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Biomedical subjects

S Deng

Publications and source records attributed to S Deng.

At least 91 records · Page 5Linked to original sources
PubMed1996

Combined islet-lung transplantation in a cystic fibrosis patient.

The prevalence of insulin-dependent diabetes mellitus (IDDM) in cystic fibrosis patients ranges from 2 to 8% and glucose intolerance up to 15%. In recent years, lung transplantation has helped to prolong life expectancy of cystic fibrosis patients and represents 10 to 30% of all indications for lung transplantation. The postoperative need for immunosuppressive therapy using diabetogenic agents has decompensatory effects on glucose regulation and will probably increase the number of insulin-dependent cystic fibrosis patients. We report the case of an insulin-dependent cystic fibrosis patient transplanted with a combined islet-lung allograft. The pre-transplantation C-peptide level was below 0.04 nmol/l and preoperative insulin requirement was some 100 U per day. A sequential bipulmonary lung transplantation was performed and, using the pancreas of the same donor, we isolated and purified the islets of Langerhans by a modified automated method. We obtained 232,200 islets equivalent, which were injected into the liver by portal embolization. The postoperative course was uncomplicated, the insulin requirement decreased to 50% of the preoperative need and the C-peptide value increased to normal values and remained with the normal range during a follow-up period of 15 months. In conclusion, we believe that, besides type I diabetic patients, insulin-dependent cystic fibrosis patients with a negative C-peptide value could also be good candidates for combined islet-lung allotransplantation.

Adult↗
PubMed1996

[A preliminary study of the preventive and blocking effect of green tea grown in mengding mountain area on the esophageal cancer in rats].

Three groups (positive control or nitrosamine alone group, test group and control group) were included in this experiment. After the begining of the experiment, the rats of test group were given 2% green tea (common grade) solution by free drinking daily for 24 weeks. After 2 weeks of the begining of the experiment, the rats of the nitrosamine alone group and test group were injected with MANA, 5 mg/kg weekly for 20 weeks. The animals were sacrificed at 4 intervals. The incidence rates of esophageal cancer in the rats of the two groups sacrified at every interval were significantly different. The result suggested that green tea should have a preventive and blocking effect on the occurrence of esophageal cancer in rats.

Animals↗
PubMed1996

[Autotransfusion of shed mediastinal blood after open heart operation].

This prospective study was designed to determine whether the autotransfusion of shed mediastinal blood (ATS) after open heart surgery is safe and effective. Forty-two patients undergoing cardiac operation were randomized to receive either nonwashed shed mediastinal blood (group 1; n = 22) or banked blood (group 2: n = 20). No difference in mean age (group 1: 49 +/- 11 years; group 2: 45 +/- 12 years), coronary artery bypass grafting (group 1: n = 5, 23%; group 2: n = 6, 30%), valve replacement (group 1: n = 17, 77%, group 2: n = 14, 70%), and mean preoperative hemoglobin level (group 1: 13.7 +/- 2.3, group 2: 14.4 +/- 1.6) was noted between non-ATS and ATS groups (p = not significant). The mean hemoglobin levels after operation were similar in the two groups (group 1: 11.89 +/- 1.52; group 2: 12.03 +/- 1.34). No difference in the mean blood loss 4, 6 and 24 hours after operation (group 1: 33 +/- 190, 420 +/- 340 and 550 +/- 300; group 2: 340 +/- 230, 420 +/- 280 and 670 +/- 380) was observed between the two groups. The mean volume autotransfused in group I was 380 +/- 230 ml (200 approximately 1300 ml). In group I, the patients required bank blood 1080 +/- 720, compared with 1780 +/- 1045 in group II. The bank blood requirement in group I reducted by 40%. These data demonstrate that ATS after open heart surgery is safe and effective.

Adult↗
PubMed1995

[The effects of mupirocin on burned wound with Staphylococcus aureus infection].

