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Biomedical subjects

S Enomoto

Publications and source records attributed to S Enomoto.

At least 163 records · Page 9Linked to original sources
PubMed1989

Activation of oncogenes in human oral cancer cells: a novel codon 13 mutation of c-H-ras-1 and concurrent amplifications of c-erbB-1 and c-myc.

By NIH3T3 transfection assay in conjunction with in vitro transient neomycin selection, activated c-H-ras-1 oncogenes were detected in two squamous cell carcinoma cell lines, ZA and HOC-313, newly established from human oral cancer patients. ZA had a point mutational activation at the 13th codon, this activation of c-H-ras-1 being novel in human cancer cells, while HOC-313 appeared to have an activation at the 12th codon. In ZA, 16- to 32-fold amplification of the EGF receptor gene, c-erbB-1 and a few-fold amplification of c-myc were detected. The significance of these findings is discussed in relation to multistep carcinogenesis in human cells.

Aged↗
PubMed1989

[A case report of aortic and mitral regurgitation combined with ankylosing spondylosis].

The purpose of this communication is to present a successful case of surgical treatment of aortic and mitral valve regurgitation associated with ankylosing spondylosis. A 48-year-old male was admitted with symptoms of palpitation and shortness of breath. His heart murmur had been pointed out for 4 months prior to this admission. His previous history did not show any evidence of rheumatic heart disease. Retrograde aortography showed moderate to severe aortic valvular regurgitation and echocardiography revealed mitral valve regurgitation with minor degree. At the same time he complained of rigidity of neck muscle as well as back pain for last 15 years. He underwent aortic valve replacement and mitral valve was left alone. His postoperative course was uneventful. In Japan, aortic valve regurgitation associated with ankylosing spondylosis has been reported to be very rare compared to the European or American people. Several important features in the diagnosis and operative treatment for this combination of diseases have been also discussed.

Aortic Valve Insufficiency↗
PubMed1989

[Coronary artery dissection secondary to coronary arteriography: presentation of two cases and review of the literature].

Two reports of coronary artery dissection secondary to coronary arteriography and followed by emergency aorto-coronary bypass are presented. One case includes pathological specimen demonstrating medial and intimal change in the dissected coronary artery, which may have predisposed this artery to dissect. Also the literature on catheter-induced coronary artery dissection is reviewed.

Angiography↗
PubMed1989

[Mycotic abdominal aneurysm requiring emergency operation due to sudden thrombotic obstruction: a case report].

A 59-year-old man was admitted with history of low grade fever, general malaise and low back pain. Physical examination showed a pulsatile mass in the mid-abdomen. There were slight leucocytosis, elevated BSR and CRP. Abdominal ultrasonography, CT and MRI revealed an abdominal aneurysm within which there was an intimal flap and thrombus. Though blood culture was not positive, antibiotic therapy was initiated on the presumption of a mycotic abdominal aneurysm. Six days after admission, he complained of numbness in the lower extremities and both femoral pulsations were not palpable. Emergency surgery was undertaken. The aneurysm was adhered to the IVC and the ileum and there was a large thrombotic mass in it. Aneurysmectomy and Y-graft interposition were done uneventfully. Pathology revealed marked thickness of the aorta and infiltration of lots of inflammatory cells. The culture of aneurysmal wall was negative. This is the very rare case because of sudden thrombotic occlusion of the mycotic abdominal aneurysm. Several important features of this disease have been also discussed.

Aneurysm, Infected↗
PubMed1988

Biosynthesis of the epidermal growth factor receptor in human squamous cell carcinoma lines: secretion of the truncated receptor is not common to epidermal growth factor receptor-hyperproducing cells.

