PubMed Health⌕ Search

Biomedical subjects

S Dienstman

Publications and source records attributed to S Dienstman.

3 recordsLinked to original sources
PubMed1975

Effects of cytochaslasin B and colcemide on myogenic cultures.

Muscle cultures treated with cytochalasin B yield mono- and oligonucleated cells of two kinds: (i) arborized, replicating precursor myogenic cells and fibroblasts; and (ii) round, post-mitotic, terminally differentiating myoblasts and myotubes. The arborized cells do not bind fluorescein-labeled antibody against myosin, do not contract rhythmically, and do not display hexagonally stacked thick and thin filaments. The round, mono-nucleated myoblasts and round, oligonucleated myotubes bind the fluorescein-labeled antibody against myosin, contract rhythmically, and display clusters of hexagonally-stacked thick and thin filaments. When cytochalasin B is removed and replaced by colcemide, the arborized cells, but not the post-mitotic muscle cells, acquire a radial symmetry and are induced to assemble massive, meandering cables that may occupy over 25% of the cell volume. These tortuous calbes are positively birefringent and consist exclusively of enormous numbers of 100-A, intermediate-sized filaments.

Animals↗
PubMed1974

The phenotypic complexity of myogenic clones.

A single cell isolated from cultured 8-day leg muscle may, when subcultured, yield a myogenic clone. A myogenic clone consists of myotubes and mononucleated cells. When such a myogenic clone is subcultured, large numbers of mononucleated cells are recovered. These mononucleated cells histologically and biochemically are indistinguishable from authentic fibroblasts cultured under the same conditions: they synthesize (alpha1)(2)alpha2 chains of collagen, large amounts of hyaluronic acid, and modest amounts of chondroitin sulfate. These mononucleated cells, however, will not chondrify when grown under culture conditions known to permit presumptive chondroblasts to differentiate terminally. These findings demonstrate that there is a population of single cells in 8-day muscle that is neither a myoblast nor a fibroblast, but is the common progenitor for cells in the myogenic and fibrogenic lineages: this progenitor, however, is beyond the point of readily yielding chondrogenic cells. These findings are discussed in terms of the limited number of phenotypic options open to differentiating cells in each of the successive compartments of their respective lineages.

Animals↗