Biomedical subjects

S Fei

Publications and source records attributed to S Fei.

At least 19 recordsLinked to original sources

PubMed2006

Proteomic analysis of the genotoxicant methylazoxymethanol (MAM)-induced changes in the developing cerebellum.

The genotoxicant methylazoxymethanol (MAM) is a widely used developmental neurotoxin, and its glucoside is an etiological factor for western Pacific amyotrophic lateral sclerosis-parkinsonism-dementia complex (ALS/PDC). Identification of global protein expression changes that occur in response to MAM in the developing cerebellum could provide valuable insight into the potential mechanisms involved in the neurodegeneration process. We have utilized fluorescence 2-dimensional differential gel electrophoresis (2D-DIGE), to determine the protein expression changes that occur during normal cerebellar development and in response to MAM. Three day-old postnatal C57BL/6 mice (PND3) received a single injection of MAM, and the cerebella of postnatal day 4 (PND4) and day 22 (PND22) were analyzed. Approximately, 1400 unique spots were matched and quantified in all samples. Comparison of PND4 and PND22 developing cerebellum showed that a significant fraction of the proteome (approximately 68%) changes at this stage. The immediate response of the developing cerebellum to MAM was minimal (approximately 10%). However, significant differences (27%) were noted 14 days after MAM exposure. In contrast, the transcriptome changes were more pronounced at 24 h compared to 14 days. MAM targeted several proteins networks including transport (e.g., alpha-synuclein), cytoskeletal (e.g., beta-tubulin, vimentin), and mitochondrial (e.g., Atp5b) proteins. Immunochemistry confirmed several of the changes in protein expression (alpha-synuclein). Comparison with gene expression changes revealed that the temporal changes observed in the transcriptome and proteome are not correlative. These studies demonstrate for the first time the potential networks involved during neuronal development and neurodegenerative processes that are perturbed by MAM.

Animals↗

PubMed2001

[The preliminary research of maxillary sinus cancer on oncogene level].

OBJECTIVE: To determine the oncogene c-myc, bcl-2' product expression in the maxillary sinus cancer and to investigate its clinical significance. METHOD: In 46 maxillary sinus cancer and 56 normal nasal mucous membrane, immunochemical method was adopted to determine oncogene c-myc and bcl-2' product. RESULT: In the normal nasal mucous membrane, the positive rates of oncogene c-myc and bcl-2' product were 0; in the maxillary sinus cancer, the positive rates of c-myc and bcl-2' product were 73.91 (34/46), 60.87 (28/46) respectively. The positive rate of c-myc' product in the relapse group(91.67%, 11/12) was significantly higher than that in the non-relapse group(67.65%, 23/34), P < 0.01; the positive rate of bcl-2' product in the relapse group(50%, 6/12) was significantly lower than that in the non-relapse group(64.71%, 22/34), P < 0.05; the positive rate of c-myc' product in the stage IV (88.89%, 8/9) was significantly higher than that in the stage II (63.64%, 7/11), P < 0.05; the positive rate of bcl-2' product in the stage II (72.73%, 8/11) was significantly higher than that in the stage IV (55.56%, 5/9), P < 0.05; the positive rate of bcl-2' product was in the negative correlation to the pathological grade of primary carcinoma. CONCLUSION: The oncogene c-myc and bcl-2 take part in the genesis and advancing course of maxillary sinus cancer. Their products were the important oncogology indexes, which might be useful to determine illness condition and predict relapse.

Carcinoma, Squamous Cell↗

PubMed2000

[Horizontal-vertical hemilaryngectomy for advanced laryngeal cancer].

