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Biomedical subjects

S Douglass

Publications and source records attributed to S Douglass.

11 recordsLinked to original sources
PubMed2001

Occurrence of Legionella in groundwater: an ecological study.

The natural habitat of Legionella is the water environment. Little is known about their presence in groundwater in spite of the fact that many millions around the globe regularly rely on groundwaters. This pilot study was aimed at evaluating the occurrence of Legionella in groundwater samples (water and biofilms) collected from various sites. Water and biofilm samples from selected groundwater sources were examined for Legionella using culture media (selective and non-selective) and a semi-nested PCR assay. Innovative approaches such as immunomagnetic separation (IMS) in combination with cultivation and flow cytometry were also evaluated. The findings available thus far show that (a) Legionella could be readily recovered from groundwater samples by cultivation even though their numbers showed considerable variations, (b) surprisingly, the PCR methodology was not yet as sensitive as cultivation and (c) flow cytometry was not directly applicable on natural samples because of debris and the high number of heterotrophic associated microflora from which some members were likely to cross-react with the monoclonal antibody used for separation procedures (IMS).

Biofilms↗
PubMed2001

Tower of Hanoi: evidence for the cost of goal retrieval.

Past research on the Tower of Hanoi problem has provided clear evidence for the importance of goal-subgoal structures in problem solving. However, the nature of the traditional Tower of Hanoi problem makes it impossible to determine whether there is any special cost associated with storing or retrieving goals. A variation of the Tower of Hanoi problem is described that allows one to determine separately if there is an effect of how long a goal has to be retained on storage time or how long ago it was formed on retrieval time. This paradigm provides evidence for an effect of retention interval on retrieval time and not on storage time. An ACT-R (Adaptive Control of Thought-Rational) simulation of these data is described, which treats goal memory as no different from other memories.

Adult↗
PubMed2000

Long-term blockade of the expression of cocaine sensitization by ondansetron, a 5-HT(3) receptor antagonist.

Intermittent cocaine administration induces sensitization (reverse tolerance) to its behavioral effects. The mechanism(s) mediating sensitization is not clear, however, previous research has implicated 5-HT(3) receptors in the expression of sensitization. The present experiment evaluated the ability of the 5-HT(3) receptor antagonist, ondansetron, administered during withdrawal from chronic intermittent cocaine administration, to block the expression of sensitization. Rats were pretreated for 14 days by daily subcutaneous injections of either 40 mg/kg cocaine or 0.9% saline. During the first 5 days of withdrawal from this pretreatment regimen, all rats received a daily subcutaneous injection of 0-1.0 mg/kg ondansetron. On days 7, 14 or 28 of withdrawal from the cocaine pretreatment, the rats received a 15.0-mg/kg cocaine challenge. Ambulatory behavior was automatically recorded for 60 min. Ondansetron had no significant effect on the subsequent behavioral response to cocaine in the saline control subjects. In contrast, daily injections of ondansetron blocked the expression of sensitization at all withdrawal times. We thus report that it is possible to permanently block the expression of sensitization once it has developed by administering a 5-HT(3) receptor antagonist.

Animals↗
PubMed1999

Practice and retention: a unifying analysis.

What is the strength of a memory trace that has received various practices at times tj in the past? The strength accumulation equation proposes the following: strength = sigma tj-d, where the summation is over the practices of the trace. This equation predicts both the power law of practice and the power law of retention. This article reports the fits of the predictions of this equation to 5 experiments. Across these experiments, participants received as many as 240 trials of practice distributed over intervals as long as 400 days. The experiments also varied whether participants were just practicing retrieving an item or practicing applying a relatively complex rule. A model based on this equation successfully fit all the data when it was assumed that the passage of psychological time slowed after the experimental session. The strength accumulation equation was compared with other conceptions of the retention function and the relationship of the retention function to the practice function.

Humans↗
PubMed1997

The role of examples and rules in the acquisition of a cognitive skill.

In 3 experiments, participants memorized 8 examples, each exemplifying a different rule. Participants were asked to extend these rules to new examples. They practiced applications of the rules to examples over a period of 4 days (Experiment 1) or 5 days (Experiments 2 and 3). Although these rules were bidirectional, an asymmetry gradually built up such that participants became more facile in using the rules in the practiced direction. Participants also showed an advantage when the initial study example was repeated or when test examples were repeated. It is argued that skill acquisition involves development of a complex set of strategies based on use of rules and retrieval of examples. Four overlapping stages of skill acquisition are described.

Analysis of Variance↗