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Biomedical subjects

S Du

Publications and source records attributed to S Du.

At least 19 recordsLinked to original sources
PubMed2007

Cross-sectional comparison of physical activity and inactivity patterns in Chinese and Filipino youth.

BACKGROUND: To compare and contrast youth physical activity (PA) and inactivity patterns in two developing Asian countries: the Philippines and China. METHODS: Comparative analysis of 1997-1999 Cebu Longitudinal Health and Nutrition Survey and the 1997 China Health and Nutrition Survey, large-scale surveys that included questions on type, frequency and duration of: commuting mode to school, sports/exercise in and outside of school, select sedentary activities and chores. RESULTS: Filipino data included 760 males and 872 females aged 14-16 years. The comparable Chinese sample consisted of 202 males and 197 females. Active commuting is proportionately high in both countries (70-71% in the Philippines vs. 77-90% in China), although commuting by bicycling is rare in the Philippines (<1%) vs. China ( approximately 35%). Patterns of school sport/exercise participation differ between countries by gender; more Filipino males report school sport/exercise than females (63 vs. 49%) vs. China, where more females participate than males (75 vs. 69%). Sport/exercise outside of school is proportionately low (6-12%) for youth from both countries with a single exception: 74% of Filipino males participate in extra-curricular sport/exercise. Although a higher percentage of Filipino youth report watching television >4 h/day (<10%) vs. Chinese youth (<1%), both are lower than comparable US reports. CONCLUSION: In the Philippines, continued modernization augurs a decrease in local primary PA sources (chores and active commuting). In China, where youth already are not expected to perform chores, shifts to more passive commuting modes (i.e. increased motorized transportation) are anticipated.

Activities of Daily Living↗
PubMed2006

Trends in the distribution of body mass index among Chinese adults, aged 20-45 years (1989-2000).

OBJECTIVE: To describe body mass index (BMI, in kg/m(2)) distribution patterns and trends among Chinese adults, aged 20-45 years (1989-2000). DESIGN: A descriptive, population-based study of BMI change. SETTING: Chinese provinces (eight in 1989 and 1997; nine in 2000), representative of the household-based surveys (the China Health and Nutrition Survey, 1989-2000) using multistage, random cluster sampling, supplemented with annual household consumption survey data of the State Statistical Bureau (SSB). SUBJECTS: A total of 4527, 4507 and 4046 adults, aged 20-45 years, in 1989, 1997 and 2000, respectively. MEASUREMENTS: BMI (underweight: BMI<18.5 kg/m(2) and overweight: BMI>/=25 kg/m(2)). Percentile curves for BMI in 1989 and 2000 were constructed by gender and age using the LMS (lambda, mu, sigma) method. RESULTS: Compared with 1989, the 2000 BMI distribution curves flattened at higher levels of BMI (men and women). There was a 13.7% increase in the proportion of men and a 7.9% increase of women who were overweight or obese with a resulting greater change in the annualized prevalence rate for men. This increase in the prevalence of overweight and obesity was far greater than the decrease (2.1% for men; 2.2% for women) in that of underweight. Age-gender-specific percentile curves showed BMI increases mainly among women, aged 35-45 years, and among men at all age groups. CONCLUSIONS: Chinese BMI dynamics show much greater rates of change among men, aged 20-45 years, than among women, with the increase among women concentrated between ages 35 and 45 years. These changes portend large shifts in other diet-related non-communicable diseases in China over the following decades. Controlling the increasing trends of BMI, especially in men, is an important public health problem facing China.

Adult↗
PubMed2006

Studies on the encapsulation of oxymatrine into liposomes by ethanol injection and pH gradient method.

Oxymatrine is the major active alkaloid constituent extracted from traditional Chinese herb medicine Sophora flavescens Ait (Kushen) and Sophora alopecuroides (Kudouzi). In recent years, oxymatrine had been found to posses remarkable anti-hepatic activity and has been used for treating hepatitis B in clinical therapy in China. In this study, we attempted to entrap oxymatrine into liposomes to facilitate the delivery of oxymatrine to the liver and enhance the therapeutic efficiency for hepatitis. Preformulation investigation was performed to obtain the drug physicochemical properties such as solubility, pKa, and logP for rational liposomes preparation design. Liposomes were prepared with soybean lecithin by ethanol injection and pH gradient loading methods. At the same time the factors affecting the entrapment efficiencies were investigated and compared. Ethanol injection method yielded liposomes with entrapment efficiency less than 20%. The lipid composition and aqueous medium had some effects on entrapment efficiency. However, liposomes could be produced with entrapment efficiency above 50% by pH gradient method. The internal pH buffer capacity, the lipid composition, and drug-to-lipid ratio greatly influenced the entrapment efficiency, while the incubation temperature had almost no effect on entrapment efficiency in the active loading procedure.