The therapeutic results of 41 cases of S. aureus infected burn wound (average 2.1% TBSA, approximately 200 cm2) treated with Mupirocin are reported in this paper. The effects of Mupirocin was obviously superior to that of SD-Ag used in control group. During the observation period the total effective rate of the studied group was 88.9% (control group: 70%, P < 0.05) and the bacterial clearance rate of S. aureus from the wound was 88.8% (control group: 40% P < 0.05). The sensitivity of 30 strains of S. aureus isolated from burn wounds to 11 varieties of antibiotics indicated that the sensitive rate to Mupirocin was as high as 92.68%, only lower that Vanconmycin. The bacteriological assay (MIC < or = 0.25 mg/L, MIC < or = 4 mg/L) also showed high sensitivity of S. aureus to Mupirocin. We suggest Mupirocin be the first choice of topical antibacterial agents for burn wound with S. aureus infection, especially for infection with MRSA.

Burns↗
PubMed1995

[Application of gasless laparoscopic device in laparoscopic surgery].

The aim of this study was to evaluate the gasless laparoscopic device (GLD) in laparoscopic surgery. GLD, independently designed and manufactured by ourselves in 1993, incorporates a manipulator and laparoscopic abdominal retractor. From August to October 1993, we conducted an experimental study on gasless laparoscopic cholecysto-duodenostomy in 5 dogs. Four of them were completely successful and one was converted to open surgery due to gallbladder rupture. The result showed that GLD could maintain good exposure in laparoscopic surgery, laparoscopic suturing and knotting could become easier and operative cost could be reduced with conventional instruments. We also developed an intracorporeal "Chinese Knotting" and a self-designed push tube for extracorporeal knot tying. During April and August 1994, we successfully applied our modified GLD in gasless laparoscopic cholecystectomy, inguinal herniorrhaphy laparoscopic-assisted sigmoidectomy, abdomino-perineal resection of rectal carcinoma.

Adenocarcinoma↗
PubMed1995

[Changes in serum granulocyte colony-stimulating factor (G-CSF) in burned patients].

Serum G-CSF level was assayed in 20 burned patients (TBSA > or = 30%) 2 weeks postburn. In 11 of them plasma endotoxin was also measured. The results showed serum G-CSF level was increased in 87.5% (7/8) of burned patients with exceeding 50% TBSA and in 58.3% (7/12) of patients with 30%-50% TBSA, and in the former it was earlier increased (1-5 day postburn) than the latter (5-11 day postburn). Serum G-CSF was increased in 90% of the burned patients with wound sepsis. Increased of serum G-CSF in burned patients, especially in those exceeding 50% TBSA, indicates the occurrence of sepsis.

Burns↗
PubMed1995

[A preliminary observation of preventive and blocking effect of Gynostemma pentaphyllum (Thunb) Makino on esophageal cancer in rats].

Gynostemma pentaphyllum (Thunb) Makino is a kind of natural plant growing in the southern provinces of China. In this experiment, rats were given drinks of 2% Gynostemma Pentaphyllum (Thunb) makino aqua boiled solution freely for 2 weeks before administration of carcinogen. Subsequently, the rats were injected with carcinogen (MANA) for 18 weeks and were sacrificed at intervals. The result showed that the number of cases of esophageal epithelial papilloma, the average number of tumor and the incidence of esophageal cancer in the experimental group were lower than those in the control group (MANA only), though no statistical significant difference was found. The initiation of cancer was delayed for 6 weeks. These indicated that Gynostemma Pentaphllum Makino might have some preventive and blocking effect on esophageal cancer in rats.

Animals↗
PubMed1995

[Islets of Langerhans allografts in the pig: initial results].

