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Biomedical subjects

S Gallet

Publications and source records attributed to S Gallet.

At least 19 recordsLinked to original sources
PubMed2005

Simple and rapid method for cis- and trans-resveratrol and piceid isomers determination in wine by high-performance liquid chromatography using chromolith columns.

A simple and rapid HPLC method has been developed for simultaneous determination of the four resveratrol forms (aglycon and glycosidic) in a Grenache wine from Châteauneuf du Pape (Vaucluse). These analyses were achieved by using two commercial monolith HPLC columns and diode array detection. The method provided reliable separations at low pressure with a short analysis time. The limit of detection (LD) and limit of quantification (LQ) were calculated for each standard. The molecules were separated and quantified in a single run without any purification of the sample.

Chromatography, High Pressure Liquid↗
PubMed1999

Failure to demonstrate a direct caroticocavernous fistula due to arterialisation of the ipsilateral cavernous sinus.

We report a woman in whom a small direct caroticocavernous fistula (DCCF) was revealed after successful transarterial occlusion of a contralateral fistula which drained into both cavernous sinuses. We underline that a second smaller fistula can be masked by a contralateral larger one due to the lack of pressure gradient between the internal carotid artery (ICA) and arterialised blood in the ipsilateral cavernous sinus. We suggest that bilateral ICA angiograms should be performed before and after occlusion of all DCCF.

Aged↗
PubMed1997

On the use of ion-pair chromatography to elucidate doxorubicin release mechanism from polyalkylcyanoacrylate nanoparticles at the cellular level.

The major hypothesis underlying the remarkable efficiency of polyalkylcyanoacrylate particles loaded with doxorubicin against multidrug resistant tumor cells in vitro, is based on the ion-pair association of doxorubicin with soluble hydrolysis products of polyalkylcyanoacrylate. In an attempt to demonstrate the validity of this hypothesis, we have used ion-pair reversed-phase high-performance liquid chromatography and a laboratory-synthetized compound, i.e., the 2-cyano-2-butylhexanoic acid, as a model for polyalkylcyanoacrylate highly polydispersed degradation products. It is shown that, compared to a counter-ion, like heptane sulfonic acid, 2-cyano-2-butylhexanoic acid exhibits an effective ion-pairing effect at different pH values and organic mobile phase conditions. Moreover, at pH close to physiological conditions and at low mobile phase organic modifier percentage, this effect is experimentally observed, which strongly supports the initial hypothesis.

Antibiotics, Antineoplastic↗
PubMed1997

Sedimentation field-flow fractionation of cellular species.

More than 15 years ago a pioneering report on the separation of cellular material using sedimentation field-flow fractionation (SdFFF) was published. Surprisingly, only a few reports on SdFFF applications to cell separations are available as yet. The major limitations which seemed to slow the development of SdFFF applications in biology appeared to be related to the development of specific instrumentation. Therefore, guidelines are given for setting up a biocompatible SdFFF apparatus. SdFFF elution of cells with different characteristics was performed to demonstrate the efficiency, completeness, and rapidity of cell separations by this method. Retention data describing the effect of lifting forces on nucleated cells are compared to those for red blood cells (RBC). The effects of flow rates, field intensity, and injection protocol were studied using normal human RBC eluted in isotonic medium as a probe for retention conditions. When possible, data were also compared to previously published reports. Guidelines for elution and separation optimization are given. Using mixtures of nucleated and living red blood cells, viability studies showed a surprisingly high recovery of each type of material.

Cell Fractionation↗
PubMed1996

[Neonatal rhinopharyngeal obstruction due to craniopharyngioma].

BACKGROUND: Craniopharyngioma, the most common tumor of the sellar and suprasellar regions in childhood, can exceptionally develop in the infrasellar area. CASE REPORT: A premature girl (GA: 30 weeks) weighing 1240 g required intubation and ventilation because she suffered from a neonatal respiratory distress syndrome. Nasal endoscopy showed total obstruction of rhinopharynx by a tumor resembling adenoid tissue. This tumor was successfully excised and its histological examination showed a craniopharyngioma. CONCLUSION: A wide range of intranasal tumors has to be examined, endoscopy and CT scan are necessary for a precise localization and to eliminate meningocele.

