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Biomedical subjects

S Cullheim

Publications and source records attributed to S Cullheim.

At least 73 records · Page 4Linked to original sources
PubMed1991

Calcitonin gene-related peptide in monkey spinal cord and medulla oblongata.

The distribution of calcitonin gene-related peptide (CGRP)-immunoreactive (IR) fibers and cell bodies was studied in the spinal cord and the medulla oblongata of the grey monkey (Macaca fascicularis) using peroxidase-antiperoxidase (PAP) immunohistochemistry. At all levels of the spinal cord many CGRP-IR motoneurons and fibers were seen in the motor nuclei. In the medulla, CGRP-IR cell bodies were encountered in nucleus raphe obscurus, nucleus raphe pallidus and nucleus raphe magnus, nucleus reticularis lateralis as well as in the area dorsal to the inferior olive. Bulbar motoneurons were much more intensely stained than spinal cord motoneurons, indicating higher levels of CGRP-like immunoreactivity (LI) at the medullary level. The concentration of CGRP-LI measured by radioimmunoassay showed higher levels in the dorsal quadrants as compared to the ventral quadrants, but the dorsal/ventral ratio was lower than has previously been reported from the rat. The present results demonstrate that using the PAP technique CGRP-LI can be visualized in a larger number of spinal cord motoneurons of the monkey than earlier revealed by immunofluorescence. Moreover, the finding supports the view that the CGRP-IR nerve endings in the spinal motor nuclei originate from cell bodies in the medullary raphe nuclei.

Animals↗
PubMed1991

Distribution of 125I-galanin binding sites, immunoreactive galanin, and its coexistence with 5-hydroxytryptamine in the cat spinal cord: biochemical, histochemical, and experimental studies at the light and electron microscopic level.

The distribution of galanin-like immunoreactivity (GAL-LI) in the spinal cord of the cat was studied by use of indirect histochemistry and the peroxidase-antiperoxidase (PAP) technique. In the ventral horn GAL-immunoreactive (IR) axonal fibers and terminals were most frequent in the ventral part of the motor nucleus. The GAL-IR axons also contained 5-hydroxytryptamine (5-HT)-LI, and they disappeared after spinal cord transection. It was concluded that these GAL-IR fibers belong to the serotoninergic bublospinal pathway. In the medulla oblongata from normal cats, scattered GAL-IR cell bodies were encountered within the nucleus raphe obscurus and nucleus raphe pallidus. Electron microscopic observations revealed that the fine structure of the GAL-IR axonal boutons in the motor nucleus was similar to that of 5-HT-IR boutons with a varying number of immunoreactive large dense core vesicles. The postsynaptic element in all cases studied was a dendrite. A dense GAL-IR axonal plexus was found in the superficial laminae I-II of the dorsal horn. Coexistence was found between the GAL- and substance P-LI in fibers within the dorsal horn plexus. Spinal cord transection did not alter the pattern of GAL-LI in the dorsal horn, while the vast majority of GAL-IR axonal swellings disappeared following dorsal root sectioning. Electron microscopic observations in lamina II (substantia gelatinosa) revealed that the GAL-IR axonal terminals could be divided into two main groups. One with small to medium-sized axonal boutons formed synaptic contacts with both dendritic and axonal profiles. The other formed the central axon terminals of glomeruli, suggesting that GAL-LI may be present in C-type primary afferents. Numerous small GAL-IR cell bodies were encountered in laminae II and III. GAL-IR cell bodies were also observed in lamina X. The dorsal root ganglia contained a low but consistent number of small to medium-sized GAL-IR cell bodies, which all contained immunoreactive calcitonin gene-related peptide (CGRP). Following peripheral sciatic nerve transection, the number and the labeling intensity of GAL-IR cell bodies in the corresponding dorsal root ganglia showed a moderate increase. Radioimmunoassay revealed that the concentration of GAL-LI increased along the rostrocaudal axis of the normal spinal cord, and was about three times higher in the dorsal than in the ventral regions. The concentration in the dorsal root ganglia was intermediate to those seen in the corresponding dorsal and ventral cord regions.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1990

Enkephalin-like immunoreactivity levels increase in the motor nucleus after an intramedullar axotomy of motoneurons in the adult cat spinal cord.

