PubMed Health⌕ Search

Biomedical subjects

S Dervisoglu

Publications and source records attributed to S Dervisoglu.

17 recordsLinked to original sources
PubMed2004

A dynamic study of the thermal components in electrical injury mechanism for better understanding and management of electric trauma: an animal model.

The thermal effects of acute electric trauma to living cells show some variation patterns and kinetics for different body components (muscle, bone, blood-vein and skin). Thermal energy transfer is random with no preferred directions, but electrical energy transfer is vectorial in the direction of the current. In the present study, a total 50 white male Wistar Albino rats, body weight 250-300 g, have been used to study electrical injury mechanism with subsequent change in muscle perfusion at different post-traumatic stages. The muscle temperature was found to increase with a sudden jump from 35.3+/-1.2 to 75.2+/-7.6 degrees C. The bone temperature increased from 35.2+/-1.4 to 45.8+/-1.2 degrees C and decayed slowly within 600+/-90 s. The venous blood curves show a similar pattern to that of muscle with a sudden jump of temperature from 36.4+/-.9 to 40.5+/-3.1 degrees C. The core temperature showed a flat pattern with a slight increase from 36.1+/-0.8 to 37.3+/-0.6 degrees C and the peak temperature was found after 70+/-10 s. From the scintigraphic study, muscle perfusion was found to be a minimum 72 h after electric shock. The right hind limbs of all the rats (ground) exhibited more intense histopathological damages (electric burns) than the left fore limb (source).

Animals↗
PubMed2004

Changes in the threshold voltage and alterations of smooth-muscle physiology after bladder outlet obstruction.

The purpose of this study was to determine the resultant changes in the bladder function after partial bladder outlet obstruction (BOO), with particular emphasis on the threshold voltage and on the role of NO. A total of 12 adult rabbits were used in the study. New Zealand-type rabbits were subjected to no intervention and 3 weeks duration of a partial outlet obstruction. They underwent an in vivo cystometric evaluation before and after the BOO, histological studies, bladder-strip stimulation studies using acetylcholine, electrical field stimulation, and relaxation studies using NO donor nitroprusside. Statistical significance was determined by two-way ANOVA for multiple variations and Student's t-test. Histology sections demonstrated smooth-muscle hypertrophy, hyperemia of the vessels in the wall and widely set mononuclear cell infiltration in the rabbits with partial BOO. Cystometry showed markedly decreased bladder capacities, and decrease of compliance from 4.3 +/- 1.8 to 1.6 +/- 0.3 in the obstructed group. Tissue bath studies demonstrated no meaningful change with cholinergic stimulation, increased contractility in response to electrical field stimulation, and increased threshold voltage values from 57.5 to 93.3 compared to controls. Nitroprusside did not induce relaxation of the neostigmine and acetylcholine-precontracted bladder in all animals. Hypertrophy was observed due to adaptation of the detrusor against the obstruction. During this adaptation period, bladder capacity decreases and the elasticity disappears, thus causing higher pressures with lesser volumes. In other words, compliance decreases. The neurogenic damage can be shown with increasing values of threshold voltage, and higher voltages are needed to start a contraction. Nitroprusside has no inhibitory effect on smooth-muscle tone in the rabbit bladder.

Acetylcholine↗
PubMed2002

Duplication of appendix vermiformis: a case in a child.

Appendiceal anomalies are extremely rare malformations that are usually found in adult population as an incidental finding during laparotomy due to another reason. When appendiceal duplications are detected in childhood, almost all the patients have serious associated intestinal, genito-urinary or vertebral malformations. Presented herein are a case of a huge (15 x 13 cm mass) appendiceal duplication in a child causing acute abdomen without an accompanying pathology, its differentiation from appendiceal and caecal diverticulum, and classification of appendiceal duplications with the review of literature.

Abdomen, Acute↗
PubMed2002

The sex hormone receptors in the bladder in childhood - I: preliminary report in male subjects.