1-Octanol↗
PubMed2006

Measuring the full economic costs of diet, physical activity and obesity-related chronic diseases.

Most studies that have focused on the costs of obesity have ignored the direct effects of obesity-related patterns of diet and physical activity. This study reviews the full effects of each component--poor dietary and physical activity patterns and obesity--on morbidity, mortality and productivity. The direct healthcare costs are based on a review of the effects of these factors on key diseases and the related medical care costs of each disease. The indirect costs on reduced disability, mortality and sickness during the period of active labour force participation prior to retirement are also examined. A case study is prepared for China to provide some guidance in the utilization of this review for economic analysis of obesity. The case study shows that the indirect costs are often far more important than the direct medical care costs. The Chinese case study found that the indirect effects of obesity and obesity-related dietary and physical activity patterns range between 3.58% and 8.73% of gross national product (GNP) in 2000 and 2025 respectively.

Absenteeism↗
PubMed2003

Physical activity and inactivity in Chinese school-aged youth: the China Health and Nutrition Survey.

OBJECTIVE: To describe physical activity (PA) and inactivity levels and patterns in Chinese school children (aged 6-18 y). DESIGN: PA and inactivity were assessed in a youth cohort enrolled in the China Health and Nutrition Survey (CHNS) in 1997. SUBJECTS: A total of 1423 males (11.5+/-3.2 y) and 1252 females (11.5+/-3.3 y). MEASUREMENTS: PA and inactivity were assessed by self-reported usual activity (questionnaire). Children under 10 y were assisted by parents. RESULTS: Approximately 84% of Chinese youth actively commute to school for a median of 100-150 min/week. A total of 72% engage in in-school moderate/vigorous (MOD/VIG) PA for a median of 90-110 min/week. Relatively few children ( approximately 8%) participate in any MOD/VIG PA outside of school. A total of 72% engage in study-related activities outside of school for a median of 420 min/week. Only 8% of Chinese school children, regardless of gender, watch television > or =2 h/day; less than 1% watch > or =4 h/day. Chores related to housework are not a part of life for Chinese school children; fewer than 20% performed these tasks. CONCLUSION: Chinese youth are unique compared to those in other developing countries because they do not perform household chores. Instead, they are under pressure to achieve scholastically. Participation in MOD/VIG PA outside of school is almost nonexistent. Current television watching habits are relatively low compared to developed countries and walking/biking is a common form of commuting. The descriptive analysis herein represents the foundation upon which future longitudinal studies of PA in this population will be based.

Adolescent↗
PubMed2001

Synthesis and reactivity of the monocarbon molybdenacarborane anion [1,2-mu-NHBu(t)-2,2,2-(CO)(3)-closo-2,1-MoCB(10)H(10)](-).