OBJECTIVE: To evaluate the adequacy of horizontal-vertical partial laryngectomy in the treatment of advanced laryngeal cancer. METHODS: We retrospectively reviewed 57 patients with advanced laryngeal cancer (T3 or T4 on the primary site) treated by horizontal-vertical hemilaryngectomy. Fifty-six patients underwent neck dissections. RESULTS: The overall 3- and 5-year survival rates were 70% (40/57) and 64% (32/50), respectively. All patients had good phonation and resumed mouth-feeding after surgery. Among all the deaths, 4 died of recurrence of laryngeal cancer, 4 cervical metastasis, 4 distant metastasis, 2 intercurrent disease and 3 unknown. Of 36 patients with N0 neck, fifteen cases (15/36, 41.6%) had lymph node metastases pathologically. CONCLUSION: Horizontal-vertical hemilaryngectomy is satisfactory for the treatment of selected advanced laryngeal cancers. It also preserves laryngeal functions in most cases.

Adult↗

PubMed2000

[Supraglottic horizontal partial laryngectomy].

OBJECTIVE: To evaluate the long-term result of supraglottic horizontal partial laryngectomy. METHODS: Fifty-five patients with supraglottic laryngeal carcinoma were treated surgically by supraglottic horizontal partial laryngectomy from 1986 to 1995. There were 32 males and 23 females with an average age of 56. Eleven cases were staged I, 26 staged II, 11 staged III and 7 staged IV. Closure of the surgical defect begins by covering the thyroid cartilage with mucosa of the medial wall of the pyriform sinus. The outer perichondrium of thyroid cartilage was approximated with the mucosa of laryngeal ventricle. The hyoid cartilage was excised. The base of tongue was sutured to the reserved thyroid cartilage to take the place of epiglottic cartilage. RESULTS: The 3- and 5-year survival rates were 85% (47/55) and 77% (37/48) respectively. The decannulation rate was 96%. Most cases had acquired good speech and swallow function after 8-30 days. Complications included granulation and polyp formation at the base of tongue or at the anterior commissure in 4 cases. CONCLUSIONS: Supraglottic horizontal partial laryngectomy is very effective both in eradicating disease and in preserving laryngeal function.

Adult↗

PubMed2000

[The study of p16 gene mutation and methylation in laryngeal squamous cell carcinoma].

OBJECTIVE: To investigate the development and mechanism of the p16 gene inactivation in laryngeal squamous cell carcinoma (LSCC). METHODS: With PCR-based methylation assay and PCR-SSCP, first and second exon mutation in p16 gene and methylation of endogenase in promoter in 32 cases of LSCC and paracancer tissue were studied. RESULTS: No mutation were found in first and second exon in the samples; methylation of Cfo I were detected in 6 cases in first exon, methylation of Sac II was found in 4 cases, and methylation of Hpa II in 4 cases. In second exon, methylation of Hpa II was detected in 5 cases and methylation of Cfo I in 6 cases. CONCLUSION: Methylation of promoter in p16 gene is one of the important mechanisms of the inactivation of the gene. The inactivation of p16 gene has a close relation with the development and progress of LSCC.

Carcinoma, Squamous Cell↗

PubMed2000

[Long-term follow-up result of partial laryngectomy].

OBJECTIVE: To study the long-term follow-up result of partial laryngectomy and reservation of laryngeal function. METHODS: Three hundred and seventy-nine patients who underwent partial laryngectomy from 1986 to 1995 were summarized (male 290 cases, female 89 cases). Among them, 184 cases were supraglottic carcinomas (T1 8 cases, T2 115, T3 48, T4 13, according to UICC in 1992), 192 cases were glottic carcinomas (T1 115, T2 63, T3 13, T4 1), 3 cases were transglottic carcinomas (T2 1, T3 2). In common 8 kinds of operations were performed: 26 cases underwent cordectomy, 138 vertical laryngectomy, 7 frontolateral laryngectomy, 12 horizontal glottic laryngectomy (middle part of the larynx), 58 supraglottic laryngectomy, 95 horizontovertical (3/4) laryngectomy, 24 subtotal laryngectomy with cricoglossoepiglottic anastomosis, 19 near-total laryngectomy with cricoglossal anastomosis (with reservation of unilateral arytenoid cartilage). 193 cases underwent concurrent neck dissection(121 unilateral, 72 bilateral). RESULTS: All cases restored their phonation and overcame aspiration with removing nasal feeding from 7 to 23 days after operations. 362 cases were decannulated from 9 days to 3 months after operations. Another 8 cases were decannulated after a secondary plastic operation. Decannulation rate was 97.6%. The three, five and ten year survival rates were 86.8% (329/379), 81.3% (266/327) and 69.4% (120/173) respectively. CONCLUSION: Partial laryngectomy is a kind of radical operation with reservation of laryngeal function. Mastering indications strictly, correct operation choices, excellent surgical skills and perfect repairing technique are bases of improving life qualities and curative effect.