The sex hormone receptors (oestrogen, androgen, progesterone) in the bladder were demonstrated in clinical studies by various authors in adult patients. But the presence of these receptors in childhood had not been investigated. The aim of this study was to assess the presence of sex hormone receptors in the bladder of male subjects in childhood. The study included 20 bladder biopsies from a total of 15 children. Biopsies were taken during open surgery and/or cystoscopic procedures for various indications from the bladder dome, trigonum and in the region of the bladder neck. All biopsies were routinely fixed and processed for histopathological assessment and investigated immunohistochemically to determine the sex hormone receptors in the bladder. The sex hormone receptors were demonstrated with different densities and locations. In particular, sex hormone receptors were found very frequently in biopsies taken from the bladder neck. Overall receptor positivity in the specimens was 90 % for progesterone, 65 % for androgen and 25 % for oestrogen. There was no receptor expression in the deeper tissues of the bladder wall. This preliminary study demonstrated: 1. Sex hormone receptors are present in children; 2. There was a female sex hormone (progesterone, oestrogen) receptor expression in male subjects; 3. The receptors are mainly localised at the bladder neck and in transitional epithelium of the bladder wall. We think that the sex hormone receptor map may be useful in the evaluation of lower urinary tract and specially bladder neck disorders in childhood in the future.

Biopsy↗
PubMed2001

Effects of some nonsteroidal anti-inflammatory drugs on articular cartilage of rats in an experimental model of osteoarthritis.

We evaluate the chondrotoxic effects of some nonsteroidal anti-inflammatory drugs (NSAIDs) on articular cartilage in an experimental model of osteoarthritis (OA). Each of 40 Sprague-Dawley rats weighing 0.250 kg and 12 weeks old received weekly injections of sodium iodoacetate (1 mg/0.1 ml NaCl) in the right knees for 8 weeks to induce an experimental model of OA. The left knees served as controls. Four groups were formed of ten rats each. The control group received no medicine. In the other three groups, tiaprofenic acid, diclofenac, and indomethacin were given orally for 12 weeks. At the end of the 20th week, the complete groups were sacrificed and histologic evaluation performed. In the right knees of the control group, specific morphologic changes for OA were observed. Of the three NSAIDs tested, indomethacin was found to have deleterious effects on articular cartilage of both left and right knees. Diclofenac caused a statistically significant increase in the severity of most of the osteoarthritic parameters that were examined in the right knees, whereas tiaprofenic acid was observed to have some beneficial influences on the articular cartilage of right knees. Both diclofenac and tiaprofenic acid displayed the same deleterious effects on articular cartilage of left knees. We conclude that, in the prescription of NSAIDs for OA, it would be appropriate to choose a drug with a protective effect on chondrocytes and articular cartilage.

Alkylating Agents↗
PubMed2000

Maternal cocaine abuse resulting in necrotizing enterocolitis. An experimental study in a rat model. II. Results of perfusion studies.