The reagent Li[7-NHBu(t)()-nido-7-CB(10)H(12)] reacts with [Mo(CO)(6)] in NCMe at reflux temperatures, followed by addition of [N(PPh(3))(2)]Cl, to give [N(PPh(3))(2)][1,2-mu-NHBu(t)-2,2,2-(CO)(3)-closo-2,1-MoCB(10)H(10)] (1). The tungsten (2) and chromium (3) analogues were similarly obtained, but the latter is unstable and was isolated in low yield. An X-ray diffraction study of 2 confirmed that the exo-polyhedral NHBu(t) group forms a bridge between the cage-carbon atom and the tungsten. For 1, this intramolecular donor bond is lifted on protonation in the presence of donor molecules L (CO, PPh(3), PMe(3), PEt(3), PMe(2)Ph) when zwitterionic complexes [1-NH(2)Bu(t)()-2,2,2-(CO)(3)-2-L-closo-2,1-MoCB(10)H(10)] (4) are formed. In contrast, protonation with HCl gives a salt [N(PPh(3))(2)][1-NH(2)Bu(t)()-2,2,2-(CO)(3)-2-Cl-closo-2,1-MoCB(10)H(10)] (5). Complex 1 in CH(2)Cl(2) with CNBu(t) is oxidized by iodine, affording [1,2-mu-NHBu(t)()-2,2,2-(CNBu(t)())(3)-2-I-closo-2,1-MoCB(10)H(10)] (6a). Treatment of 1 with [CuCl(PPh(3))](4) in the presence of Tl[PF(6)] yields the bimetallic compound [exo-[Cu(PPh(3))]-1,2-mu-NHBu(t)-2,2,2-(CO)(3)-closo-2,1-MoCB(10)H(10)] (8), whereas reaction with [AuCl(PPh(3))] and Tl[PF(6)] affords a mixture of [1,2-mu-NHBu(t)-2-[Au(PPh(3))]-2,2,2-(CO)(3)-closo-2,1-MoCB(10)H(10)] (9) and [Au(PPh(3))(2)][2,2'-mu-Au-[1,2-mu-NHBu(t)-2,2,2-(CO)(3)-closo-2,1-MoCB(1)(0)H(10)](2)] (10a). In solution, 9 disproportionates, giving 10a. The [N(PPh(3))(2)](+) salt (10b) is readily prepared by treating 1 with [AuCl(THT)] (THT = tetrahydrothiophene) and Tl[PF(6)], and its structure was determined by X-ray diffraction.

Journal Article↗
PubMed2001

The neuroprotective agent ebselen modifies NMDA receptor function via the redox modulatory site.

Ebselen is a seleno-organic compound currently in clinical trials for the treatment of ischemic stroke and subarachnoid hemorrhage. Its putative mode of action as a neuroprotectant is via cyclical reduction and oxidation reactions, in a manner akin to glutathione peroxidase. For this reason, we have investigated the effects of ebselen on the redox-sensitive NMDA receptor. We have found that ebselen readily reversed dithiothreitol (DTT) potentiation of NMDA-mediated currents in cultured neurons and in Chinese hamster ovary (CHO) cells expressing wild-type NMDA NR1/NR2B receptors. In contrast, ebselen was unable to modulate NMDA-induced currents in neurons previously exposed to the thiol oxidant 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), or in CHO cells expressing a mutant receptor lacking the NR1 redox modulatory site, suggesting that ebselen oxidizes the NMDA receptor via this site. In addition, ebselen was substantially less effective in modifying NMDA responses in neurons exposed to alkylating agent N-ethylmaleimide (NEM) following DTT treatment. Ebselen also reversed DTT block of carbachol-mediated currents in Cos-7 cells expressing the alpha(2)beta delta epsilon subunits of the acetylcholine receptor, an additional redox-sensitive ion channel. Ebselen was observed to significantly increase cell viability following a 30-min NMDA exposure in cultured neurons. In contrast, other more typical antioxidant compounds did not afford neuroprotection in a similar paradigm. We conclude that ebselen may be neuroprotective in part due to its actions as a modulator of the NMDA receptor redox modulatory site.

Animals↗
PubMed2001

Vaccinia virus telomeres: interaction with the viral I1, I6, and K4 proteins.

The 192-kb linear DNA genome of vaccinia virus has covalently closed hairpin termini that are extremely AT rich and contain 12 extrahelical bases. Vaccinia virus telomeres have previously been implicated in the initiation of viral genome replication; therefore, we sought to determine whether the telomeres form specific protein-DNA complexes. Using an electrophoretic mobility shift assay, we found that extracts prepared from virions and from the cytoplasm of infected cells contain telomere binding activity. Four shifted complexes were detected using hairpin probes representing the viral termini, two of which represent an interaction with the "flip" isoform and two with the "flop" isoform. All of the specificity for protein binding lies within the terminal 65-bp hairpin sequence. Viral hairpins lacking extrahelical bases cannot form the shifted complexes, suggesting that DNA structure is crucial for complex formation. Using an affinity purification protocol, we purified the proteins responsible for hairpin-protein complex formation. The vaccinia virus I1 protein was identified as being necessary and sufficient for the formation of the upper doublet of shifted complexes, and the vaccinia virus I6 protein was shown to form the lower doublet of shifted complexes. Competition and challenge experiments confirmed that the previously uncharacterized I6 protein binds tightly and with great specificity to the hairpin form of the viral telomeric sequence. Incubation of viral hairpins with extracts from infected cells also generates a smaller DNA fragment that is likely to reflect specific nicking at the apex of the hairpin; we show that the vaccinia virus K4 protein is necessary and sufficient for this reaction. We hypothesize that these telomere binding proteins may play a role in the initiation of vaccinia virus genome replication and/or genome encapsidation.