Islet of Langerhans transplantation represents a promising treatment of diabetes. Use of porcine islets for xenotransplantation could offer a solution to shortage of organ donors. However, isolation of pig islets remains a real challenge because of their marked fragility. Using a modified automated method for islet isolation, we performed 10 intraportal islet allografts in pigs. Surgically pancreatectomized pigs were transplanted with purified islet preparations pooled from pancreases of 3 donors (slaughter-house pigs, age 5-8 months). Six recipients were not immunosuppressed and four received an immunosuppressive treatment of Cyclosporine and Azathioprine. In the first group (non-immunosuppressed recipients), insulin secretion was observed for a mean of 4.8 days after transplantation. In the second group (with immunosuppressive treatment) the recipients sustained an insulin secretion for 6-9 days. However, in both groups liver biopsies showed signs of acute rejection and destruction of the transplanted islets. Pig islet allotransplantation, using as recipients surgically pancreatectomized pigs, can be considered as a suitable model to assess the functional results of mass islet isolation. We were able to reverse diabetes transitory in a large animal, by transplantation of purified pig islet preparations. However, even when an immunosuppressive treatment was administered to the recipients, rejection seemed to represent an important factor in the functional outcome of the islet grafts.

Animals↗
PubMed1995

[Mechanism of rejection of islet of Langerhans xenografts].

For immediately vascularised xenografts, the presence of preformed antibodies associated with the activation of the complement induces a hyperacute humoral rejection in discordant combinations. For neovascularised xenografts, such as the islets of Langerhans, the absence of an initial vascularisation prevents the acute humoral rejection and there is some evidence to suggest that the rejection is cell mediated. To analyse the influence of species response to cellular transplantation, we compared the functional outcome and the immunohistochemical analysis of concordant and discordant xenotransplated islets with allotransplanted islets of Langerhans. In the concordant xenotransplantation experiment, rat (Sprague Dawley) purified islets (N = 600) were transplanted under the kidney capsule of streptozotocin diabetic mice. In the discordant experiment, purified human islets (N = 1000), in the allotransplantation model Balb mouse islets (N = 600) were transplanted under the kidney capsule of C57BL/6 mice. Daily blood glucose levels were monitored until rejection occurred (blood glucose > 11 mMol/L) and kidney biopsies were taken every second day for immunohistological examination. In allotransplanted mice, rejection occurred after a mean of 15 +/- 4 days, in concordant xenotransplanted mice after a mean of 10 +/- 3 days and in the discordant xenotransplanted mice after a mean of 8 +/- 1 days. The immunohistological analysis showed in both xenotransplantation models a deposit of IgM and C3 surrounding the grafted cells, starting at day 1 and persisting until rejection, associated with the presence of Mac-1 positive cells. The intensity of the humoral response was moderate to important in the concordant group, but very strong and unchanged until rejection in the disconcordant group. An invasion with CD4+ and CD8+ cells started between day 4 and 6 in both groups, progressively increasing until rejection. In the allotransplantation model, no humoral response was detectable and the cellular response started between day 10 and 12 after transplantation. We conclude, that a cellular response is involved in the rejection process of both concordant and discordant xenotransplanted islets of Langerhans. Compared to an allotransplantation model, the humoral response does not destroy the function of transplanted islets, but could trigger or accelerate the cell-mediated response.

Animals↗
PubMed1994

[Studies on specific diagnostic antigen for filariasis].

Soluble antigens of adult worms (MAA) and microfilariae (MFA) were analysed by means of SDS-PAGE and enzyme-linked immunoblotting (ELIB). The adult worms and microfilariae were collected from abdominal cavity of Mongolian jirds infected with Brugia malayi. The results indicated that MFA shared 64-67 kDa and 56-58 kDa protein components with the peritoneal fluid of the normal jirds. Sera from normal jirds were found to recognize 60 kDa, 74 kDa and 100 kDa fractions of MFA, but did not recognize any bands of MAA. Sera from positive jirds which were infected with B. malayi for 6 months could recognize 42 kDa and 14.5 kDa of MAA, but sera from negative jirds could not. The results indicated that the 42 kDa and 14.5 kDa protein components of MAA might be specific diagnostic antigen fractions.

Animals↗