During the last decade, several publications have appeared associating the maternal use of cocaine and subsequent development of necrotizing enterocolitis (NEC). In 1994, the effects of cocaine in pregnant rats had been reported by this group: a significant decrease in the number of live births, mean birth weight and mean placental weight. In addition, histopathologic examinations revealed severe inflammation and degenerative vascular changes in the uterus and placenta. Severe histopathologic changes resembling NEC such as focal necrosis, necrobiosis, and hemorrhagic inflammatory changes in the gastrointestinal tract of the embryos were also reported. The aim of the second part of this study was to assess the hemodynamic effects of cocaine HCI in pregnant rats and the results of perfusion studies in the uterus, placenta, and fetuses to determine a relation between the dose of drug, hemodynamic changes, and degree of histopathologic findings. Forty-seven Wistar albino rats and 91 rat fetuses were studied: group A (pregnant rats), 16 rats and 91 rat fetuses, group B (nonpregnant rats), 31 rats. Each group was divided into subgroups of cocaine-abused and non-cocaine-abused rats. In each group 2-3 mCi technetium Tc-99m methoxyisobutyl-isonitryl (Sesta MIBI) was injected into the tail vein. Radioactivity counts per g tissue (cps/g) in the uterus, placenta, and fetus were assessed by gamma counter. Cocaine 75 mg/kg per day severely decreased the perfusion of the uterus, placenta, and fetuses. These impairments were statistically significant. In lower doses (30-50 mg/kg per day) no statistically significant changes were observed in the perfusion of the uterus and placenta, but a significant decrease in fetal perfusion was seen. In group B, no significant changes in the perfusion of the uterus due to cocaine were seen. Thus, maternal cocaine abuse results in a reduction in perfusion of the uterus, placenta, and fetus. There was a dose-dependent correlation between the perfusion changes and the development of NEC-like histopathologic changes: the higher the cocaine dose received by the mother, the higher the level of placental and fetal injury. We suggest that perinatal cocaine exposure should be considered a high risk for development of NEC in rat fetuses and embryos. For this reason, infants with a history of possible maternal cocaine abuse or positive urinary cocaine metabolites have to be followed very carefully for NEC.

Animals↗
PubMed2000

Multidisciplinary approach to Wilms' tumor: 18 years of experience.

BACKGROUND: The aim was to evaluate the characteristics of Wilms' tumor and the results of combined modality treatment obtained in our center in Turkey. METHODS: From January 1978 to December 1996, 106 patients with Wilms' tumor were diagnosed. Of these 106 patients, 61 were male and 45 were female (M/F = 1,35); the median age at diagnosis was 39 months. The distribution of the 106 patients according to clinical stage was stage I 10%, stage II 42%, stage III 35%, stage IV 9% and stage V 4%. Histologically, 102 of the cases could be evaluated: favorable histology was diagnosed in 88.2% and unfavorable histology in 11.8% of the patients. Ninety-eight patients were treated according to NWTS and eight patients according to SIOP protocols. RESULTS: The EFS and overall survival rates at 2 years were 74.2 and 79.5% respectively, and at 5 years 72.4 and 76.6% respectively. CONCLUSION: As a developing country we evaluated our survival rates and report an improvement in treatment in recent years.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1999

Lymphangiosarcoma in chronic lymphoedema. Stewart-Treves syndrome.

A case of post-mastectomy lymphangiosarcoma is reported. Lymphangiosarcoma is an extremely rare but highly lethal complication of chronic lymphoedema. Our patient was treated by amputation and died 6 months later. Treatment of post-mastectomy lymphangiosarcoma is still unsatisfactory. Early recognition and radical ablative surgery seem to provide best chance for survival.

Amputation, Surgical↗
PubMed1998

Congenital short bowel syndrome associated with appendiceal agenesis and functional intestinal obstruction.

Congenital short bowel with functional intestinal obstruction and absence of appendix vermiformis is a very rare condition with poor prognosis. Seventeen cases of congenital short bowel have been reported previously in the literature. The etiology is unknown. In this report, a case of congenital short bowel, combined with functional intestinal obstruction, mal-rotation, and absence of the appendix vermiformis, is presented and the pathogenesis discussed.

Appendix↗
PubMed1998

Comparison of different bladder autoaugmentation techniques in a rabbit model.