Adult↗

PubMed1999

Interaction of paratropomyosin with beta-connectin and its 400-kilodalton fragment from chicken skeletal muscle as influenced by the calcium ion concentration.

The binding of paratropomyosin to beta-connectin, which has been suggested to interact at the A-I junction of a sarcomere, was confirmed by measuring the changes in turbidity of a mixture with changing NaCl concentration, pH and free calcium ions, and by morphological observation and a coprecipitation assay of the aggregates formed in the mixture. Paratropomyosin also bound to the 400-kDa fragment which is the N-terminal portion of beta-connectin and contains the A-I junction region. Moreover, the interaction of paratropomyosin with the 400-kDa fragment was enhanced by a calcium ion concentration from 10(-7) M to 10(-5) M and markedly suppressed above 10(-4) M calcium ions. We conclude that paratropomyosin probably binds to the 400-kDa fragment of beta-connectin in the A-I junction region in living and pre-rigor skeletal muscle. In postmortem skeletal muscle paratropomyosin may be released from the 400-kDa portion of the connectin filament by increased calcium ion concentration and translocated on to thin filaments to induce meat tenderization.

Animals↗

PubMed1999

[The histopathological observation and the clinical significance of invasion of pyriform sinus apex in pyriform sinus carcinoma].

OBJECTIVE: To approach the clinical significance of pyriform sinus carcinoma invading to pyriform sinus apex. METHOD: Forty-three cases of surgical specimens from total laryngectomy and partial hypopharyngectomy of pyriform sinus carcinoma were made with the organ subserial sectioning and pathological study. RESULT: Pyriform sinus apex is the inferior 1/3 region of pyriform sinus. If pyriform sinus carcinoma invades its apex, the cancerous tissue easely infiltrated the adjacent structure and organs. CONCLUSION: The invasion of pyriform sinus apex is the beginning position and the most important sign of invading downward the tissue and surrounding organs in the inferior position of hypopharynx. When surgery treatment is performed, the total laryngectomy and hypopharyngectomy of ill hypopharynx is appropriate and necessary.

Adult↗

PubMed1999

[Long-term follow-up result of partial laryngectomy in senile patients with laryngeal carcinoma].

OBJECTIVE: To study the long-term follow-up result of laryngectomy with reservation of laryngeal function in senile patients. METHODS: Ninety-nine patients who were above 65 years old and underwent partial laryngectomies from 1986 to 1990 were summarized. Among them, 14 cases underwent vocal cord resection, 31 vertical hemilaryngectomy, 15 horizontal supraglottic hemilaryngectomy, 2 horizontal glottic laryngectomy, 22 horizontovertical (3/4) laryngectomy, 5 subtotal laryngectomy, 10 total laryngectomy with cricopharyngeal anastomosis. RESULTS: All patients had their phonation and respiration restored. Decannulation rate was 91.9%. No death occurred during the operation. No severe postoperative complications were found. 3.5-year survival rates were 76.7% and 75.5% respectively. CONCLUSION: Senility is not a contraindication for laryngectomy with reservation of laryngeal function. The key points are that surgeons should master indications correctly and improve surgical skill to decrease postoperative complications.

Aged↗

PubMed1998

Binding of paratropomyosin to beta-connectin from chicken skeletal muscle.