Neuropeptide- and serotonin-like immunoreactivities were studied in the lumbar spinal cord of the cat after a longitudinal incision in the ventral funiculus. This lesion, which accomplishes a central axotomy of motoneurons, was accompanied by increased level of Met-enkephalin-like immunoreactivity in nerve endings in those parts of the motor nucleus (lamina IX) which harbored severed motoneurons. This increase, which was evident in the fluorescence microscope, could be verified photometrically by use of a confocal scanning laser microscope.

Animals↗
PubMed1990

Evidence for coexistence between calcitonin gene-related peptide and serotonin in the bulbospinal pathway in the monkey.

By the use of the indirect immunofluorescence and in situ hybridization techniques, the distribution of calcitonin gene-related peptide (CGRP)-like immunoreactivity (LI) and CGRP mRNA was studied in the spinal cord as well as in the midline raphe nuclei and the hypoglossal nucleus in the medulla oblongata of the monkey (Macaca fascicularis). In the spinal cord only a few large neurons in the motor nucleus contained CGRP-LI, while a majority of the neurons in the hypoglossal nucleus contained CGRP-LI. A relatively dense innervation by CGRP-immunoreactive (IR) fibers was also seen close to cell bodies and proximal dendrites of large neurons in the motor nucleus, especially in its ventral part. 5-Hydroxytryptamine (5-HT)-, substance P- and thyrotropin-releasing hormone (TRH)-IR varicosities were also observed in a similar position around large neurons in the motor nucleus. Double labeling disclosed that the majority of CGRP-IR axon terminals also contained 5-HT-LI. Expression of CGRP mRNA was found in neurons in the medullary midline raphe nuclei and in large neurons in the motor nucleus at the cervical spinal cord level. In adjacent sections of the medulla oblongata, CGRP-labeled neurons in the midline raphe nuclei also expressed preprotachykinin mRNA. The present results show that CGRP- and 5-HT-LI coexist in fibers within the motor nucleus of the monkey spinal cord and that this coexistence is probably due to the presence of CGRP in the descending bulbospinal, serotonergic pathway.

Animals↗
PubMed1990

5-hydroxytryptamine immunoreactive varicosities in the lamprey spinal cord have no synaptic specializations--an ultrastructural study.

The distribution and fine structure of 5-hydroxytryptamine (5-HT) immunoreactive cell bodies and axonal varicosities have been studied in the lamprey spinal cord, using the peroxidase-antiperoxidase (PAP) immunohistochemical technique and subsequent analysis of ultrathin serial sections. Immunostained cell bodies were found in the ventral spinal cord close to the central canal. Immunostained varicosities were found throughout the spinal cord with the highest density in the ventromedial plexus and the dorsal horn. Only large granular vesicles could be clearly distinguished in immunostained cell bodies and varicosities, but it was concluded based on a comparison with unstained normal tissue that these boutons also contained small, pleomorphic agranular vesicles. Immunoreactive varicosities were studied in the ventromedial plexus, the dorsal horn, the dorsal column, the dorsolateral and ventrolateral funiculi and the grey matter. No morphological differences could be observed between varicosities in the different loci. The varicosities were in no case seen to make synaptic contact with surrounding neuronal elements, even when followed through serial sections. Consequently, 5-HT released from boutons in all parts of the spinal cord could be expected to act on 5-HT receptors located on nearby as well as distant receptors.

Animals↗
PubMed1990

5-Hydroxytryptamine, substance P, and thyrotropin-releasing hormone in the adult cat spinal cord segment L7: immunohistochemical and chemical studies.