OBJECTIVE: To compare the urodynamic, radiological and histopathological findings of a bladder autoaugmentation method combined with different seromuscular enterocystoplasty (mucosectomized) techniques and the rectus abdominis muscle-flap (RAMF) technique, and thus devise a method that may eliminate the potential risks of bladder autoaugmentation. MATERIALS AND METHODS: The study comprised 20 male New Zealand White rabbits in four groups. In group 1 (control), an autoaugmentation model was created by incising the detrusor muscle of the bladder, forming a large wide-mouthed bladder diverticulum. In group 2, a full-thickness RAMF with an intact vascular supply was used to cover the augmented bladder wall. In groups 3 and 4, besides the autoaugmentation procedure, a 5 cm ileal segment with an intact vascular supply was prepared and the muscosal layer of the segment removed. In group 3, the mucosectomized surface of the ileal graft was used to cover the autoaugmented bladder (seromuscular ileocystoplasty, SMEC) and in group 4, the serosal surface of the pedunculated ileal graft was used in reverse to cover the autoaugmented bladder wall (reversed SMEC, RSMEC). All groups were followed using intravenous pyelography (IVP), voiding cysto-urethrography (VCUG), urodynamic investigations and histopathological analysis for 2 months post-operatively. RESULTS: In all groups except 2 (RAMF), a diffuse bulging of the bladder wall was detected on IVP and VCUG. In groups 1, 3 and 4, there was a significant increase in mean bladder capacity and the compliance also increased. However, in group 2 there was a significant reduction in bladder capacity and compliance, and the histopathological analysis showed severe fibrosis. The fibrotic changes were moderate in groups 1 and 4 and mild in group 3. CONCLUSIONS: The urodynamic studies, IVP, VCUG and histopathology suggested that the SMEC technique decreased the potential risks of bladder autoaugmentation, had minimal side-effects and was the most appropriate coat over the uroepithelium after bladder autoaugmentation.

Animals↗
PubMed1998

A modified Mitrofanoff procedure using the rectus abdominis muscle flap technique: a preliminary report in a rabbit model.

OBJECTIVE: To create a modified Mitrofanoff continent stoma in a rabbit model, using a rectus abdominis muscle flap (RAMF) with its intact vascular supply. MATERIALS AND METHODS: Fifteen New Zealand White male rabbits underwent an operation to create a 6 cm long tube using a RAMF with its vascular pedicle from the inferior epigastric vessels. The internal orifice of the tube was anastomosed to the anterior bladder wall with 5/0 polyglactin sutures. The external orifice was directly sutured to the skin to fashion a catheterizable Mitrofanoff stoma. A 6 F catheter was left in the tube as a stent for 2 weeks. Radiography and histopathological investigations were carried out for 8 weeks after surgery. RESULTS: Intravenous pyelography and voiding cystourethrography showed a normal looking urinary system; contrast studies showed an intact RAMF tube with no signs of stricture. Catheterization of the stoma was possible in each animal. Histopathologically, there was no evidence of severe fibrosis or inflammatory changes; the inner surface of the tube (proximal bladder side) was covered by transitional urinary epithelium in most of the animals. CONCLUSION: It is possible to create a Mitrofanoff tube using a vascularized RAMF in a rabbit model. Using this technique there is no need to perform a major operation in the gastrointestinal system. The technique may provide a good alternative treatment in those patients who need a combination of Mitrofanoff and antegrade continence enema surgery, and in patients with previous appendectomy.

Animals↗
PubMed1997

Urodynamic investigations in reversed ileal seromuscular enterocystoplasty: an experimental study in a rabbit model.

OBJECTIVE: To investigate the efficacy of the reversed ileal seromuscular enterocystoplasty (RISMEC) technique and assess the urodynamic findings. MATERIALS AND METHOD: A large bladder defect was created in 12 New Zealand White rabbits: in six the bladder was closed primarily (control group) and in the remaining six the bladder was augmented using the RISMEC technique combined with omentoplasty. The results were assessed using intravenous pyelography (IVP), voiding cysto-urethrography (VCUG), histopathological investigations and urodynamic studies after 4 and 12 weeks. RESULTS: The IVP and VCUG revealed an apparently normal urinary system and voiding pattern in both groups. Histopathologically, in all rabbits undergoing RISMEC, the serosal surface of the reversed ileum was lined with transitional urinary epithelium; there was no evidence of severe fibrosis, inflammation, stone or mucus-production. Urodynamic studies showed a significant decrease in the mean bladder capacity in the controls, but no significant change in the RISMEC group. The mean bladder compliance decreased from 5.85 to 1.36 mL/cmH2O in the controls (P < 0.02) but there was no significant decrease in compliance in the RISMEC group. CONCLUSION: The results suggest that in this rabbit model, the RISMEC technique combined with omentoplasty increases bladder capacity with minimal graft shrinkage; the peritoneal surface is rapidly covered with transitional epithelium and the integrity of the kidneys preserved. Urodynamic investigations showed neobladders with adequate storage capacity, low intravesical pressures and improved compliance rates.

