Biomedical subjects

S E Tukachinskiĭ

Publications and source records attributed to S E Tukachinskiĭ.

14 recordsLinked to original sources

PubMed1977

[Effect of partial modification of serum albumin on its conformation and complex-forming activity].

In using a series of the succinic anhydride concentration the authors obtained preparations of succinylated albumin with a different degree of modification. The effect of succinylation on the immunochemical and some physico-chemical properties of albumin were investigated; depending on the concentration of the modifying reagent preparations of succinylated albumin proved to differ by the antigenic properties, electrophoretic mobility in agar gel and the complex-forming activity in interaction with rivanol. Conditions of partial modification permitting to increase the complex-forming properties of albumin 3-fold with the minimal changes in its structure were determined.

Chemical Phenomena↗

PubMed1976

[Change in specific antigen binding by rabbit lymphocytes during immunization with human serum albumin].

Dynamics of specific binding of the antigen with rabbit blood lymphocytes in the process of immunization was studied by radioimmunochemical method with the use of human serum albumin, I125-labeled. Three types of response to the antigenic stimulant were revealed: biphasic with two maximal of the antigen-binding activity -- on the 5th--10th and the 15th--20th day; uniphasic -- with the maximum activity on the 15th--20th day, the absence of increase of the antigen-binding activity of blood lymphocytes and a high mean level of specific antigens; the absence of primary cell reaction to the administration of the antigen and low level of specific antibodies. A correlation was revealed between the intensity of the humoral response and the sum total amount of the immunocompetent lymphocytes entering the circulation in the course of the whole immunization period.

Animals↗

PubMed1974