The biosynthesis of the EGF receptor was examined in the epidermoid carcinoma cell line A431 and five novel cell lines from human squamous cell carcinomas possessing high numbers of EGF receptors. Newly synthesized EGF receptors were visualized by labeling with [35S]methionine and immunoprecipitation with a monoclonal anti-EGF receptor antibody. In addition, the processing of the EGF receptor and its intracellular transport was analyzed by distinguishing cell surface receptors from intracellular receptors and by treating cells with inhibitors such as tunicamycin, monensin and brefeldin A. These analyses revealed that in all the tumor cell lines the EGF receptor is synthesized as a glycosylated protein of Mr 160,000 which is converted to the receptor of Mr 170,000 through posttranslational glycosylation. The receptors of Mr 160,000 and 170,000 appeared to possess high mannose type oligosaccharide chains because endoglycosidase H treatment reduced their molecular weights by approximately 30,000. A431 was the only tumor cell line studied that secreted the truncated EGF receptor of Mr 110,000. In A431 cells, the truncated EGF receptor was generated from a protein of Mr 60,000 through tunicamycin- and monensin-sensitive glycosylation. A431 cells treated with monensin secreted the truncated receptor as a Mr 95,000 form.

Acetylglucosaminidase↗
PubMed1988

[Extension of measurable range of film dosimetry by a low energy X-ray absorption method].

The availability of an X-ray absorption technique employing a very low power X-ray tube was examined to extend measurable range for photographic film dosimetry. The X-ray tube having titanium target was operated at 8 kV and 0.2 microA to emit Ti KX-rays of moderate intensity. The degree of the Ti KX-ray absorption, defined as similar to photographic density, was measured for the two kinds of badge film, Fuji gamma-ray badge film and Kodak personal monitoring film, type 2 exposed for 60Co or 137Cs gamma-rays and developed by the respective standard procedures. The experimental results show that the dosimetric range of 0.01-100 R for the Fuji film and 0.03-1,000 R for the Kodak film may be easily measured by 1 minute counting with the relative statistical error (sigma) of 10%.

Cesium Radioisotopes↗
PubMed1988

Immediate reconstruction following resection of oral cancer with pectoralis major myocutaneous flap.

During the past five years (August 1981 to March 1986), we applied 44 pectoralis major myocutaneous flaps (PM-MC flaps) for the purpose of intraoral and extraoral reconstruction following surgery in 43 cases of oral cancer. Out of a total 44 flaps, 30 flaps resulted in complete survival and 13 flaps resulted in partial necrosis, while complete necrosis occurred in only one flap. In addition, we used the iliac bone graft with the PM-MC flaps on five patients in order to reconstruct the mandible, but two cases failed. We also used replantation of the lower margin of the mandible on five patients, and all were satisfactory. In 26 living patients, all but one had no dysphasia. About more than a half of them had moderate or severe dysfunction of chewing. We were able to make prosthesis for five out of 26 living patients as recorded till today. We have to think about how to fashion functionable dentures on severe cases postoperatively. The results of our experiences show that the PM-MC flaps provide a simple, reliable and versatile method of primary reconstruction on patients with oral cancer.

Adult↗
PubMed1987

The effects of cortical ablation on mastication in the rabbit.

To verify the hypothesis that the control of movement by the cortical masticatory area (CMA) is particularly important at the beginning of the mastication, we compared movements before and after bilateral CMA ablation. After ablation, the animal could not manipulate food placed in the front of the mouth for several days. With further recovery, the animals could masticate in a more normal way, but the times taken to manipulate the food and to chew to the point of swallowing were increased. However, the form and amplitude of the masticatory cycles were only slightly changed by loss of cortical control. The results indicate that CMA is of great importance at the beginning of mastication and probably also in terminating chewing and initiating swallowing.

Animals↗
PubMed1987

Regulation of murine IgE production in SJA/9 and nude mice. Potentiation of IgE production by recombinant interleukin 4.

