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Biomedical subjects

S Garrod

Publications and source records attributed to S Garrod.

5 recordsLinked to original sources
PubMed1992

Irradiation of the bovine mitochondrial F1-ATPase previously inactivated with 5'-p-fluorosulfonylbenzoyl-8-azido-[3H]adenosine cross-links His-beta 427 to Tyr-beta 345 within the same beta subunit.

The bovine heart mitochondrial F1-ATPase (MF1) is inactivated by 5'-p'-fluorosulfonylbenzoyl-8-azidoadenosine (8-N3-FSBA) with an apparent Kd of 0.47 mM at pH 8.0 and 23 degrees C in the absence of light. Irradiation of dark-inactivated enzyme with long-wavelength UV light produced cross-linked dimers and, to a lesser extent, trimers made up of alpha and beta subunits. Two major radioactive peptides were resolved by high-performance liquid chromatography from tryptic digests of MF1 which had been inactivated with 8-N3-FSB[3H]A at pH 8.0 in the dark. Sequence analysis revealed that one contained Tyr-beta 368 and the other contained His-beta 427 which were labeled in the ratio of 18:15. Sequence analysis of radioactive tryptic peptides isolated from digests of irradiated MF1 derivatized with 8-N3-FSB[3H]A showed that photolysis induced cross-linking of His-427 to Tyr-345 within the same beta subunit in high yield. When MF1 derivatized with 8-N3-FSB[3H]A was irradiated in the presence of beta-mercaptoethanol, alpha-beta cross-links were eliminated, whereas those between His-beta 427 and Tyr-beta 345 were unaffected. Analysis of radioactive peptides in tryptic digests of MF1 derivatized with 8-N3-FSB[3H]A and then irradiated in the presence or absence of beta-mercaptoethanol showed that the nitrene generated from reagent attached to Tyr-beta 368 participates in formation of alpha-beta cross-links in the absence of beta-mercaptoethanol. Therefore, the nitrene generated from reagent tethered to His-beta 427 is shielded from solvent and reacts with the side chain of Tyr-beta 345. In contrast, the nitrene generated from reagent attached to Tyr-beta 368 is exposed to solvent, but in the absence of scavengers reacts with side chains present in the alpha subunit. Irradiation of MF1, partially inactivated with 8-N3-FSBA, led to loss of residual ATPase activity without affecting residual ITPase activity. The amount of photoinactivation was greater when partial dark inactivation was performed at pH 6.9, where modification of His-beta 427 predominates, than when performed at pH 8.0, where modification of Tyr-beta 368 predominates. This suggests that cross-linking of His-beta 427 to Tyr-beta 345, and not cross-linking of alpha and beta subunits, is responsible for the augmented inactivation induced by irradiation.

Adenosine↗
PubMed1992

Discourse influences during parsing are delayed.

Subjects read sentences containing either a syntactically ambiguous prepositional phrase attachment or a syntactically ambiguous reduced relative clause. The sentences were embedded in passages of text that were consistent with either the minimal or non-minimal attachment reading. In addition, a discourse factor (i.e., whether or not the target sentence was in the discourse focus) was varied. Subjects' eye movements were recorded as they read the passages of text. Our primary finding was that subjects were garden-pathed even when there was biasing context. However, when the target sentence was in the discourse focus, subjects were able to recover more readily from their initial erroneous parse of the sentence. The data thus support models of sentence parsing that postulate that the parsing of a sentence is based upon structurally based principles and the influence of semantic or pragmatic information makes itself felt only after the initial parsing decision has been made.

Adult↗
PubMed1991

Chromogranin B: isolation from pheochromocytoma, N-terminal sequence, tissue distribution and secretory vesicle processing.

The chromogranins/secretogranins are a family of neuroendocrine vesicle secretory proteins. Immunohistology and immunoblotting have suggested that a major soluble protein in human chromaffin granules may be chromogranin B (CgB). We purified from pheochromocytoma chromaffin granules an SDS-PAGE 110-120 kDa protein whose N-terminal sequence matched that previously deduced from a human CgB cDNA. An antibody directed against a synthetic human CgB N-terminal region specifically recognized the CgB N-terminus, though not the chromogranin A (CgA) N-terminus or the CgB C-terminus on immunoblots. An antiserum directed against CgB's C-terminus also visualized CgB but not CgA. By immunoblotting, CgB was a quantitatively major protein in human pheochromocytoma chromaffin granules, but a relatively minor in normal bovine adrenal medullary chromaffin granules. In a variety of normal bovine neuroendocrine tissues, the relative abundance of CgB immunoreactivity on immunoblots was: adrenal medulla greater than anterior pituitary greater than pancreas greater than small intestine, hypothalamus. Immunoblotting of neuroendocrine tissues (or their hormone storage vesicle cores) with both anti N-terminal and anti C-terminal CgB antisera suggested bidirectional cleavage or processing of CgB; in the anterior pituitary, a unique 40 kDa C-terminal fragment was observed. Bidirectional CgB cleavage was also suggested on immunoblots of chromaffin tissue from three species (human, bovine, rat). C-terminal processing of CgB was also confirmed by amino acid sequencing of SDS-PAGE-separated, polyvinylidene difluoride membrane-immobilized CgB fragments from pheochromocytoma chromaffin granules. Whether such fragments possess biological activity remains to be investigated.

Adrenal Gland Neoplasms↗
PubMed1990

Elaborative inferencing as an active or passive process.

O'Brien, Shank, Myers, and Rayner (1988) reported that readers generated elaborative inferences only when a text contained characteristics that virtually eliminated the possibility of an inference being disconfirmed. We reanalyzed the data of O'Brien et al. (1988) and also conducted an experiment in which we varied (a) whether or not there was an anaphoric relation between a target word and its prior mention in the text and (b) the explicitness of the prior mention. Two refinements to O'Brien et al.'s conclusions are offered. First, the two text characteristics they manipulated (a strong biasing context or a demand sentence) may have produced different types of elaborative inferencing. We argue that a biasing context results in a passive form of elaborative inferencing, involving setting up a context of interpretation, whereas the presence of a demand sentence invites the reader to actively predict a subsequent expression. Second, clear evidence for either type of inference will be apparent only with truly anaphoric materials.

Attention↗