The terminal projections of the descending 5-hydroxytryptamine (5-HT) bulbospinal pathway and the coexistence among 5-HT-, substance P (SP)-, and thyrotropin-releasing hormone (TRH)-like immunoreactivities (LI) in fibers innervating the L7 segment in the cat spinal cord were studied quantitatively and semiquantitatively by use of the indirect double-staining immunofluorescence technique. The content of 5-HT, SP, and TRH in different parts of the spinal cord was determined by use of radioimmunoassay (RIA) (SP and TRH) and high-performance liquid chromatography with electrochemical detection (HPLC-ECD) (5-HT). For all three substances studied, immunoreactive (IR) axon terminals were found in all parts of the gray matter, but with clear regional variation in the density of innervation. Thus, all three substances showed a dense innervation in the motor nucleus, particularly in the ventral part of the nucleus, while the superficial dorsal horn was very densely innervated by SP-IR fibers (laminae I and II) and TRH-IR fibers (laminae II and III). In the motor nucleus, the studied substances coexisted to a very high degree, but some 5-HT-IR fibers (about 10%) lacked peptide-LI and some SP-IR fibers (about 10%) lacked 5-HT-LI while virtually all TRH-IR fibers also contained 5-HT-LI. In the superficial dorsal horn (laminae I-III), no coexistence was detected, while other parts of the gray matter displayed various degrees of coexistence in between those found in the motor nucleus and laminae I-III. The quantitative analysis of IR varicosities in the motor nucleus suggested that the unilateral L7 motor nucleus is innervated by about 55-110 x 10(6) 5-HT-IR nerve terminals, which may indicate as many as 4,000 boutons per descending 5-HT cell body in the brain stem only with this restricted projection. When combing these results with the biochemical data, it could be calculated that the concentration of 5-HT in IR varicosities is about 3-6 x 10(-3) M, while the corresponding figures for SP and TRH was 0.3-0.5 x 10(-3) M and 0.1-0.2 x 10(-3) M, respectively. In cats subjected to spinal cord transection at the lower thoracic level, all 5-HT-IR fibers in the L7 segment had disappeared 44 days after the lesion, indicating a strict suprasegmental origin of 5-HT-IR fibers in this segment.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1990

Peripheral nerve section induces increased levels of calcitonin gene-related peptide (CGRP)-like immunoreactivity in axotomized motoneurons.

By use of fluorescence immunohistochemistry it is shown that sciatic nerve section in cat and rat induces increased levels of immunoreactive calcitonin gene-related peptide (CGRP) in axotomized motoneurons. In the rat, this effect was clearly seen at 2-5 days postoperatively, but could not be demonstrated after 11-21 days. These findings are discussed in relation to previously proposed roles for CGRP in motoneurons.

Animals↗
PubMed1989

A persistent defect in the blood-brain barrier after ventral funiculus lesion in adult cats: implications for CNS regeneration?

Previous studies have shown that, in adult cats, spinal motoneurons are able to regenerate their axons after lesions in the ventral funiculus of the spinal cord. These axons regrow through a scar tissue composed of glial processes and connective tissue elements before they enter the denervated ventral root. In the present study the integrity of the blood-brain barrier (BBB) in the lesion area was assessed by i.v. injections of horseradish peroxidase (HRP), 3 weeks to 7 months postoperatively. The lesion area in the lumbosacral spinal cord was compared with the intact cervical spinal cord and the area postrema in the light and electron microscope. The results show that the BBB fails after a ventral funiculus lesion. The BBB was not restored during the examined period. The leakage of HRP appears to be the result of a transendothelial vesicular transport. In addition, it was observed that the blood vessels in the lesion area were surrounded by wide and irregular perivascular spaces with broken outer basal laminae. Other studies on traumatic defects in the BBB have indicated that the barrier is reorganized within 4 weeks after the lesion. The possibility that a prolonged defect in the BBB after a ventral spinal cord lesion might be linked with the survival of axonal sprouts is discussed.

Animals↗
PubMed1989

Altered levels of calcitonin gene-related peptide (CGRP)-like immunoreactivity of cat lumbar motoneurons after chronic spinal cord transection.

In cats subjected to total spinal cord transection at the lower thoracic level, the calcitonin gene-related peptide (CGRP)-like immunoreactivity (LI) was studied in motoneuron cell bodies in the L6-L7 segments. In transected animals, the CGRP-immunoreactive labeling of the motoneurons was virtually absent. When combining the spinal cord transection with a unilateral rhizotomy of all dorsal roots below the transection, however, an apparently normal labelling pattern of CGRP-LI of the motoneurons was displayed on both sides. Thus, surgical interventions which affect afferent pathways to the motoneurons may have influence on the levels of CGRP-LI in otherwise intact motoneurons.

Animals↗
PubMed1989

Motoneurons reinnervate skeletal muscle after ventral root implantation into the spinal cord of the cat.

By use of intracellular recording and staining with horseradish peroxidase it was found that alpha and probably also gamma motoneurons were able to reinnervate ventral root implants after an avulsion of ventral roots at the spinal cord surface in the cat. The reinnervation of the implant was achieved after an initial growth of new axons in central nervous system tissue. Reinnervating neurons could be excited or inhibited by segmental reflex activity and their axons could conduct nerve impulses. The character of muscle twitch responses elicited by electrical stimulation of implanted roots strongly indicated that denervated muscles were reinnervated by new motor axons via the implant.