Amino Acid Sequence↗
PubMed2001

[Transition of dietary pattern in China].

The implications of dietary transition in China during the past 50 years were investigated. Data were collected from the representative national surveys, China Health and Nutrition Survey (1989-1997), China National Nutrition Survey (1982 and 1992), the Annual Household Consumption Surveys of the State Statistic Bureau and the Annual Death Report of China. In the early part of the major economic transformation, cereal intake increased before 1985 and decreased thereafter. There was also a long-term reduction of vegetable consumption, which has stabilized now. Intakes of animal foods increased slowly before 1979 and more quickly after the economic reforms occurred. The total energy intake has reduced, as energy expenditure has, large changes in the composition of energy have occurred. The proportion of energy from fat increased quickly and reached 27.3% in average and 32.8% in urban residents in 1997. More than one third of all Chinese adults and 58.4% of adults in urban areas consuming over 30% of their energy from fat. These changes resulted in rapid increases the prevalence of overweight and obesity and dietary-related chronic non-communicable diseases in urban residents. The traditional Chinese diet is shifting towards a diet with high fat, high energy density and low dietary fiber. The Chinese diets have entered into a new stage of transition.

China↗
PubMed2001

[Distributions of body mass index of Chinese adults].

Based on the Third China National Nutrition Survey conducted in 1992, the distribution of body mass index (BMI) in 44,485 adults aged 20-60 y was analyzed, and the cutoff points of BMI for overweight and obesity were also explored. The results showed that the BMI of Chinese adults was in logarithmic normal distribution. The average BMI of this population was 22.1 +/- 3.1. The 50th, 85th and 95th percentiles of BMI were 21.6, 24.9 and 27.4 kg/m2 respectively. The 85th percentile of BMI was much closer to the BMI cutoff points for overweight recommended by WHO, but the 95th percentile was much lower than WHO's cutoff points for obesity. The distribution of BMI based on age, gender and urban/rural areas was different and with statistical significance. It is considered that age and gender should be taken into account while setting up BMI cutoff points for the overweight and obesity of a population, as well as considering the relationships among BMI and morbidity and mortality, body fat composition, and some blood biochemical indexes.

Adult↗
PubMed2000

Structural and mechanistic investigation of 3-deoxy-D-manno-octulosonate-8-phosphate synthase by solid-state REDOR NMR.

15N¿(31)P¿ REDOR NMR experiments were applied to lyophilized binary complexes of 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8PS), with each of its natural substrates, phosphoenolpyruvate (PEP) and arabinose-5-phsophate (A5P), and with a mechanism-based inhibitor (K(i) = 0.4 microM), directly characterizing the active site basic residues involved in the binding of their carboxylate and phosphate moieties. KDO8PS was labeled uniformly with (15)N or [eta-(15)N(2)]Arg, and the ligands were selectively labeled with (13)C and (15)N. The NMR data established that PEP is bound by KDO8PS via a preserved set of structurally rigid and chemically unique Arg and Lys residues, with 5 A (upper limit) between epsilon-(15)N of this Lys and (31)P of PEP. A5P is bound in its cyclic forms to KDO8PS via a different set of Lys and Arg residues. The two sets arise from adjacent subsites that are capable of independent and sufficiently strong binding. The inhibitor is best characterized as an A5P-based substrate analogue inhibitor of KDO8PS. Five mutants in which highly conserved arginines were replaced with alanines were prepared and kinetically characterized. Our solid-state NMR observations complement the crystallographic structure of KDO8PS, and in combination with the mutagenesis results enable tentative assignment of the NMR-identified active site residues. Lys-138 and Arg-168 located at the most recessed part of the active site cavity are the chemically distinct and structurally rigid residues that bind PEP phosphate; R168A resulted in 0.1% of wild-type activity. Arg-63, exposed at the opening of the active site barrel, is the flexible residue with a generic chemical shift that binds A5P; R63A resulted in complete deactivation. The mechanistic implications of our results are discussed.

Aldehyde-Lyases↗
PubMed2000

NMDA and glutamate evoke excitotoxicity at distinct cellular locations in rat cortical neurons in vitro.

