PubMed Health⌕ Search

Biomedical subjects

S D Puthucheary

Publications and source records attributed to S D Puthucheary.

At least 73 records · Page 4Linked to original sources
PubMed1988

Melioidosis, the great mimicker: a report of 10 cases from Malaysia.

Between 1981 and 1986, 10 consecutive cases of melioidosis were seen at the University Hospital, Kuala Lumpur, Malaysia. They illustrate the amazing guises of melioidosis presenting as: abscesses of the supraspinatus muscle, psoas muscle, brain and liver; three different pulmonary forms; an acute suppurative dermal lesion; an acute septicaemia; and chronic lymphadenitis. The majority had underlying diseases: diabetes mellitus, the commonest, was present in six, out of whom three had previous pulmonary tuberculosis; other predisposing conditions were renal failure, corticosteroid therapy and malnutrition. Three patients who died had pre-existing renal impairment and developed renal failure later, suggesting that the former is a bad prognostic sign. Clinical diagnosis was difficult: all cases were diagnosed bacteriologically. A high level of clinical awareness is necessary, especially when presentation simulates pulmonary or extrapulmonary tuberculosis in patients with diabetes or other compromised states.

Abscess↗
PubMed1987

In-vitro and in-vivo studies of a cytotoxin from Campylobacter jejuni.

Studies were performed on a cytotoxin (CT) from human strains of Campylobacter jejuni isolated in Malaysia. CT was detected by cytopathic effect (CPE) on HeLa cells at titres from 8 to 32, in culture filtrates from 14 (48%) of 29 human isolates. The CPE correlated well with a quantitative 51Cr-release assay where a specific release of 54-68% was noted. CT production was lost after 5-7 subcultures. CT activity was also detected in 5 (26%) of 19 faecal filtrates from which CT-producing isolates were subsequently obtained. The mol. wt of CT was estimated by Sephadex G-50 chromatography to be greater than 30,000. In a suckling-mouse assay, CT consistently failed to demonstrate fluid accumulation after intragastric inoculation of culture filtrate. The Removable Intestinal Tie Adult Rabbit Diarrhoea (RITARD) assay was also used. Rabbits given CT-producing strains of C. jejuni developed bacteraemia and severe watery mucus-containing diarrhoea for the duration of the experiment with death of some animals. Rabbits given CT non-producing strains had less severe disease and none died. Rabbits given partially-purified CT had diarrhoea for 3 days but none died.

Animals↗
PubMed1987

Distribution of group B streptococcal serotypes in Malaysia.

A total of 350 strains of group B streptococci (308 from adults and 42 from neonates) were serotyped. The majority of the isolates from adult patients were from the genito-urinary tract and most of the neonatal isolates were from babies less than 10 days old. Serotype III was the predominant type among all groups of patients and accounted for 50.9% of all serotypes. The second and third most frequently occurring serotypes were II and Ic respectively. Together with type III they made up 87% of the isolates.

Adult↗
PubMed1983

The prevention of postappendicectomy sepsis by metronidazole and cotrimoxazole: a controlled double blind trial.

A double-blind, randomized controlled trial was carried out in University Hospital, Kuala Lumpur to study the effect of metronidazole and cotrimoxazole on the incidence of wound infection following appendicectomy from November 1978 to January 1980. Patients were allocated at random into one of four groups: cotrimoxazole injection and placebo suppository, metronidazole suppository and cotrimoxazole injection, metronidazole suppository and placebo injection or placebo suppository and placebo injection. Treatment was started 30 min before operation and continued for 72h. All patients were followed up for 2 weeks and thereafter for one month. A total of 283 patients was finally accepted into the study. Sepsis rates were found to be 27% for the untreated group, 9% for the group receiving metronidazole only, 8% for the group receiving cotrimoxazole injection only and 2.7% for the group receiving both drugs. The study showed that a combination of metronidazole and cotrimoxazole is a regime highly effective for prophylaxis against wound infection following appendicectomy.

Appendectomy↗
PubMed1983

Comparison of two methods for bacteriocin typing of Serratia marcescens.

Two methods of bacteriocin susceptibility typing for Serratia marcescens were compared. A total of 80 epidemiologically unrelated isolates from patients in a single hospital were typed by the cross-streaking method and the mitomycin C-induced (spotting) method. The cross-streaking method was found to be more discriminatory than the spotting method. Using the cross-streaking method, it was possible to differentiate 50 bacteriocin groups out of the 80 isolates, whereas only 31 groups could be obtained with the spotting method. The reproducibility and percentage typability of the cross-streaking method (82.5 and 93.75%, respectively) were found to be as good as, if not better than, those of the spotting method (78.75 and 90.0%, respectively). Other factors, such as lower economic cost, technical simplicity, and the relative ease in the scoring of results, indicate a preference for the cross-streaking method. The findings of this study support the choice of the cross-streaking method for the bacteriocin typing of S. marcescens in epidemiological studies.

Bacteriocins↗
PubMed1983

Demonstration of a cytotoxin from Campylobacter jejuni.

A 48-hour culture filtrate of Campylobacter jejuni was found to produce cytopathic effects on three human cell lines--that is, HeLa, MRC-5 and Hep-2. The cytopathic effects observed include cell rounding, loss of adherence and cell death after 24-48 h of incubation. Such morphological changes were observed with eight of the eleven strains of Campylobacter jejuni isolated from the blood/stools of patients who suffered from either acute gastroenteritis or septicaemia. The toxic factor did not retain its activity after treatment at 100 degrees C for 30 min. Trypsinisation of the filtrate totally abolished its toxic activity thus indicating that it was probably protein in nature. It is most probably an extracellular toxin as bacterial sonicates did not produce any toxic effect. This study reports the finding of toxic factor(s) in the culture filtrate of Campylobacter jejuni which is cytotoxic to human tissue culture cells.

Adult↗