MEASLES VACCINATION. IV. RESPONSES TO TWO DIFFERENT TYPES OF PREPARATIONS GIVEN AS A FOURTH DOSE OF VACCINE.
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Biomedical subjects
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The demonstration of immunological differences between poliovirus strains of any one type is a valuable procedure in epidemiological research as it may allow a virus strain to be identified as derived from or unrelated to a given possible source of infection. It is obviously of particular importance in connexion with live poliovirus vaccination campaigns. Both kinetic tests and conventional neutralization and complement-fixation techniques have been used to this end, the former involving a more complicated test procedure and the latter demanding greater nicety in the pre-standardization of reagents. The present paper reports on attempts to establish a simplified technique.Neutralization titres of sera obtained by immunization of guinea-pigs with three strains of type 1 poliovirus (including one isolated from a patient in the 1958-59 epidemic in Léopoldville described in the two preceding papers) indicated a degree of strain specificity sufficient to permit the design of a simple screening method for the purpose of a rough immunological classification.Preliminary observations on isolates from persons fed attenuated virus indicate that antigenic changes may occur in the course of multiplication of the virus in the human intestinal tract.