Biomedical subjects

S H Kwa

Publications and source records attributed to S H Kwa.

3 recordsLinked to original sources

PubMed1995

Contraction characteristics and myosin heavy chain composition of rabbit masseter motor units.

1. We studied isometric twitch peak force (TPF) and twitch contraction time (TCT) of 249 motor units of the masseter muscle in 41 rabbits after extracellular electrical stimulation of single trigeminal motoneurons in the brain stem. In 41 of these units we determined the amount of tension decrease during a partially fused tetanus (sag) and the ratio between peak tetanic force after 2 min of intermittent tetanic stimulation and initial tension (fatigue index). Muscle fibers of 24 motor units were identified by the glycogen depletion method and characterized in serial sections with monoclonal antibodies against type IID, IIA, "cardiac" alpha, and I isoforms of myosin heavy chain (MHC). 2. The motor units had TCTs ranging from 13 to 32 ms. The majority of the units showed forces < 35 mN. The TPFs were larger and varied more for motor units with short and intermediate TCTs than for units with long TCTs. There is a small but statistically significant negative correlation between the motor unit TPF and the TCT. 3. All units exhibited "sag" and, with the exeption of one, had fatigue indexes > 0.75. The studied rabbit masseter motor units can therefore be classified as fast, fatigue-resistant, except for one that belonged to the FF (fast, fatigable) category. No slow units were represented in the sample pool. Significant correlations were not found either between TCT and the amount of sag or between TCT and the fatigue index. 4. Immunohistochemical analysis showed that the FF unit had fibers containing only IID-MHC. Five other units were found with a single MHC--three with IIA-MHC and two with alpha-MHC. In three other units all fibers showed one combination of two MHCs (1 IIA/IID, 1 IIA/alpha, and 1 alpha/I). The remaining 15 units contained two MHCs spread unevenly over the constituting fibers. Large variations in myosin composition of fibers within one motor unit cast doubts on the presumed dominant neuronal influence on myosin expression in the adult animal. 5. We found a close, statistically significant correlation between the TCT and the estimated MHC content of the units: the TCT was 13 ms for the IID unit, 18 ms for the pure IIA units, and 28 ms for the pure alpha units. Units with two MHCs had intermediate TCTs; units with alpha/I-MHC mixtures had TCTs of 29-30 ms. No pure MHC-I units were identified.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗

PubMed1995

Function-dependent anatomical parameters of rabbit masseter motor units.

Rabbit masseter motor units (22) were studied by stimulation of trigeminal motoneurons. We tested the hypotheses that masseter motor units facilitate fine motor control by concentrating fibers in small areas and that the distribution of motor unit fibers depends on the fiber type. The twitch contraction time and the isometric tetanic force were registered. The motor unit fibers were depleted of their glycogen by prolonged stimulation. Serial sections of the entire muscle were stained with the periodic acid Schiff (PAS) and monoclonal antibody stains. The muscle fibers of the motor unit were mapped and identified by four myosin heavy-chain (MHC) isoforms: I, IIA, IID, and cardiac-alpha. In the PAS-stained sections, anatomical parameters of the motor units, affecting the force output, were analyzed: the innervation ratio (IR), motor unit territory area (TA), and relative (R-DENS) and absolute (A-DENS) motor unit fiber densities. The fiber cross-sectional area (F-CSA) was measured for each MHC fiber type. The F-CSA sum of all motor unit fibers, the physiological cross-sectional area (P-CSA), was calculated. The IR ranged between 77 and 720 fibers (mean, 267). The mean TA was 8.71 mm2 (range, 4.45 to 19.58). The mean R-DENS was 10 fibers per 100; the A-DENS was 31 fibers per mm2. Linear correlations were found between the IR and the R-DENS and between the tetanic force and the IR. The F-CSAs showed a stepwise increase in value from type I- to IID-MHC fibers. The mean P-CSA was 0.90 mm2 (range, 0.09 to 2.97). A high linear correlation was noted between the P-CSA and the tetanic force. In conclusion, increase of motor unit size expressed in higher fiber counts and forces is accomplished by increase of the fiber density. Thus, forces can be exerted selectively in restricted regions of the masseter muscle. Differences in fiber orientation due to complex muscle pinnation emphasize the possibility of an accurate muscle performance.

Analysis of Variance↗

PubMed1993

Motor unit territories and fiber types in rabbit masseter muscle.

The myosin heavy chain (MHC) content and spatial distribution of the fibers of 11 motor units (MUs) of the rabbit masseter muscle were determined. The fibers of single MUs were visualized in whole-muscle serial sections by a negative periodic acid/Schiff reaction for glycogen after they had been depleted of glycogen by extracellular stimulation of their motoneuron in the trigeminal motor nucleus. The MHC isoforms present in the fibers were characterized by monoclonal antibodies. Individual fibers appeared to contain from one to three MHC isoforms. In six cases, all fibers of a motor unit had an identical MHC content; in five cases, different fiber types were found in a single unit. The fiber number per MU varied between 40 and 424, the territory size between 1.1 and 11.0 mm2 (of a total muscle cross-section of 200 mm2), and fiber density between 6 and 17 MU fibers per 100 muscle fibers. In the multipennate masseter, the fibers were usually restricted to a single anatomical compartment. In comparison with leg muscles, the fibers of the masseter motor units, although similar in number, were restricted to relatively smaller subvolumes of the muscle and thus reached higher densities in their territories. The small territories are the anatomical substrate for the observed heterogeneity of motor behavior. Since the different anatomical compartments of the masseter differ with respect to their biomechanical capabilities, this makes this muscle multifunctional in the exertion of complex motor tasks.

Animals↗