We found that paratropomyosin bound to beta-connectin, in examining binding of paratropomyosin at the junction of A- and I-bands of sarcomeres. The turbidity of a mixture of beta-connectin and paratropomyosin was greater with more paratropomyosin added, but high concentrations of Ca2+ suppress this increase. These results suggest that paratropomyosin is released from connectin filaments at the A-I junction region by increased concentrations of calcium ions in postmortem skeletal muscles.

Animals↗

PubMed1998

[Transglottic carcinoma and transglottic invasion of laryngeal carcinoma].

OBJECTIVE: To explore the definition of transglottic carcinoma and its pathological characteristics of local invasion, and to determine the distinction between transglottic carcinoma and transglottic invasion of laryngeal carcinoma. METHODS: Fifty total laryngectomy specimens of transglottic carcinoma were subject to whole-organ serial section, HE staining and microscopic observation. RESULTS: The ventricles of 50 cases were all invaded, the main lesion of carcinoma was in the ventricle, or the ventricle was the center of the lesion. The rate of invasion to paraglottic space(PGS) was 82%(41/50). Submucosal invasion was seen in 52%(26/50) and that with superficial mucosal invasion was seen in 38%(19/50). Superficial mucosal invasion rate was 10%(5/50). Deep submucosal invasion was the major way of tumor spread in transglottic carcinoma which equally involved the supraglottic and glottic regions. CONCLUSION: Transglottic carcinoma originates from the center of ventricle where it spreads to transglottic region. Submucosal extension is characteristic of local invasion. There are fundamental differences between transglottic carcinoma and transglottic invasion of laryngeal carcinoma in the late stage of the lesion.

Adult↗

PubMed1998

[Detection of human papillomavirus in Chinese laryngeal carcinoma with digoxigenin labelled probe prepared by PCR].

The studies described here detect HPV DNA in 124 cases of fresh tissue samples with different lesions of larynx using a nonradioactive Digoxigenin Labelled Probe prepared by polymerase chain reaction (PCR). The labelled probe of HPV consensus primers was obtained, which can detect nine types of HPVs DNAs such as HPV 6, 11, 16, 18, 31, 33, 35, 42 and 58. The results showed that the rate of positive cases with HPV infection was 45.6% (31/68) in the group of laryngeal carcinoma, 20.0% (3/15) for the group of neck metastatic lymph node, 11.8% (2/17) for precarcinous lesions group and 6.3% (1/16) for the group of vocal cord polypus, respectively. Fifteen cases of normal laryngeal tissue adjacent to the carcinoma and 15 cases adjacent to the carcinoma were HPV DNA negative. The carcinogenic action of HPV in laryngeal carcinoma and the sensitivity and specificity of the probe prepared by PCR are discussed in this paper.

DNA, Viral↗

PubMed1998

[Loss of heterozygosity on chromosome RB1.20 locus and p110RB1 protein state in squmous cell carcinomas of larynx].

OBJECTIVE: To study the RB1 gene in squamous cell carcinoma of larynx(ISCC) and to find clue for discovering and locating new suppressor gene. METHODS: Loss of heterozygosity (LOH) of microsatellite polymorphic sequence on chromosomes 13 at RB1.20 locus of 58 LSCC patients were analyzed. The p110RB1 protein state was detected by immunohistochemical staining using the polyclonal antibody to the RB1 products. RESULTS: It showed that 3 cases in the preinvasive stage (i.e. carcinoma in situ) had no any LOH on chromosome 13. Forty percent of the 55 invasive LSCC showed LOH at RB1.20 locus. By immunohistochemical staining p110RB1 protein negative reaction was observed in 8 LSCCs in which 6 cases associated with LOH at RB1.20 locus. CONCLUSIONS: RB1 gene was inactivated in some LSCCs. It is putative that there is another tumor suppressor gene on chromosome 13 near by RB1.20 locas. The inactivation of the genes at the chromosome 13q region including RB1 involved genesis and development of invasive LSCC.