Craniopharyngioma↗
PubMed1989

[LHRH in the human hypothalamus. Immunohistochemical mapping in normal newborn infants or in sudden death infants].

The topographical distribution of neurons containing LHRH has been investigated in newborn hypothalamus using the peroxidase anti-peroxidase technique. In control subjects, LHRH immunoreactive (LHRH-IR) perikarya have been mainly observed essentially in the infundibular nucleus. The preoptic region displayed a moderate density of LHRH-IR cell bodies. High LHRH innervation was observed in the anterior hypothalamus in the lamina terminalis and in the mediobasal hypothalamus in the median eminence, and in the peri- and paraventricular regions. In sudden death infant syndrome, a comparable mapping was observed, except a low density in the mediobasal peri- and paraventricular areas.

Brain Mapping↗
PubMed1989

[Ulcero-necrotizing enterocolitis: the role of cytomegalovirus. Apropos of a case].

The authors report a case of Wilson-Mikity syndrome in a preterm newborn, that was followed on the 28th day of life by a necrotizing enterocolitis with colic stenosis. The finding of cytomegalic cells on the pathologic examination of the intestinal lesions, the presence of the virus on direct examination of a tissue sample, the elevated IgG levels and the presence of IgM demonstrated a cytomegalovirus infection. The authors discuss the role of this virus in the pathogenesis of the affection, either as directly responsible for the enterocolitis or as a secondary colonization of previous lesions.

Colectomy↗
PubMed1989

[Which treatment for primary tuberculosis?].

Primary tuberculosis should be treated in all cases. In adults, the total treatment course for pulmonary tuberculosis can be shortened to 6 months. If the disease is not symptomatic, the rifampicin-isoniazid combination (10 mg/kg/day each) appears to be preferable to single drug therapy. In patients with symptomatic disease, pyrazinamide (30 mg/kg/day) should be added during the first 6-8 weeks and in some cases, corticosteroid therapy is also required. The drugs are usually well tolerated, but hepatic function should be monitored.

Acquired Immunodeficiency Syndrome↗
PubMed1986

[Neonatal bilateral interstitial emphysema. Surgical treatment. Apropos of 1 case].

The authors report a case of respiratory distress secondary to inhalation of meconial amniotic fluid treated by mechanical ventilation, which was complicated by severe interstitial emphysema. Treatment by pleurotomy allowed a favorable evolution. The various therapies of interstitial emphysema of the neonate, in particular surgical technics are described.

Humans↗
PubMed1985

[Acute endocarditis caused by Kingella kingae in an infant].

A case of bacterial endocarditis in a one year-old boy is reported. There was no underlying heart disease. The organism was a Kingella kingae, an aerobic Gram negative bacillus, a normal inhabitant of the upper respiratory tract. It has rarely been implicated as a pediatric pathogen. Occasionally it can cause bone and joint infections and exceptionally endocarditis.

Acute Disease↗
PubMedDate not supplied

[Continuous enteral nutrition in the treatment of infants with "travelers' diarrhea" and severe malnutrition].

A prospective study of children aged 2-22 mos with traveller's diarrhea and severe malnutrition (weight loss greater than or equal to 10%; mean 17.8%) treated with a standardized progressive semi-elemental drip feeding (Alfaré and Dextrine-maltose) after rehydration was undertaken. In 18 children, this therapy was successful and duration of the hospital stay was 15.7 days. In 6 other children, relapse was treated with the same protocol with success and duration of the hospital stay was 29 days. Total parenteral nutrition was unnecessary. Evolution of serum prealbumin and anthropometric parameters was good.

Anthropometry↗
PubMedDate not supplied

[Visual seizures and benign epilepsy in children with paroxysmal occipital discharges].

The study of ocular symptoms during seizures and of electroencephalograms between seizures in 244 children affected by partial epilepsy has allowed the isolation of nine cases of benign epilepsy with occipital paroxysms. Ocular symptoms as well as other accompanying symptomatology are detailed. The diagnostic procedure should address secondary occipital epilepsy and migraine with ocular symptoms.

Adolescent↗