Serum IgE levels were determined in different strains of mice with enzyme-linked immunosorbent assay by using rat monoclonal anti-murine IgE antibodies in normal and in Nippostrongylus brasiliensis-infected mice. After infection, serum IgE levels were high in BALB/c and CB-20, low in SJL/J and SJA/20 mice, and not detected at all in SJA/9 and nude mice. Surface IgE-positive cells were greatly increased in BALB/c and SJL/J mice after infection, but not in SJA/9 and nude mice. Most surface IgE-positive spleen cells were also surface IgM- and surface IgD-positive. When spleen cells from SJA/9 or nude mice were stimulated in vitro with lipopolysaccharide and recombinant interleukin 4 (formerly B cell-stimulating factor 1), IgE was produced and detected in the supernatants of these cultures. In addition, surface IgE-positive cells could be detected in these cultures. Most of the surface IgE-positive cells were surface IgM- and surface IgD-negative, unlike those seen in the spleens of Nippostrongylus-infected BALB/c and SJL/J mice. These observations show that SJA/9 and nude mice have IgE-producing precursor B cells, and after appropriate stimulation interleukin 4 can induce them to secrete IgE.

Animals↗
PubMed1987

Cortically induced masticatory rhythm in masseter motoneurons after blocking inhibition by strychnine and tetanus toxin.

In the first series of experiments, we studied whether or not strychnine (STR)-resistant inhibition of masseter motoneurons (MASS . MNS) was involved in their rhythmical inhibition that occurs during masticatory activity, induced by repetitive stimulation of the cortical masticatory area (CMA) in the cat. After systemic STR injection, repetitive CMA stimulation induced rhythmically alternating activity in the masseteric and anterior digastric nerves with a shorter cycle time than before STR-administration. The short-latency IPSPS in the MASS . MNS evoked by single shocks applied to the CMA were abolished. In contrast, repetitive CMA stimulation still induced a rhythmical alternation of EPSPS and IPSPS in the MASS . MNS, although the IPSPS were significantly reduced in amplitude. In the second series, we attempted to abolish the STR-resistant component of the rhythmical IPSP with tetanus toxin (TT). This was injected into one superficial masseter muscle of the guinea pig. In the majority of animals, repetitive CMA stimulation induced a tonic EMG superimposed by rhythmical bursts in the TT-intoxicated masseter muscle. Repetitive CMA stimulation induced a rhythmical sequence of EPSPS and superimposed spikes in the MASS . MNS innervating the TT-intoxicated masseter muscle in paralyzed guinea pigs. It was concluded that: (1) the cortically-evoked short-latency inhibition of MASS . MNS is STR-sensitive, as is part of the rhythmical inhibition during CMA-induced mastication; and (2) rhythmical inhibition is not essential for the central generation of the rhythmical activity in the MASS . MNS.

Action Potentials↗
PubMed1987

Urethral obstruction owing to retroperitoneal cystic lymphangioma.

We report a case of cystic lymphangioma arising from retroperitoneal tissues of the pelvis, which caused urethral obstruction and eventual urinary retention. Sonography and computerized tomography demonstrated a retroperitoneal cystic mass in the pelvis. A preoperative diagnosis of retroperitoneal cystic lymphangioma was suggested by typical computerized and sonographic features.

Female↗
PubMed1986

Enlargement of rat submandibular salivary gland induced by single amputation of lower incisor teeth. Histological, histometric and ultrastructural studies.

Histological, histometric and ultrastructural studies of rat submandibular salivary glands (SMG) following single amputation of lower incisor teeth were made. The SMG enlargement occurred after the tooth amputation, and its maximal increase of weight was reached 7 days later. The results of histological and histometric observations showed that the enlargement of SMG was due to both hypertrophy and hyperplasia of the acinar cells, and hypertrophic acinar cells were ultrastructurally characterized by well-developed organelles such as rough-surfaced endoplasmic reticulum and Golgi apparatus which filled the entire cytoplasm. Hydropically swollen nerve endings with disappearance of vesicles were frequently found. In later stages, degenerative or necrotic cells were scattered in the acini, and the size and the structure of the remaining acinar cells became gradually similar to those of non-treated glands. The morphological changes seen in the enlarged SMG induced by single amputation of the lower incisor teeth in rat were similar in many ways to those of sialadenosis induced experimentally by active sympathomimetic drugs.

Amputation, Surgical↗