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S Dottorini

Publications and source records attributed to S Dottorini.

At least 19 recordsLinked to original sources
PubMed1985

Echinococcus granulosus: diagnosis of hydatid disease in man.

Several antigen fractions were prepared from sheep hydatid fluid and scolices of Echinococcus granulosus by salting out with ammonium sulphate. Sera from subjects with hydatid disease and from uninfected controls were assayed by the IHA, Latex, ELISA and Complement Fixation tests. The greatest sensitivity was given by the hydatid fluid 0.8 M fraction in the IHA test. This antigen also gave good results with the ELISA technique. Antigens from sheep fertile fluid were diagnostically superior to those from scolices. The specificity was excellent for all antigens examined.

Antibodies↗
PubMed1984

Echinococcus granulosus: additional studies on selected sera from seronegative patients with hydatidosis.

Human hydatidosis-positive sera, which resulted negative in serological tests but positive on surgery, were examined with I.H.A. and E.L.I.S.A. techniques using a standardized antigen and a new antigen (the 0.8 M antigen) from sheep hydatid fluid. The present study demonstrated that the 0.8 M antigen was the most effective, and that sensitivity of any given antigen depends upon the method adopted.

Adolescent↗
PubMed1983

Hydatidosis: a first approach for serological standardization.

Hydatid fluids were collected in three continents and used as antigens against both human E. granulosus and E. multilocularis hydatidosis-positive sera collected in the same countries, in ELISA. The sensitivity of the antigens was good for all sera examined and this confirms the existence of common antigens, which is particularly marked in the 0.8M fraction of Italian hydatid fluid.

Antibodies↗
PubMed1976

Sialic acid content in four cell strains transformed by adenoviruses and/or SV40.

The sialic acid content in hamster cells primarily transformed by adenovirus was found lower than in cells transformed by papovavirus SV40. Intermediate concentrations were shown in a cell strain transformed by an adenovirus-SV40 hybrid. An adenovirus 19 - transformed cell strain derived from the NIL-2 hamster cell line, adapted to unlimited growth in vitro prior to the exposure to the adenovirus, revealed a high content of sialic acid. The concentration of sialic acid in cells transformed by DNA-viruses may depend both on the transforming virus and on the preceding history of the cell line. The tumors revealed more sialic acid than the in vitro cultures derived from the same cell strains. This finding is discussed.

Adenoviridae↗
PubMed1975

Characterization of the T antigen induced by SV40.

3 or 4 antigenic SV40 specific components were established in hamster tumors induced by SV40-transformed cells, using linear sucrose gradient centrifugation and Sephadex G-200 column chromatography. The lightest component, approximate molecular weight 21,000, did not inhibit the nuclear staining of SV40-transformed cells in the fluorescent antibody inhibition test.

Alcohol Oxidoreductases↗
PubMedDate not supplied

Lacalization of the SV40 T antigen in hamster cells transformed by PARA(3ct)-adenovirus 7.

The localization of the SV40 T antigen in hamster cells transformed by the PARA(3ct)-adenovirus 7 variant and, for comparison, by SV40 was examined. The H50 cell strain (transformed by SV40) and the P7-/BL/SV/OD strain (transformed by PARA(3ct)-adenovirus 7) showed a preferential localization of the SV40 T antigen in the nucleus both by immunofluorescence and by complement fixation. On the other hand, two different PARA(3ct)-adenovirus 7-transformed cell lines (P7-/Ar/d/15A and P7/Ar/m/14A) showed an almost equivalent concentration of the SV40 T antigen in the nucleus and in the cytoplasm both by immunofluorescence and complement fixation. The H50, P7-/BL/SV/3D, P7/Ar/m/14A cells were oncogenic for hamsters; the P7/Ar/d/15A line was not.

Adenoviridae↗