A rapid method for sectioning whole mature coniferous seeds for histological and histochemical study.
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Microscopy↗
Biomedical subjects
S H Farris
Publications and source records attributed to S H Farris.
Use of pectinase to dissociate plant nuclei for squash preparations: effect of hydration procedures.
Scale tissues taken from cones of pendent Douglas-fir Pseudotsuga menziesii (Mirb.) Franco were treated with pectinase to dissociate nuclei for Feulgen staining. Hydration of fixed tissues with water or a graded series of ethyl alcohol before pectinase treatment resulted in distention and faint staining of nuclei and inadequate disintegration of cell walls. These problems were overcome by adopting citrate buffer hydration. Analysis of digitized scans of nuclei provided information about the distribution of the stained material in density classes and nuclear size which is usually unavailable through the conventional double wavelength DNA measuring method.
Buffers↗
Image enhancement for light microscopy.
Digitized light microscope images of low contrast histological samples were processed through image enhancement computer techniques. Original digital data and their square root transformations were linearly expanded to produce a wider range of brightness levels in the enhanced images. These treatments resulted in significantly improved images compared to those obtained by direct photomicrography.
Animals↗
Enhancement of acid phosphatase detection by viewing stained insect muscle with polarized light.
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Acid Phosphatase↗