Action Potentials↗
PubMed1989

Nerve fibre regeneration across the PNS-CNS interface at the root-spinal cord junction.

Root-spinal cord regeneration was investigated in immature and adult rats. The elongation in the dorsal root of regrowing dorsal root axons, rerouted ventral root nerve fibres (cholinergic neurons) or hypogastric nerve fibres (catecholaminergic neurons) is impeded as they meet the astrocyte dominated CNS tissue of the root. The establishment of synaptoid nerve terminals as the regrowing axons encounter astrocytes indicates a mechanism for growth inhibition other than a physical impediment in the CNS environment. The glial cells of the CNS segment in the root are influenced by the type of regenerating nerve fibres in terms of maintenance, multiplication and phenotypic expression. After a dorsal root lesion in the neonatal rat several root axons may reinnervate the spinal cord. In these rats, the normal establishment of a CNS root segment has been disrupted and the PNS-CNS border is situated central to the root-spinal cord junction. Implantation of cut dorsal roots into the spinal cord of adult rats results in the extension of processes from intrinsic spinal cord neurons out into the root. After implantation of avulsed ventral roots into the ventro-lateral aspect of the cord, axonal regrowth and functional restitution of alpha-motoneurons could be demonstrated by intracellular recordings and injections with horseradish peroxidase. These results show that regeneration can occur across a PNS-CNS interface that has been established secondary to a trauma in the mature animal and in the immature animal before the astrocyte-rich CNS root segment has been developed.

Age Factors↗
PubMed1989

Distribution of TRH-like immunoreactivity with special reference to coexistence with other neuroactive compounds.

During the last years, several important advancements have been made that are of importance for our understanding of the distribution and localization of neurons and cells producing TRH-LI. As detailed in other chapters in this volume, the precursor for TRH has been characterized that has allowed production of antibodies raised against specific sequences of this precursor. This, in turn, has provided new tools for the immunohistochemical elucidation of TRH systems in the CNS. The TRH precursor has also been cloned, leading to possibilities for studying the localization of TRH mRNA with in situ hybridization. Finally, as shown in this paper, improvement of the fixation technique has made it possible to visualize extensive TRH-immunoreactive cell body and fiber systems with antiserum raised against the TRH tripeptide. The results from the latter studies and those with antisera directed to the TRH precursor and in situ hybridization are in good agreement, with some minor exceptions. It should be pointed out that some of the systems described here, for example TRH positive-cell bodies in cortical areas and the hippocampal formation, contain only a very weak immunoreactivity. As always with immunohistochemical techniques, the possibility of crossreactivity with TRH-like peptides or TRH-like sequences within larger proteins must be considered. The present results confirm the presence of TRH-LI in the insulin-producing beta cells of the pancreas, which with the improved technique can be demonstrated also in early adulthood in rats and guinea pigs. Moreover, it could be established that TRH-LI is present in neurons in the gastrointestinal tract as well as in a population of endocrine cells in the antrum of the stomach of the guinea pig. These cells seem at least partly to be identical to the well-known gastrin-producing cells. TRH-LI has been observed to occur in neurons already containing a classical transmitter and/or other peptides. Of particular importance here seems to be a descending bulbospinal system that in addition to TRH co-contains 5-HT, substance P-LI, galanin-LI, human growth hormone immunoreactive material, and proctolin-like material. The significance of this coexistence is not well understood, but interesting interactions have been observed. Attempts to manipulate the TRH phenotype in these medullary neurons by transplantation to other sites in the brain has so far shown that the expression of this peptide seems fairly stable.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1989

Identification of excitatory interneurons contributing to generation of locomotion in lamprey: structure, pharmacology, and function.

1. In the in vitro preparation of the lamprey spinal cord, paired intracellular recordings of membrane potential were used to identify interneurons producing excitatory postsynaptic potentials (EPSPs) on myotomal motoneurons. 2. Seventy-nine interneurons (8.4% of all neuron-motoneuron pairs tested) elicited unitary EPSPs that followed one-for-one at short, constant latencies and were therefore considered monosynaptic according to conventional criteria. Evidence was obtained for selectivity and divergence of excitatory interneuron (EIN) outputs and for convergence of EIN input to motoneurons. 3. The neurotransmitter released by EINs may be an excitatory amino acid such as glutamate, because the EPSPs were depressed by antagonists of excitatory amino acids. 4. Intracellular dye injection revealed that EINs have small cell bodies (average 11 x 27 microns), transversely oriented dendrites, and thin (less than 3 microns) slowly conducting axons (0.7 m/s) that project caudally and ipsilaterally. One EIN exhibited a system of thin multi-branching axon collaterals with periodic swellings. Ultrastructurally, these swellings contained clear spherical vesicles, and they apposed postsynaptic membrane specializations. 5. During fictive locomotion, the membrane-potential oscillations of EINs were greater in amplitude than, but similar in shape and timing to, those of their postsynaptic motoneurons. EINs fired action potentials during fictive locomotion and contributed to the depolarization of motoneurons. 6. These interneurons are proposed to be a source of excitation to motoneurons and interneurons in the lamprey spinal cord, participating in motor activity including locomotion.

