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Biomedical subjects

S H Jin

Publications and source records attributed to S H Jin.

24 records · Page 2Linked to original sources
PubMed1990

[Fuzzy cluster for analysis of the relationship between the structure of cephalosporins and immune cross-reaction].

Six parameters (molecular negentropy, acidic group number, basic group number, proton donor group number, proton acceptor group number, and a ratio of C atomic group number to total atomic group number) for characterizing the structure of an antibody combining site in a R1 chain of cephalosporins were selected. Although 12 parameters characterized the site A and site B in a R1 chain were used in fuzzy cluster, Fischer weighting ratio (Fi) indicated that only 5 parameters, 4 of them characterized the structure of site A, play an important part in the cluster. Therefore it was speculated that the site A was the major combining site in the antigen-antibody interaction. According to the similarity of the R1 chains, cephalosporins could be clustered into 4 groups among which less cross-reaction took place. Using the "relative Hamming distance" of the R1 chains for description of their similarity, we found that the intensity of the cross-reaction assayed by immune tests had a close correlation with the "relative Hamming distance", so the distance was used for prediction of the intensity of the cross-reaction of cephalosporins.

Binding Sites, Antibody↗
PubMed1989

[Immediate type anaphylaxis of streptomycin allergy elicited by streptomycin polymers].

It has been known that streptomycin (SM) can cause some immediate type anaphylaxis even anaphylactic shock in clinical therapy. The characteristic of the substance that elicits the allergic reaction has not been reported. Using gel filtration and high performance gel permeation chromatography (HPGPC), we have found that some impurities of high molecular weights (HMW) were formed by heating acidic SM solution. The HMW impurities had a colour reaction with citric acid-acetic anhydride reagent and elicited passive cutaneous anaphylaxis (PCA) on guinea pigs sensitized with rabbit anti-SM-BSA serum and general anaphylaxis in guinea pigs immunized by SM-BSA. It is suggested that the impurities, SM polymers (poly-SM) related to some reactions on amino groups of SM, are the allergens of SM allergy.

Animals↗
PubMed1989

[Electrophysiological studies of acute anoxic hypoxia].

This study was designed to analyse the effects of acute anoxic hypoxia on brainstem auditory evoked potentials (BAEPs) and electroencephalogram (EEG) and identify their sensitivities of response. After ether anesthesia, endotracheal intubation was performed on 20 adult Long-Evans rats. Relaxin was injected to immobilize the rats and a rodent respirator was provided for artificial ventilation. Acute anoxic hypoxia was induced by apnea with abrupt cessation of the respirator. The BAEPs and EEG were monitored and analyzed continuously before, during and after hypoxia. Experimental hypoxia was induced initially for 3 minutes and, after recovery, for another 5 minutes. Elapsed time was recorded as the waves altered or became flat. Characteristic wave changes were observed, which included delta bursts, epileptic discharges and alpha coma activity in the EEG; and decreased amplitude or delayed latency in the BAEPs. In all experiments, EEG flattening (71.80 +/- 7.80 sec) significantly preceded the flattening of the BAEPs (146.90 +/- 7.00 sec; Wilcoxon signed rank test, p less than 0.01). However, recovery after reoxygenation was more rapid in the BAEPs than in the EEG (162.80 +/- 18.90, 208.20 +/- 17.90 sec, Wilcoxon signed rank test, p less than 0.05). BAEP loss began with the later waves. In the EEG, during the recovery period epileptogenic foci (45%), delta bursting (45%) or alpha coma activity (40%) appeared. The isoelectric EEG and BAEPs were reversible in 12 of the 18 rats after 3-minutes of apnea and immediately followed by reoxygenation. The results of this study indicate the functional vulnerability of the various structures of the nervous system in regard to hypoxia.

Acute Disease↗
PubMed1989

[Erythrocyte deformability in experimental state and diseased RBC].

By Reid's functional test of erythrocyte deformability, the filtration time of 1 ml 5% erythrocyte suspension through a 5 micron nucleopore membrane was measured from 18 normal people and 19 patients with thalassemia. In the control group, the filtration time at 37.0 degrees C and pH 7.4 was 3.26 +/- 0.38 sec. Erythrocyte deformability was constant while blood temperature stayed between 35.0 degrees C and 42.0 degrees C. However the erythrocyte filtration time prolonged as the blood became alkaline or incubated for more than 2 hours. The filtration time of blood was 4.56 +/- 1.02 sec for thalassemia patients, significantly different (p less than 0.01) as comparing to the normal control group. Poor deformability in diseased blood could be explained by membrane defect. Erythrocyte deformability is a good rheological indicator if the physiological conditions of blood is controlled well.

Erythrocyte Deformability↗
PubMed1986

[Interaction of human embryo cells derived from different tissues with lymphoblastoid cell lines].

Cells from skin and muscle of two month human embryo and from nasopharynx, tonsil, lung and kidney of four month human embryo were cocultivated with lymphoblastoid cells--B95-8 and Raji. It is found that all the epithelial cells, such as the focal growth of epithelioid cells from the tonsil and nasopharynx as well as kidney cells from the human embryo can not adhere to any kind of lymphoblastoid cells; but fibroblasts from muscle and skin of two month embryo or from nasopharynx, tonsil and lung of four month embryo have the ability of sticking to lymphoblastoid cells. However, only the fibroblastoid cells from the nasopharynx of human embryo have the ability to stick and to fuse with lymphoblastoid cells--B95-8 in cocultivation and to activate the EBV production. The mechanism of this phenomenon and its biological functions will be studied in detail.

Cell Adhesion↗