Carcinoma, Squamous Cell↗

PubMed1997

[Laser assisted uvulopalptoplasty treatment for obstructive sleep apnea syndrome].

71 patients of obstructive sleep apnea symdrome (OSAS) were treated by Nd:YAG laser assisted uvulopalptoplasty (LAUP). According to the atonamic features of every patient, and type of operation which should be selected, the success rate of surgery would increase, and complications would be reduced. The effect rate is 97.2% by clinic, 90.0% by polysimnograph (PSG).

Adult↗

PubMed1997

[Histopathological study of local invasion and cervical lymph node metastasis in transglottic carcinoma].

To study the histopathological characteristics of local invasion and cervical lymph node metastasis in transglottic carcinoma and provide theoretical guide to clinical practice, 30 laryngectomy and 35 neck dissection specimens were studied by serial section, HE staining and observation under light microscope. The results showed that transglottic carcinomas originated from the ventricle invaded supraglottic and glottic areas equally. The metastatic rate of cervical lymph nodes was 43.3%; the latent metastatic rate was 30%. The metastatic and extracapsular perforation rates as well as the number of the aggregated fusion lymphnodes increased with the increase in the size of the lymphonodes. It was concluded that the transglottic carcinoma should be defined as one with the main lesion locates in the ventricle, or the ventricle being the center with transglottic spread. Since the barrier of ventricle was weak, partial laryngectomy should be performed with care, and ipsilateral neck dissection shoud be included during operation.

Adult↗

PubMed1997

[A report of 50 cases of transglottic carcinoma].

50 cases of transglottic carcinoma were chosen and the collodion-embedded specimens of whole-organ serial section were observed. We found that: 1. Transglottic carcinoma was of laryngeal ventricle origin; 2. There were 1 case in T1 and 2 cases in T2 category; 3. Deep infiltration was the main local growth pattern (90%, 45/50), and paraglottic space and thyroid cartilage were easily involved (82%, 41/50 and 65%, 32/50 respectively), clinical stage was not parallel to pathological stage (48%, 24/50).

Adult↗

PubMed1997

[On the mechanism of hemilaryngeal fixation in pyriform sinus carcinoma and its clinical significance].

To approach the mechanism of hemilaryngeal fixation in pyriform sinus carcinoma (PSC), surgical specimens from total laryngectomy and partial hypopharyngectomy in 43 cases of pyriform sinus carcinoma were studied using organ subserial sections. Invasion of intralaryngeal muscles and the upper and outer aspects of arytenoid cartilage via paraglottic space was found to be the main cause of hemilaryngeal fixation in these cases. Presence of tumor on the medial wall of the pyriform sinus makes involvement of intralaryngeal structures easy, and thus is more frequently seen with hemilaryngeal fixation. Clinical T3 lesion of the sinus may infiltrate laryngeal cartilages and adjacent soft tissue to become pathologic T4 lesion. For PSC appearing hemilaryngeal fixation, total laryngectomy is safer to thoroughly remove cancer.

Adult↗

PubMed1996

[Relationship between voice spectrum analysis and psychoacoustic evaluation in patients with vocal cords polyps].

The purpose of this paper is to investigate the significance of selected acoustic parameters, to determine their usfulness in monitoring effects of treatments, to correlate them to other clinical voice tests (such as aerodynamic measurements, stroboscopic examination, psychoacoustic evaluation), and to determin if there were correlations among the acoustic parameters. The results showed that pitch perturbation quotient (PPQ), amplitude perturbation quotient (APQ), normalized noise energy a and b (NNEa and NNEb) were positively related to the regularity (REG) in the vocal cord polyp. In the present study, psychoacoustic rough (R), breathy (B) were correlated to the regularity (REG), glottic closure (GLO) and the mean airflow rate. The integrated voice analysis was a simple and effective method for acoustic evaluation of pathologic voice.

Adult↗