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Biomedical subjects

S E Huether

Publications and source records attributed to S E Huether.

12 recordsLinked to original sources
PubMed1985

The development of a rat/human skin flap served by a defined and accessible vasculature on a congenitally athymic (nude) rat.

Experience in microvascular surgery on rats and availability of athymic (nude) rats led us to believe that a long-term functional rat/human skin sandwich flap could be generated on a defined and experimentally accessible vasculature on nude rats. Such a system has been developed and validated. Microvasculature has been assessed. The volume of blood to the flap ranges from 1 to 2 ml/min, collateral circulation to the flap exists, but is negligible, and there is little change in the capillary blood flow as the flap ages. The flap can be utilized to study absorption of compounds from a half-cell diffusion chamber or from direct deposition on the skin, and can be utilized to study various parameters of percutaneous absorption, e.g., the effect of hydration on the stratum corneum. Transdermal flux can be determined. Altering the microcirculation directly affects the percutaneous absorption of compounds that are rapidly absorbed. The absorption of benzoic acid through an experimentally vasoconstricted area (iontophoresis of phenylephrine) significantly alters the time to peak absorption, with values being 14 times that of the control site. The system has been utilized to assess metabolic activity of skin in situ using [3H]adenine arabinoside and studying the appearance of its major metabolite, [3H]Ara-H, in flap blood, as well as the back diffusion of this compound into the donor chamber. Recently the human/rat skin sandwich flap component has been developed. With this system, it has been demonstrated that benzoic acid, when applied to the human skin component of the flap has an absorption profile which is quite different from that when benzoic acid is applied to rat skin, peak flux occurred 2 hr after application. This contrasts with 10 min to peak flux when the same experiment is carried out on the rat/rat skin sandwich flap. To our knowledge, the human/rat skin sandwich flap is the first example of a viable, functional human organ that is chronically maintained by a biologic support system which has the added distinction of being on an independent but accessible vasculature. The validation experiments strongly suggest that this system will be important in gaining insights into the more sophisticated in vivo components of skin, relative to toxicology and pharmacology.

Animals↗
PubMed1984

Patient's evaluation of argon laser therapy of port wine stain, decorative tattoo, and essential telangiectasia.

One hundred fourteen patients were studied to assess their perception of the results of argon laser therapy for port wine stains (PWS), tattoos, or essential telangiectasia of legs. At least 1 yr following treatment patients were surveyed using a 30-item mail questionnaire. A 91% response rate was achieved. The findings indicate that patients with PWS and tattoo treated with argon laser are moderately satisfied with the results and 85% of them would have treatment again. Laser therapy of these lesions should be continued. Treatment results of essential telangiectasia of the legs are disappointing and only 49% of patients would have treatment again. Discontinuance of argon laser therapy using the described techniques is recommended.

Adolescent↗
PubMed1983

How lasers work.

The thermal energy of the laser light beam allows simultaneous cutting, coagulation of vessels, and sterilization. The beam is produced by inducing a "population inversion" of excited atoms in a lasing medium. This stimulated emission of light is then amplified to create the lasing effect. Each type of laser causes a different tissue response consistent with its wavelength, energy fluence, and tissue absorption properties.

Argon↗
PubMedDate not supplied

Determination of normal variation in skin blood flow velocity in healthy adults.

This study noninvasively measured cutaneous microcirculatory blood flow velocity at six clinically significant sites on the feet, legs, and abdomen. Subjects were 51 healthy adult volunteers, 44 females and 7 males, with a mean age of 32 years. A laser Doppler velocimeter (LDV) was used to measure skin blood flow velocity. Skin temperatures and LDV measures were concurrently obtained from the dorsal surface of the feet, medial aspects of the lower legs, and at two contralateral midabdominal sites. Ambient and oral temperatures were repeatedly measured and remained stable among subjects. Using paired t tests, there were no statistical significances for contralateral comparisons of mean flow velocity and skin temperature differences. Regional differences among feet, legs, and abdomen were significantly different. The findings suggest that blood flow velocity of contralateral sites is similar. Unilateral circulatory disturbances such as surgical and nonsurgical traumas, primary cutaneous lesions, and venous or arterial occlusions are often encountered clinically. Nursing care measures to improve blood flow can be evaluated on the basis of contralateral flow rates.

Abdomen↗
PubMedDate not supplied

The microcirculation.

The geometric organization and control of the microbed varies with different organs and tissue elements. The moment-to-moment regulation of local blood flow occurs without dominant control by the extrinsic nervous system. This enhances the exchange of fluids, solutes and blood gases, and allows rapid redistribution of blood flow during states of stress and high metabolic demand.

Arterioles↗