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S Fehér

Publications and source records attributed to S Fehér.

8 recordsLinked to original sources
PubMed1980

Exit routes for secretory proteins from the dog pancreas.

UNLABELLED: The relative importance of the pancreatic venous and lymphatic routes for the transport of enzymes away from the pancreas was investigated. The activities of lipase and amylase were compared in arterial plasma, pancreaticoduodenal lymph and venous plasma, pancreatic interstitial fluid and juice in 31 anaesthetized dogs. Enzyme activities were determined before, during and after stimulation of the acinar parenchyma by 4 U/kg b.w. secretin + CCK-PZ for 60 min. During stimulation either is the juice secreted into the duodenum or we ligated the main pancreatic duct. Enzyme output was calculated as the volume x enzyme activity. Initial activities of both enzymes were higher in lymph than in plasma samples. Pancreatic stimulation raised the level of both enzymes in plasma. Stimulation raised lymph lipase activity higher than in plasma, even without duct ligation. Duct ligation increased enzyme activities to considerably higher values in oedema fluid than in lymph. When enzyme output in the stimulated juice for 60 min was considered 100%, output in plasma volume (5% of b.w.) was about 15% both for lipase and for amylase during stimulated secretion, 25--30% for both enzymes when duct had been ligated. Lymphatic enzyme output was found negligible. CONCLUSIONS: during pancreatic stimulation biologically important amounts of secretory proteins enter the blood circulation. The lymphatic transport is negligible, since pancreatic lymph flow is several hundred times less than blood flow.

Amylases↗
PubMed1977

Absorption of pancreatic lipase from the duodenum into lymphatics.

A significantly higher lipase activity was measured in the duodenal lymph samples of 15 dogs than in each of corresponding arterial blood plasma samples collected prior to, during and after maximal hormonal stimulation of pancreatic secretion. The result may be evaluated as a sign of pancreatic lipase absorption by the duodenum into lymphatics.

Animals↗
PubMed1977

Humoral influences on local blood flow and external secretion of the resting dog pancreas.

Graded doses of glucagon (2--8 microgram/kg b. w.), prostaglandin E1 (PGE1) (125--1000 ng/kg b. w.), caerulein (1--32 ng/kg b. w.) and a synthetic C-terminal octapeptide of cholecystokinin-pancreozymin (CCK--PZ) 50--400 ng/kg b. w.) were infused into the superior pancreatioduodenal artery in chloralose-anaesthetized dogs to study the effect on blood flow and external secretion of the resting pancreas. Local blood flow was measured by a heated thermocouple inserted into pancreatic tissue supplied by the superior pancreaticoduodenal artery. Blood flow through this artery was measured by electromagnetic flowmeter. Juice was collected from the cannulated main pancreatic duct. Each of the four agents significantly increased pancreatic blood flow. There was a linear correlation between the doses and the blood flow responses. Caerulein and the synthetic C-terminal octapeptide of CCK--PZ increased the amount of secreted juice from the resting pancreas. The C-terminal octapeptide of CCK--PZ elevated the protein concentration of the secreted juice in proportion to the dose. Glucagon and PGE1 did not influence the amount of secreted juice from the resting gland.

Animals↗
PubMed1975

Glucagon treatment of experimental acute pancreatitis.

CFY male rats anaesthetized with pentobarbital were used in different groups for inducing acute pancreatitis by the retrograde injection either of 1 mg elastase, 5 mg trypsin, 4 mg lysolecithin, 10 mg Na-taurocholate in 0.2 ml volume or of 0.3 m. sunflower oil. In each group laparatomized animals served for control. The animals with pancreatitis were treated either with 15 mug/b.w.kg/hour glucagon or with physiological saline for 72 hours. Twenty-four and 72 hours after inducing pancreatitis glucagon did not influence the significant fall in blood pressure elicited by the intraductal injection of trypsin or elastase or in the plasma calcium level in pancreatitis induced by trypsin or sunflower oil. Neither did glucagon affect the significant increase of plasma lipase activity in pancreatitis induced by trypsin or taurocholate. It also failed to reduce the 24-hour mortality rate and the extension of fat tissue necrosis in the abdominal cavity of pancreatitic animals. In contrast, glucagon treatment significantly reduced the amount of abdominal exudate associated with bile salt induced pancreatitis and, probably due to its pancreatic blood flow increasing effect, seemed to moderate the degree of tissue damage elicited in the pancreas by detergents such as taurocholate or lysolecithin.

Acute Disease↗
PubMed1975

Effect of hypocalcaemia and lipid peroxidation on experimental acute pancreatitis.

It has been studied whether hypocalcaemia increased the mortality rate of experimental acute pancreatitis in the rat, and whether lipids injected into the pancreas underwent peroxidation, damaging thereby the gland. Prolonged hypocalcaemia was induced by bilateral parathyrodectomy and intensified by a calcium-deficient diet. Parathyroidectomy significantly decreased also arterial blood pressure. Three hours after their intraductal injection, both 0.2 ml sunflower oil and 5 mg trypsin significantly decreased arterial blood pressure in the non-parathyroidectomized animals and so did 5 mg trypsin in the parathyroidectomized ones; sunflower oil decreased the blood calcium level below 5 mg/100 ml in parathyroidectomized rats fed a calcium-deficient diet. No differences were found in the mortality rates of nonparathyroidectomized normocalcaemic rats and parathyroidectomized hypocalcaemic ones, or in the extent and gravity of pancreatic injury and in the extent of fat tissue necrosis at 3 and 24 hours after the intraductal injection of 0.2 ml sunflower oil or 5 mg trypsin. Sunflower oil, oleic acid and linoleic acid injected by retrograde way into the pancreas in 0.2 ml volume each induced extensive danage to the acinar parenchyma, while liquid paraffin failed to do so. Among these agents, only linoleic acid increased, as a sign of lipid peroxidation, malonaldehyde concentration in the pancreatic tissue. Linoleic acid injected into the submaxillary salivary gland in 0.1 ml volume induced extensive tissue damage, while sunflower oil, oleic acid failed to do so.

Acute Disease↗