Action Potentials↗
PubMed1988

Postnatal development of cat hind limb motoneurons. I: Changes in length, branching structure, and spatial distribution of dendrites of cat triceps surae motoneurons.

The postnatal development of length, branching structure, and spatial distribution of dendrites of triceps surae motoneurons, intracellularly stained with horseradish peroxidase, was studied from birth up to 44-46 days of postnatal (d.p.n.) age in kittens and compared with corresponding data from adult cats. The number of dendrites of a triceps surae motoneuron was about 12, and the arborization of each dendrite generated an average of 12-15 terminal branches. There was no net change in the number of dendrites of a neuron or in the degree of branching of the dendrites despite the occurrence of both a transient remodeling of the dendritic branching structure and changes of the spatial distribution of the dendritic branches during postnatal development. The perisomatic territory in the transverse plane occupied by the dendritic branches of a motoneuron increased in parallel with the overall growth of the spinal cord. Thus, the relative size of the dendritic territory in this plane was kept almost constant, whereas dendritic branches projecting in the rostrocaudal direction grew much faster than the spinal cord and also became more numerous. At birth the rostro-caudal dendritic span of individual motoneurons bridged 1:6 to 1:5 of the L7 spinal cord segment length; this figure was 1:3 at 22-24 d.p.n. Hence, in this direction, the growing dendritic branches invaded novel dendritic territories. The change in dendritic branch length from birth to 6 weeks of age corresponded to an average growth rate of 2 to 4 microns per dendritic branch and day, which implies that the total increase in length of the dendrites of a neuron could amount to 1 mm/day. The increase in branch length did not occur in a uniform or random manner; instead, it followed a spatiotemporal pattern with three phases: From birth to 22-24 d.p.n., growth was particularly prominent in greater than or equal to 3rd order preterminal and 2nd through 6th order terminal branches. From 22-24 to 44-46 d.p.n., a large increase in branch length confined to terminal branches of greater than or equal to 3rd branch orders was observed. As indicated by topological analysis, this length increase was probably due in part to a resorption of peripheral dendritic branches during this stage of development. From 44-46 d.p.n. to maturity, the increase of dendritic branch length was restricted to preterminal branches of low (less than or equal to 4th) branch order.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1988

Postnatal development of cat hind limb motoneurons. II: In vivo morphology of dendritic growth cones and the maturation of dendrite morphology.

The maturation of dendrite morphology was studied by light and electron microscopy in cat spinal alpha-motoneurons intracellularly labeled with horseradish peroxidase. Alpha-motoneurons supplying the triceps surae (TS) and the intrinsic foot sole (SP) muscles were investigated in kittens from birth to 44-46 days of postnatal (d.p.n.) age. At birth, a large number of dendritic branches displayed growth cones, filopodia, and fusiform processes. The growth cones were of lamellipodial and filopodial types, but intermediate forms also occurred. The growth cones shared several morphological features with the neuritic growth cones studied in vitro. It was suggested that the occurrence of different types of growth cones--even in the same dendrite--may reflect their transformation from one type to the other and the level of growth activity could be inferred from the number and form of the growth cones. About 50-70% of the terminal branches in the dendrites of newborn kittens possessed growth cones, filopodia, and/or fusiform processes. The corresponding figure for preterminal branches was 20-30%, with a clear decrease in incidence when approaching the soma. During the period under study, most of these growth-associated processes disappeared from the dendrites so that at 44-46 d.p.n. of age only about 10% of the terminal and less than 1% of the preterminal branches had growth-associated processes. Analysis of the three-dimensional distribution of dendritic branches with such processes disclosed that they were relatively more frequent in the medial, rostral, and caudal dendritic territories. It was concluded that the pattern of distribution and disappearance of growth cones, filopodia, and fusiform processes coincided with postnatal longitudinal dendritic growth and the development of the adult dendritic territories described in a preceding paper (Ulfhake et al., '88). Dendritic growth, with respect to length and caliber, also occurred in the absence of growth cones and filopodia. It is suggested that the important role of these processes may be to act as a steering device in establishing the adult distribution and synaptology of the dendrites. Comparison of TS and SP alpha-motoneuron dendrite morphology at birth and at 22-24 d.p.n. age showed that the SP neurons lagged in the maturation process. Light and electron microscopic observations indicated that postnatally direct contacts might exist between dendrites and fine blood vessels in the neuropil without any interposing glial sheath. The number of such suspected contacts diminished during the period under study, indicating that the glial ensheathment of the blood vessel takes place, in part, postnatally.

