and permissive temperatures. The procedure was applied in in vitro HEp-2 cell culture. It was found that cell extracts of bladder tumour, renal tumour, malignant bladder papilloma and of penis cancer are capable of complementing adenovirus mutants at restrictive temperature, i.e. they induce the reproduction of virus mutants being otherwise incapable of replication. Thus, functioning adenovirus genes are present in the tumour cells. It seems that virus genes together with hormonal changes activate the cell protooncogenes giving rise to and maintaining tumours.

Adenocarcinoma↗
PubMed1987

Latent virus carrying lymphocytes of patients with urological tumours.

A considerable wealth of data suggest that a virus with oncogen character can play a part in the development or survival of tumours, by disturbing immune reaction. We studied this situation in urogenital tumours. Examining the latent virus carrier with an immunofluorescent method in 96 cases with malignant tumours and 70 control cases, it was found that more than 50% of the patients had latent adeno- or herpes simplex virus antigens in 1-3% of the circulating lymphocytes, whereas virus carriers occurred in hardly a few percent in the control group. Using a lymphocyte transformation test, the non-specific mitogen phytohaemagglutinin was able to transform only 10-50% of the lymphocytes of patients with malignant tumours into lymphoblast cells (the percentage also depending on the stage of the tumour). On the other hand, under the influence of phytohaemagglutinin 55-85% of the lymphocytes of the control group turned into lymphoblasts. The lymphocytes of the majority of patients with tumours became sensitive for specific adeno- and herpes simplex virus antigens, mainly the lymphocytes of those whose cells were virus carriers.

Adenoviruses, Human↗
PubMed1987

Complementability of temperature-sensitive adenovirus mutants with extracts of urogenital tumour cells.

Complementation of two temperature-sensitive (ts) mutants of adenovirus type 5 was attempted with tumour extract in HEp-2 cell cultures at permissive and restrictive temperature. Both ts mutants were successfully complemented with adenovirus gene products extracted from bladder and kidney carcinoma cells. The percentage incidence of complementation appeared to be higher with extracts obtained from female patients than with those obtained from males. The same gene sequences may occur in penile carcinoma cells and in bladder carcinoma cells showing the early signs of malignization. Extracts of seminoma cells and of cells from prostatic hypertrophies contained adenovirus genes less frequently; infectious virus was never obtained from them. The same applies to cells from the non-tumourous ("control") patients tested. The old age of the patients and some properties of the tumours examined suggest that hormonal changes may contribute to adenovirus gene expression and, indirectly, to the malignant process and its continuity.

Adenoviruses, Human↗
PubMed1987

Experiences on the surgical management of testicular torsion.

The authors performed operations in 9 patients at the Department of Urology of the István Hospital during the past 5 years. In 7 cases, detorsion and saving of the testicle was successful in possession of an early diagnosis. Two patients had to be castrated. In view of their results, authors draw attention to the necessity of establishing a rapid diagnosis and to the importance of an early surgical intervention.

Adolescent↗
PubMed1985

Immunological and virological studies of patients with tumours of the urogenital system.

The possible connections between the oncogenic viruses and the various tumours of the urogenital system were investigated. Examining the patients' cellular immune response by the lymphocyte transformation test, it was found that the non-specific immune response was considerably diminished in almost all cases, while response to adeno- and herpesvirus antigens was enhanced. Structural and functional impairment of T-lymphocytes frequently occurred. Antiviral humoral antibodies were present more rarely in cancer patients than in the controls. However, type 12 markedly oncogenic anti-adenovirus antibody was frequently found. Using the immunofluorescent method, adeno- and herpesviruses were found in more than 50% of the patients' malignant tumour cells. Electron microscopically, in some cases adeno-, herpes- and type C virus particles, too, were isolated in the tumour cells. According to these results besides other factors also adenoviruses may play a role in urogenital tumours.

Adenoviruses, Human↗
PubMed1984

Renal cell carcinoma--xenotransplantation into immuno-suppressed mice.

21 human renal cell carcinomas (RCC) were xenotransplanted into artificially immunosuppressed mice. 4 tumors grew successfully retaining some characteristics of the primary tumors (according to morphology and karyotype analysis), but losing metastatic capacity. One of the serially transplantable tumors (HT 40) with hyperdiploid cellular DNA content and estrogen receptor positivity failed to respond to the single maximally tolerated dose of several cytotoxic agents.

Adenocarcinoma↗
PubMed1984

Viral examinations in malignant tumours of the urogenital system.

Various tumours of the urogenital system were investigated to elucidate the virus-tumour link. Out of 105 tumours, virus antigens were found in 57 cases by the immunofluorescent method--mainly with serum raised against oncogenic adenovirus type 12. Virus antigens were also present in peripheral lymphocytes of several patients. Occasionally, adeno-, herpes- and C-type virus particles were revealed electron microscopically. Antiviral antibodies were often shown in the patient' sera and in higher titres especially against adenovirus type 12 early non-virion antigens. Herpesvirus antigens were found in 6 cases of 40 controls with hypertrophy of the prostate, and in 2 cases of 15 non-tumorous specimens.

Adenoviridae↗
PubMed1983

Virological studies of malignant tumours of the urogenital system.

Tumours of the urogenital system were investigated to clarify an eventual virus-tumour connection. From 105 tumours virus antigens were found in 57 cases by immunofluorescent method, mainly with serum raised against the oncogenic type 12 adenovirus. Virus antigens were also present in peripheral lymphocytes of some patients. Occasionally electron microscopy revealed adeno-, herpes and C-type virus particles. In patient sera, antiviral antibodies were shown often and in high titre especially against adenovirus type 12 early non-virion antigens. Herpesvirus antigens were found in 6 cases among 40 prostate hypertrophy control patients, and in 2 among 15 non-tumourous specimens.

Adenoviruses, Human↗
PubMed1982

Study of antibodies to adenoviruses in patients with tumors of the urogenital system.

The presumable relationship between viruses and malignant disease has been studied. Approximately 4000 assays for antibodies to adenovirus were carried out with sera of patients with malignant and nonmalignant diseases of the urogenital organs and other organic diseases. The search was directed in the first place at antibodies to the early non-virion antigens of adenovirus-12 of oncogenic properties. On the evidence of the complement fixation reaction antibodies to adenoviruses were found to be less frequent in malignant tumors and in hypertrophy of the prostate than in the control group. Antibody to the non-virion antigen of adenoviruses was found in 53 per cent of patients with neoplastic diseases and prostatic hypertrophy, in 18 per cent of patients with urogenital diseases other than tumors and in 4 per cent of those with other organic diseases. The results point to a possible role of adenoviruses in tumors of the urogenital organs.

Adenoviruses, Human↗