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Biomedical subjects

S Gál

Publications and source records attributed to S Gál.

16 recordsLinked to original sources
PubMed1997

Primary resection with antegrade colonic irrigation and peritoneal lavage versus subtotal colectomy in the management of obstructed left colon cancer.

Between 1980 and 1996 122 patients with acutely obstructed resectable carcinomas of the colon and rectum were treated in our hospital. Ninety-four has undergone one stage operation of immediate resection and primary anastomosis without proximal colostomy. Intraoperative colonic irrigation was performed in 34 patients, subtotal/total colectomy in 30 patients, right hemicolectomy in 30 patients. There were 2.3 and 1 operative deaths, respectively. The average hospital stay for the survivors was 19.8 days in the primary resection and anastomosis group, and 41.0 days in staged operation group. Concerning the possibility of multiple lesions the authors recommend subtotal/total colectomy except for tumours localized in the sigma where they apply total colectomy only when the proximal part of the colon is necrotized or filled with solid stool.

Acute Disease↗
PubMed1991

Use of chromatofocusing for separation of beta-lactamases. IX. Analytical chromatofocusing for the separation of a chromosomal cephalosporinase from Proteus vulgaris 1028.

Simultaneous purification and isoelectric point (pI) determination was carried out at analytical scale of the chromosomal cephalosporinase from the Proteus vulgaris 1028 strain. Comparison of the enzyme to the purification results with m-aminophenylboronic acid-agarose affinity chromatography with sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed that minute amounts of accompanying proteins having identical pI values but different molecular masses were found in the chromatofocused preparation. The molecular mass of the enzyme was 24,000 dalton. The pI was found to be 8.3.

Cephalosporinase↗
PubMed1991

[Sphincter-saving procedure of abdomino-peritoneal amputation? Surgical management of cancer of the lower two third of the rectum].

UNLABELLED: The authors report their results in surgical treatments of carcinoma localized in the low two thirds of the rectum done between 1980 and 1988. The number of patients was 150. Resectability: 119/150 = 79.33 per cent. Lethality: 4/119 = 3.3 per cent. Seventy three of the patients (61 per cent) had sphincter saving procedures and 46 of them (38.7 per cent) had abdominoperineal excision. Out of the 64 low anterior resection 2 patients (L: 2/64 = 3.1 per cent) and out of the 46 abdominoperineal excision also 2 patients (L: 2/46 = 4.5 per cent) were lost. In the sphincter saving group the distal clearance margin was decreased to below 3 cms at 29 patients without having local recurrence. CONCLUSION: in many cases of the carcinoma localized in the middle third of the rectum (at 82 per cent of our own patients) the sphincter can be saved without having more local recurrences. Decreasing the distal clearance margin to 2.5 cms does not increase the possibility of local recurrence if we do it cranially the same way as at Miles operation and remove the mesorectum caudally and laterally.

Anal Canal↗
PubMed1988

Modified general affinity adsorbent for large-scale purification of penicillinases.

N-Acetyl-D-(-)-penicillamine as a stable second-generation biospecific affinity ligand has previously been suggested for purification of Bacillus cereus 569/H beta-lactamase I. A complex spacer arm is coupled with the matrix by using epichlorohydrin and phloroglucinol doubly activated with divinyl sulphone in the meta position. Coupling of D-(-)-penicillamine ligand resulted in an active affigel. However, we found that two affinity ligands in close proximity prevents simultaneous binding of two penicillinase molecules, therefore one ligand is superfluous. Our results show that: (1) shortening the spacer arm by direct activation of the matrix with divinyl sulphone is satisfactory to produce the affinity material with N-acetyl-D-(-)-penicillamine; (2) incorporation of 15 mumol of N-acetyl-D-(-)-penicillamine per ml of wet Sepharose 4B satisfies the maximum binding capacity requirements of the affigel (about half of the originally incorporated amount of ligand); (3) our simplified affinity adsorbent is generally applicable for large-scale purification of penicillinases to homogeneity from various bacterial sources by the convenient batch method without prior concentration of these enzymes; (4) reacetylation for four/five times can regenerate the original binding capacity of the affigel.

Bacteria↗
PubMed1987

Use of chromatofocusing for separation of beta-lactamases. VIII. Analytical chromatofocusing of chromosomal cephalosporinases from four Klebsiella strains.

Although still there are Klebsiella strains which do not harbour plasmids and produce constitutive chromosomal beta-lactamases, recently clinical isolates were found in ever increasing numbers carrying mainly TEM-, CARB- and OXA type R-factors. We selected four chromosomal cephalosporinase producing Klebsiella strains to study the pI values of the enzymes and their simultaneous separability from accompanying proteins by chromatofocusing techniques. We compared pI values of the pure and the crude preparations: K. pneumoniae K1 SC 10436: pIpure = 6.4, pIcrude = 6.42; K. aerogenes K1 1082 E: pIpure = 6.5, pIcrude = 6.5; K. oxytoca 1082 E: pIpure = 6.42, pIcrude = 6.4; K. oxytoca 20: pIpure = 7.62, pIcrude = 7.6. Excellent agreement of the pI values among each other, but occasional differences with those obtained by analytical isoelectrofocusing are attributed to methodological diversities and to the presence of satellite enzymes, known to exist in Klebsiella.

Bacterial Proteins↗
PubMed1981

Changes in the physical behavior of low density lipoprotein in the presence of glycosaminoglycans and high density lipoprotein.

Temperature dependent techniques - differential scanning calorimetry (DSC), polarizing microscopy - were used to study the properties of human serum low density lipoprotein (LDL) and its glycosaminoglycan (GAG) complexes, and to investigate the influence of the addition of high density lipoprotein (HDL) to the complex system. In the LDL molecule a reversible endothermic transition took place with its peak at 33 degrees C. Cholesteryl esters within the LDL core existed as an isotropic solution above this temperature (i.e. around body temperature), and in the form of smectic liquid crystals below it. When LDL was converted in vitro into GAG-LDL complexes by the addition of chondroitin-6-sulfate, dermatan sulfate, heparin or heparan sulfate, the DSC curves showed an evaluation of the transition temperature: the peak values in these samples were found at 40 degrees C and under the polarizing microscope a birefringence developed, typical of smectic liquid crystals. In chondroitin-4-sulfate-LDL complexes no alteration of the physical structure of the LDL molecule could be demonstrated. HDL decreased the transition temperature of GAG-LDL complexes and the disappearance of birefringence indicated that in the presence of HDL the lipids within the GAG-LDL complexes existed in a liquid phase at a temperature in the vicinity of body temperature.

Calorimetry, Differential Scanning↗