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S Fligiel

Publications and source records attributed to S Fligiel.

25 records · Page 2Linked to original sources
PubMed1982

Disseminated Mycobacterium avium-intracellulare infection in homosexual men dying of acquired immunodeficiency.

Five homosexual men dying at UCLA Center for the Health Sciences, Los Angeles, with acquired immunodeficiency and Pneumocystis pneumonia, Kaposi's sarcoma, or cryptosporidiosis since May 1981 have all had mycobacteria of the Mycobacterium avium-intracellulare complex cultured from tissues taken just before death or at postmortem examination. Each man had histological evidence of disseminated mycobacterial infection. Acid-fast organisms were seen in macrophages in the lung, spleen, and lymph nodes in all cases and in a variety of additional organs in two cases. Other severe infections were always found at postmortem examination--cytomegalovirus, cryptosporidiosis, and Pneumocystis. Disseminated M avium-intracellulare infection has been so striking in homosexual males dying with acquired immunodeficiency at our institution that we believe a vigorous search for mycobacteria should be made in all such patients. Empiric therapy for mycobacterial infection may be justified in selected cases of immunodeficiency before a specific microbiological diagnosis.

Acquired Immunodeficiency Syndrome↗
PubMed1982

Glucocorticoids and melanoma: receptor properties of dexamethasone sensitive and resistant tumors.

We have grown solid tumors using dexamethasone-sensitive (clone 6) and -resistant (clone 5) cells cloned from RPMI 3460 Syrian hamster melanoma. Clone 6 but not clone 5 tumor growth was retarded by dexamethasone, indicating that these tumors retain the growth sensitivity to the hormone characteristic of the cells from which they are derived. Both tumor types contain cytosolic glucocorticoid receptors (significantly higher levels in clone 5 tumors) with similar affinity and steroid specificity characteristics and these can exist as stable, activated (nuclear binding) complexes. Despite these similarities the receptor in the two tumor types differ by some physiochemical criteria. By sucrose gradient analysis, cytosols from both tumors contain 7S receptor complexes but clone 6 contains an additional 13S form. Activated receptors isolated by DEAE-cellulose chromatography from both clone 5 and 6 tumor cytosols sediment as a single peak at 4-5S. However, the DEAE-cellulose profiles indicate that clone 6 but not clone 5 activated complexes (Peak I) appear heterogeneous with respect to charge. Interestingly, DNA-cellulose chromatography indicates that activated receptors from clone 5 tumor cytosols may bind more tightly to DNA than those from clone 6. We are investigating these receptor differences in more detail to determine more precisely the role and pathways of action of glucocorticoid hormones in melanoma.

Animals↗
PubMed1982

Necrotizing vasculitis in a case of disseminated neonatal herpes simplex infection.

A term newborn suffered disseminated herpes simplex virus (HSV) type II infection five days after cesarean section delivery for fetal distress. The mother had no history or evidence of herpetic lesions; the father had a history of genital herpetic lesions. The infant's terminal course was dominated by disseminated intravascular coagulation (DIC) with hepatic and renal failure. Microscopic examination revealed a necrotizing vasculitis of small and medium-sized lung and peripancreatic arteries. Nuclear inclusions characteristic of HSV were found in these arteries, as well as in the adrenal parenchyma, spleen, and lymph node; electron microscopy confirmed replication of virus within the arterial endothelial cells. The mechanism of arterial damage in severe herpetic infection contrasts with the immune-complex mechanism postulated for other viral vasculitides. Direct, virally induced arterial damage resulting in exposure of collagen may set the stage of DiC, a commonly fatal complication of this disease.

Arteries↗
PubMed1982

Xanthogranulomatous cholecystitis: case report and review of the literature.

We studied a case of xanthogranulomatous cholecystitis, an entity rarely described in English-language literature. Our lesion is unique with regard to the extent of involvement. It is characterized grossly by a markedly thickened gallbladder wall that is yellowish and granular. Microscopically, the gallbladder was diffusely infiltrated by foamy macrophages that contained lipofuscin, lipid material, and bile pigment, features corroborated by transmission electron microscopy. The origin and pathogenesis of xanthogranulomatous cholecystitis was reviewed. We postulate that, in the presence of obstruction and bile stasis, the combination of inflammation, tissue necrosis, and bile extravasation will lead to formation of xanthogranulomas similar to that produced in experimental pyelonephritis.

Cholecystitis↗
PubMed1981

Epithelial characteristics of five subpopulations of a heterogeneous strain BALB/cfC3H mouse mammary tumor.

We have described previously the isolation and characterization of five distinct subpopulations of tumor cells from a single spontaneous strain BALB/cfC3H mouse mammary tumor (Cancer Res., 38: 3174--3181, 3758--3763, 1978). Subpopulations 68H and 4.10 are polygonal and grow in epithelioid patterns in vitro, whereas subpopulations 66, 67, and 168 are fusiform and grow in lattice or fibroblast-like patterns. Line 4.10 produces tumors with distinctly glandular architecture, whereas the other four subpopulations produce poorly differentiated tumors with mixed epithelial-sarcomatous histological patterns. All five lines were evaluated for epithelial characteristics. Dome formation, characteristic of transporting epithelial cells, could be induced by dexamethasone or dimethyl sulfoxide only in line 4.10 cells. Antibodies to cell type-specific mammary epithelial antigens reacted with each of the subpopulations. All five subpopulations had ultrastructural features of epithelial cells, including desmosomes (all five lines), junctional complexes (68H, 4.10, early-passage 66 and 67 only; poorly defined in 168), and growth in cords demonstrating polarity (68H cells). Less definitive myoepithelial characteristics were also seen in four of the lines, including an incomplete reaction for Na+-K+-ATPase (4.10 cells), hemidesmosome-like junctions (168 and early-passage 66 cells), and pinocytotic vesicles at lower than normal frequency (66, 67, and 168 cells). Thus, none of the lines were distinctly myoepithelial. We conclude that the five subpopulations are epithelial cells that express a spectrum of epithelial characteristics.

Animals↗