The development of cortical neurons in vivo and in vitro is accompanied by alterations in NMDA receptor subunit expression and concomitant modifications in the pharmacological profile of NMDA-activated ionic currents. For example, we observed that with decreasing NR2B/NR2A subunit expression ratio, the block of NMDA receptor-mediated whole-cell responses by the NR2B-selective antagonist haloperidol was also decreased. In mature cultures (>22 d in vitro), however, NMDA responses obtained from excised nucleated macropatches, which comprised a large portion of the soma, remained strongly antagonized by haloperidol. These results suggest that in more mature neurons NR1/NR2B receptors appear to be preferentially expressed in the cell body. As predicted from the whole-cell recording pharmacological profile, NMDA-induced toxicity was largely unaffected by haloperidol in mature cultures. However, haloperidol effectively blocked glutamate toxicity in the same cultures, suggesting that the neurotoxic actions of this amino acid were mostly due to the activation of somatic NMDA receptors. In experiments in which the potency of glutamate toxicity was increased by the transport inhibitor l-trans-pyrrolidine-2,4-dicarboxylic acid, the neuroprotective effects of haloperidol were significantly diminished. This was likely because of the fact that glutamate, now toxic at much lower concentrations, was able to reach and activate dendritic receptors under these conditions. These results strongly argue that exogenous glutamate and NMDA normally induce excitotoxicity at distinct cellular locations in mature mixed neuronal cultures and that NR1/NR2B receptors remain an important component in the expression of glutamate, but not NMDA-induced excitotoxicity.

ATP-Binding Cassette Transporters↗
PubMed2000

Rapid increase in immunoreactivity to GFAP in astrocytes in vitro induced by acidic pH is mediated by calcium influx and calpain I.

In higher vertebrates, reactive gliosis resulting from injury to the central nervous system (CNS) is characterized by a rapid increase in immunoreactivity (IR) to glial fibrillary acidic protein (GFAP). Little is known about the extracellular signals that initiate the increase in GFAP-IR following CNS injury. We demonstrated recently [T.H. Oh, G.J. Markelonis, J.R. Von Visger, B. Baik, M.T. Shipley, Acidic pH rapidly increases immunoreactivity of glial fibrillary acidic protein in cultured astrocytes, Glia 13 (1995) 319-322] that a rapid increase in GFAP-IR can be evoked in mature astrocyte cultures by exposing the cells to an acidic medium. We investigated the intracellular pathway(s) involved in initiating increased GFAP-IR, a hallmark of reactive astrocytes. The increase in GFAP-IR produced by exposure to acidic medium was blocked by pretreatment with nickel ions, by such blockers of L-type calcium channels as nifedipine, verapamil and diltiazem, by calpain inhibitor I, or by the intracellular calcium chelator, BAPTA-AM. At physiological pH, treatment with the calcium ionophore, A23187, resulted in increased GFAP-IR which could be blocked by pretreatment with calpain inhibitor I. Astrocytes exposed to low pH exhibited a marked increase in a GFAP fragment with a molecular weight of 48 kDa. In astrocytes exposed to acidic medium, alpha-fodrin, a selective endogenous substrate of calpain, was also found to be hydrolyzed producing fragments with molecular weights of 120-150 kDa. As anticipated, pretreatment with calpain inhibitor I prevented the proteolytic degradation of both GFAP and alpha-fodrin in these samples. These results suggest that the initial increase in GFAP-IR after CNS injury appears to be linked to Ca(++) influx, and is mediated further by a proteolytic process that seemingly involves the activation of the calcium-dependent protease, calpain I.

Acids↗
PubMed2000

Immunogenicity of porcine transmissible gastroenteritis virus spike protein expressed in plants.

Transgenic plants expressing recombinant proteins from pathogenic microorganisms provide an inexpensive edible vaccine for induction of local immunity. Three transgenic plant lines were generated expressing the spike (S) protein of transmissible gastroenteritis virus (TGEV), a protein crucial for establishing mucosal immunity. All three of them were driven by a strong plant promoter. One construct contained the 3.7 kb 5' end of the native S gene sequence. In the second construct part of the S gene, from nucleotide 49 to 1785, was modified for optimal plant recognition and was fused to a plant signal peptide coding sequence. The third construct contained the D epitope-coding region of the S gene, from nucleotide 1201 to 1591, which was fused to the alfalfa beta-amylase gene. The S gene products were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting. Antigens from all three transgenic plant lines induced TGEV-specific immune responses in pigs as determined by virus neutralization and ELISA, and the resultant antibody titers for all three constructs were similar.