Animals↗
PubMed1988

Postnatal development of cat hind limb motoneurons. III: Changes in size of motoneurons supplying the triceps surae muscle.

The postnatal changes of neuronal dimensions were studied in cat triceps surae motoneurons intracellularly labeled with horseradish peroxidase. Systematic correlations were observed in the analysis of single dendrites at each studied stage, from birth to 44-46 days post natum (d.p.n.) age, between size parameters intrinsic to the dendrites as the diameter of a 1st-order dendrite, the combined dendritic length, the dendritic membrane area, and the degree of branching. Some variability among samples was evident in each studied age group. The correlations were, however, sufficiently close to permit indirect estimations of both combined dendritic length and dendritic membrane area for larger samples of neurons from data on dendritic stem caliber. The total postnatal increase in dendritic membrane area was, on the average, 400%, i.e., from close to 100 X 10(3) microns2 to about 500 X 10(3) microns2. The corresponding increase in soma area amounted to 100%. Analysis revealed that there was a time lag between the increase in somatic and dendritic size. Thus, adult somatic dimensions were attained at age 44-46 d.p.n.; however, at this stage, the mean total dendritic membrane area was only about half of the adult value. The postnatal increase in size appeared to vary among neurons, yielding a wider neuronal size spectrum in the adult cat than that observed in kittens. The measured increase in size corresponded to a calculated average addition of dendritic membrane area of 3700 microns2/day from birth to 22-24 d.p.n. and from that stage to 44-46 d.p.n. of 2700 microns2 per day. Likewise, the increase in combined dendritic length could initially be as large as 1 mm/day down to 0.4 mm/day between 22-24 and 44-46 d.p.n., with a mean growth during the first 44-46 d.p.n. of 0.5 to 0.6 mm/day. The ratios of daughters to parent branch diameters (sigmadd1.5: dp1.5) and the dendritic trunk parameter (sigma d1.5) recorded along the proximodistal dendritic path distance revealed transient changes that might impact on the electrotonic properties of the dendrites during postnatal development. Computations from the measured changes in dendritic branch lengths and calibers indicated that if membrane and internal resistivity remain unaltered during postnatal development, the dendritic domain is electrotonically more compact in the newborn kitten than in the adult cat.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1987

An ultrastructural study of the synaptology of gamma-motoneurones during the postnatal development in the cat.

The postnatal development of cat triceps surae gamma-motoneurones, retrogradely labelled with horseradish peroxidase (HRP), was studied light and electron microscopically. The mean diameter of the cell bodies of the gamma-motoneurones increased by about 25% from birth to the adult stage, which was much less than the increase in cell body diameter of alpha-motoneurones (about 45%). Throughout development the only bouton types apposing the gamma-motoneurones were the F- and S-types, with flattened and spherical synaptic vesicles, respectively. Thus, the C-, M- and T-types of boutons seen on a alpha-motoneurones. The number of boutons on the gamma-motoneurone cell bodies seemed to decrease postnatally. This decrease was only moderate for S-type boutons but substantial for F-type boutons. In contrast, the number of boutons on the proximal dendrites appeared to increase and this was most evident for S-type boutons. The mentioned postnatal changes in synaptology were more differentiated with regard to bouton type and part of the neurones under study than what could be inferred from earlier studies on the postnatal development of alpha-motoneurones. These changes also occurred later than in alpha-motoneurones. The relative dominance of F-type boutons with probable inhibitory actions on the immature gamma-motoneurone may explain the previously demonstrated poor encoding of muscle length by muscle spindles during the first postnatal weeks in the kitten.

Aging↗