Biomedical subjects

S Głab

Publications and source records attributed to S Głab.

9 recordsLinked to original sources

PubMed2001

Application of Prussian blue-based optical sensor in pharmaceutical analysis.

Optical flow-through cell-detector with incorporated transparent chemosensitive layer of Prussian blue has been applied in simple, single-channel flow-injection system for pharmaceutical analysis. The reductant analyte converts the Prussian blue based sensing layer to Prussian white form, and the attendant color change is used for sensing. Discoloration of the film is spectrophotometrically detected at 720 nm wavelength. The flow injection system has been successfully used for selective determination of ascorbic acid in simple and complex pharmaceuticals. The method is free from interferences caused by various ions and active ingredients commonly found in pharmaceuticals. The flow-through sensor is useful for spectrophotometric flow-injection analysis of intensively colored and turbid samples. The results of medicine analysis are comparable to those obtained using reference pharmacopeal method. The analytical system could be also used for determination of cysteine and hydrogen peroxide in medicines.

Ascorbic Acid↗

PubMed2001

Optical biosensors based on Prussian Blue films.

Optical biosensing schemes based on enzymatically modified inorganic/organic transparent films predominately composed of Prussian Blue are demonstrated. The composite film, which is non-electrochemically deposited on a non-conducting support. is used as an optical transducer for flow-through biosensors based on hydrolases and oxidases. Urease and glucose oxidase are utilized as model enzymes. Action of the urea biosensor is based on optical pH sensitivity of Prussian Blue indicator. The glucose biosensor is acting as first-generation optical biosensor based on in situ generated Prussian White transducer for hydrogen peroxide. These simple, single-pass transmission optical biosensors exhibit sensitivity in the millimolar range of concentration. The biosensors are very stable owing to presence of a poly(pyrrolylbenzoic acid) network in the composite material. This organic polymer plays a dual role as a binding agent for inorganic material and as a functionalized support for strong covalent immobilization of enzyme molecules.

Journal Article↗

PubMed2000

[Evaluation of methicillin-resistance in Staphylococcus aureus by the agar disk diffusion method and PCR].

Staphylococcus aureus is a widespread human pathogen. One the most striking characteritics of this bacterium is resistance to methicillin and all beta-lactam antibiotics. The agar disk diffusion method is the most widely used in vitro susceptibility test, but recently molecular methods, e.g. Polymerase Chain Reaction, have been also introduced. We compared the detection of methicillin resistant coagulase positive Staphylococcus aureus isolated from clinical materials in Silesian microbiological laboratories by diffusion method and PCR through the detection of nuc and mec A genes. Our results show that PCR used for the detection of mec A gene increases the detection of methicillin-resistant Staphylococcus aureus strains by 10% as compared to the agar disk diffusion method. Among Staphylococcus aureus strains, detected as methicillin-resistant, 17% of organisms showed no presence of mec A gene.

Colony Count, Microbial↗

PubMed1996

[Puerperal infection of the urinary tract].

An urine routine and bacteriological analysis was performed in 100 female patients in third "trimester" of pregnancy and in the second day after delivery. When bacteriuria was detected a next urine bacteriological examination was done in 8-10 and 30-50 days after the delivery. A rate of bacteriuria in female patients before the delivery was 33% and after 56%. The results of routine urine analysis were in normal limits in 31.1% of females with significant bacteriuria. A spontaneous disappearance of bacteriuria between 8 and 50 day after the delivery was observed in 46.6% of patients, but in 53.4% symptoms of urinary tract infection were noticed. Escherichia coli (serotype A and B) was defined in 79.3% of observed urinary tract infections.

Adult↗

PubMed1981