Animals↗
PubMed1996

Rectus abdominis muscle flap (RAMF) technique for the management of large bladder defects: urodynamic findings in a rabbit model.

OBJECTIVE: To investigate the efficacy of the rectus abdominis muscle flap (RAMF) technique for the closure and augmentation of small, inelastic bladder extrophies using a rabbit model with a large bladder defect. MATERIALS AND METHODS: Fifteen New Zealand White rabbits were studied in two groups; nine rabbits in group 1 underwent resection of 65% of the bladder and then bladder augmentation using a full-thickness RAMF, including peritoneum, and six rabbits in group 2 (control) underwent a similar bladder resection but the bladder remnant was closed primarily. Urodynamic investigations were carried out in both groups preoperatively and 4 weeks after surgery. Intravenous pyelography (IVP), voiding cystourethrography (VCUG), urodynamic and histopathological investigations were also carried out for up to 4 months after surgery. RESULTS: As assessed by IVP, the urinary system appeared normal, with no signs of upper urinary tract dilatation and VCUG showed a normal voiding pattern. After surgery, the bladder capacity and compliance decreased in both groups, but both variables partially recovered in group 1, although not significantly. CONCLUSIONS: This animal model indicates that the RAMF technique is a suitable alternative for the management of large bladder defects and bladder exstrophies with small, inelastic, non-compliant bladders.

Animals↗
PubMed1995

Osteogenic potential of infant dural grafts in different recipient beds.

An experimental study in adult rats was designed to test whether infant dura, when transplanted as an isograft to different recipient beds, can maintain its osteogenic potential. There was bone regeneration in more than 50% of the defects in all animals in which infant dura was present. There was minimal bone regeneration in defects in which adult dura remained alone and in which the dural defect repaired with adult dural graft. Ectopic bone did not form on the abdominal fascia or in the abdominal muscles from either infant or adult dural transplantation.

Animals↗
PubMed1994

Cytologic changes induced by 131I in the thyroid glands of patients with hyperthyroidism; results of fine needle aspiration cytology.

Cytological changes in the thyroid glands of 120 patients who had received 131I treatment for hyperthyroidism were examined in detail. The cytological material was obtained by fine needle aspiration cytology. The patients were divided into four groups according to the amount of time elapsed after the 131I treatment. The control groups were selected from euthyroid patients with hypoactive or non-functional solitary nodules and patients with Graves' disease who had not received 131I therapy. The thyrocytes revealed degenerative changes in the immediate period after cessation of treatment. One year after the treatment some non-specific changes, such as oxyphilic metaplasia and regeneration, were seen. No malignant change has been observed clinically or cytologically during up to 20 years of follow up since receiving 131I.

Biopsy, Needle↗
PubMed1992

Silver binding nucleolar organizer regions in adrenocortical neoplasia.

The possible contribution of the silver colloid technique for staining nucleolar organizer regions in the distinction between benign and malignant adrenocortical neoplasms was investigated. Nine cases of adenoma, eight cases of carcinoma, nine cases of hyperplasia and four normal adrenal cortex specimens were examined. The mean silver binding nucleolar organizer region (Ag-NOR) value in adenoma was 4.29, and in carcinoma 7.16 (P less than 0.001). Adenomas with diameters greater than 3 cm had significantly higher Ag-NOR counts than smaller adenomas. For normal cortex, the mean Ag-NOR value was 2.05 and in hyperplasia, 3.62. The results indicate that the Ag-NOR technique can help in differential diagnosis between benign and malignant adrenocortical lesions and thus may have a prognostic value.

Adenoma↗