Animals↗
PubMed2000

Role of tumor necrosis factor-alpha in neuronal and glial apoptosis after spinal cord injury.

We investigated the role of tumor necrosis factor (TNF)-alpha in the onset of neuronal and glial apoptosis after traumatic spinal cord crush injury in rats. A few TUNEL-positive cells were first observed within and surrounding the lesion area 4 h after injury, with the largest number observed 24-48 h after injury. Double-labeling of cells using cell type-specific markers revealed that TUNEL-positive cells were either neurons or oligodendrocytes. One hour after injury, an intense immunoreactivity to TNF-alpha was observed in neurons and glial cells in the lesion area, but also seen in cells several mm from the lesion site rostrally and caudally. The level of nitric oxide (NO) also significantly increased in the spinal cord 4 h after injury. The injection of a neutralizing antibody against TNF-alpha into the lesion site several min after injury significantly reduced both the level of NO observed 4 h thereafter as well as the number of apoptotic cells observed 24 h after spinal cord trauma. An inhibitor of nitric oxide synthase (NOS), N(G)-monomethyl-l-arginine acetate (l-NMMA), also reduced the number of apoptotic cells. This reduction of apoptotic cells was associated with a decrease in DNA laddering on agarose gel electrophoresis. These results suggest that: (i) TNF-alpha may function as an external signal initiating apoptosis in neurons and oligodendrocytes after spinal cord injury; and (ii) TNF-alpha-initiated apoptosis may be mediated in part by NO as produced by a NOS expressed in response to TNF-alpha.

Animals↗
PubMed2000

Preliminary studies on the application of retinal thickness analyzer in the diagnosis of glaucoma.

The patients with glaucoma underwent the examination of retinal thickness analyzer (RTA) to explore the diagnostic value of RTA in glaucoma. The retina of 6 mm x 6 mm size (approximately 20 degrees, centered on the macula) at the posterior pole was scanned by using RTA to obtain the images in 35 eyes of 22 patients with glaucoma. The images were processed by using SAS software package. The retinal thickness in the patients with glaucoma showed diffuse or local thinning. Twenty-seven eyes was definitely diagnosed as having glaucoma. There was a very significant difference in retinal thickness measurements by RTA between normal group and glaucomatous group (P = 0.0012). Except the measurements at the detected point 6 having no difference, the measurements at the detected point 3 showed a significant difference and the remaining 7 detected points presented a very significant difference between the two groups. Of the detected 9 points, the changes at the points 4, 8, and 9 were the most obvious. The discrete analysis was performed on the glaucomatous patients by a discriminant function established through the data at the detected points 4, 8 and 9 and the accurate estimate rate for the diagnosis of glaucoma was up to 80.77%. The measurements of RTA examination was consistent with the results of the vision field test. It was suggested that diffuse or local thinning of retinal thickness exists in the patients with glaucoma. The temporal inferior arcuate fibers and the papillomacular bundle between the macular and optic nerve heads showed a serious damage. The sensitivity of RTA examination was higher than visual field test.

Adolescent↗
PubMed2000

Current methods for estimating dietary iron bioavailability do not work in China.

Three current equations for estimating iron bioavailability were evaluated, and adjustments were proposed that would allow us to most effectively study iron bioavailability in China. Dietary intake data were obtained from 24-h dietary recalls taken over three consecutive days as part of the third Chinese National Nutrition Survey. Hemoglobin status was measured for 42,606 Chinese adults aged 18-60 y. The mean iron intake was 24.4 mg per capita per day, which was 177% of the Chinese RDA (209% of U.S. RDA). About 18% of the sample was classified as being anemic, indicating a large iron deficiency anemia and iron bioavailability problem in China. A number of methods proposed by World Health Organization and U.S. scholars were examined for adjusting iron bioavailability. Even the methods that consider several iron enhancers and inhibitors did not work adequately for the Chinese diet. The statistical assessment of the fit between iron bioavailability and hemoglobin status provided direction for adjusting the best of these predictive equations. We propose a new predictive approach for iron bioavailability which is more predictive of Chinese iron status. Consideration of additional dietary elements such as rice and bean consumption patterns are important. Our findings provide insight into additional factors which may influence iron bioavailability as well as possible improved methods for estimating the combined effect of multiple dietary factors on iron bioavailability, particularly in a vegetarian diet.

Adult↗