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Biomedical subjects

S H Mitchell

Publications and source records attributed to S H Mitchell.

33 records · Page 2Linked to original sources
PubMed1995

Susceptibilities of Chlamydia trachomatis isolates causing uncomplicated female genital tract infections and pelvic inflammatory disease.

The in vitro susceptibilities of 45 recent clinical isolates of Chlamydia trachomatis obtained from women with asymptomatic genital tract infection, mucopurulent cervicitis, or pelvic inflammatory disease to doxycycline, azithromycin, ofloxacin, and clindamycin were determined. In addition, susceptibilities of 12 isolates to amoxicillin and trimethoprim-sulfamethoxazole were also determined. Isolates also were serotyped with a panel of monoclonal antibodies specific for chlamydial major outer membrane protein; 24 of 45 (53%) belonged to serovars Ia and E. For all isolates, the MIC range of doxycycline was 0.008 to 0.06 micrograms/ml, for trimethoprim-sulfamethoxazole it was 0.03 to 0.25 micrograms/ml, for azithromycin it was 0.125 to 2.0 micrograms/ml, for ofloxacin it was 0.5 to 1.0 micrograms/ml, for clindamycin it was 0.25 to 2.0 micrograms/ml, and for amoxicillin it was 0.25 to 4.0 microgram/ml. The ranges of minimum chlamydiacidal concentrations were generally 1 to 4 dilutions above the MICs of most agents, with a rank order similar to those of the MICs. Comparing the minimum chlamydiacidal concentrations for 90% of isolates tested, isolates causing asymptomatic infection belonged to a greater variety of serovars and were relatively more susceptible to doxycycline and azithromycin than isolates causing mucopurulent cervicitis or pelvic inflammatory disease; these differences in susceptibility were not detected among the other study agents. These data indicate that additional studies are needed to better define the apparent association of certain chlamydial serovars with the clinical severity of disease and the in vitro susceptibilities to certain antimicrobial agents.

Anti-Bacterial Agents↗
PubMed1992

Tissue concentrations of organochlorine compounds in common seals from the coast of Northern Ireland.

An epizootic of morbillivirus infection killed several hundred common seals (Phoca vitulina) along the coast of Northern Ireland in 1988. Many dead and moribund seals were submitted to the Department of Agriculture for Northern Ireland, Veterinary Research Laboratories for necropsy. Samples of blubber, liver and kidney were collected from these animals (n = 55) for analysis for a wide range of organochlorine pesticides and also total polychlorinated biphenyls (PCBs). The organochlorines found in highest concentrations in blubber were PCBs which were detected in all samples (mean, 26 micrograms g-1 wet wt). sigma DDT (mean, 2.63 micrograms g-1 wet wt) and chlordane (mean, 0.22 micrograms g-1 wet wt) were also present in all samples. Heptachlor including heptachlor epoxide (mean, 0.13 micrograms g-1 wet wt) was present in over 93% of samples. Seventy-four percent of samples contained alpha HCH (mean, 0.035 micrograms g-1 wet wt), 50% contained beta HCH (mean, 0.026 micrograms g-1 wet wt) while gamma HCH (mean, 0.012 micrograms g-1 wet wt) was detected in 46% of samples. The organochlorine concentrations found in this study are at the lower end of the range previously reported for European common seals.

Adipose Tissue↗
PubMed1991

Energetic and motor responses to increasing force requirements.

The effects of increasing work (force) requirements on energy expenditure and response topography were examined in 7 rats pressing a beam to earn food. For the 1st 16 days, the force requirement was 5.52 x 10(-2) N (5.625 g). This increased by 4.91 x 10(-2) N (5 g) every 7th session until Ss had experienced 10 upward shifts. Following the 54.57 x 10(-2) N (55.625 g) condition, the original criterion was reinstated. During the augmented phase. Ss maintained stable reinforcement rates across conditions by increasing the peak force of beam pressing. These higher forces, occurring within 20 reinforcements of changing the force criterion, were produced primarily by increases in the rate of change of force (delta F/delta T). Also, while the rate of work performed on the beam increased, the overall energy expenditure fell. In contrast to these rapid adjustments, reinstating the original 5.52 x 10(-2) N (5.625 g) criterion resulted in only gradual alterations in motor performance.

Animals↗
PubMed1990

Transplant information management system: a center-oriented approach to transplant data management.

TIMY is the ideal management system for novice data-entry personnel as well as for the research staff, nurses, and physicians at our facility. Access to data through the use of menus and security systems makes TIMY a user-friendly database. It has excellent data export capabilities so that more complex data analysis can be performed using dedicated software such as SPSS/PC, BMDP, or SAS. This provides great flexibility as well as the power needed for the study of complex data sets.

Database Management Systems↗
PubMed1987

Immunocytochemical demonstration of 5-hydroxytryptamine (serotonin) in the nervous system of the liver fluke, Fasciola hepatica (Trematoda, Digenea).

The localisation and distribution of 5-hydroxytryptamine (5-HT or serotonin) in the nervous system of Fasciola hepatica has been determined by an indirect immunofluorescence technique. Cell bodies and nerve fibres immunoreactive to 5-HT are present in the anterior ganglia, and the longitudinal nerve cords and their commissures in the central nervous system. In the peripheral nervous system, similar immunoreactivity occurs in the nerve plexuses supplying the sub-tegumental muscle layers and the muscular lining of various reproductive ducts, including the ootype, uterus and cirrus pouch. The significance of these results in the light of previous studies on the role of 5-HT in F. hepatica is discussed.

Animals↗
PubMed1982

Isolation of mycoplasmas and bacteria from the blood of postpartum women.

Mycoplasmas and/or bacteria were recovered from the blood cultures of 26 (20.8%) of 125 febrile postpartum women. Genital mycoplasmas were recovered from 16 (12.8%) of the 125 febrile women and from none of 60 afebrile postpartum women (p less than 0.005). The presence of mycoplasmaemia was associated with a young age, primigravidity, and nulliparity. The isolation of organisms from the blood was also associated with fever during labor, internal monitor use, and a cesarean delivery.

Adult↗
PubMed1977

Enzyme-linked immunosorbent assay for chlamydial antibodies.

An enzyme-linked immunosorbent assay (ELISA) detected chlamydial antibodies in human sera. The assay antigen produced in cell cultures infected with Chlamydia psittaci was Formalin-fixed to microplates. Single convalescent-phase sera positive for chlamydial antibodies by a complement-fixation test were positive at even higher dilutions by ELISA. Paired sera with diagnostic rises in complement-fixing antibody showed seroconversion by ELISA also. Control sera from persons with no history of chlamydial infection were negative by both tests. Sera from patients with psittacosis or lymphogranuloma venereum were ELISA positive, indicating that the assay with the antigen used in this study is genus specific rather than species specific.

Antibodies, Bacterial↗
PubMed1976

Accelerated detection of lymphocytic choriomeningitis virus in diagnostic specimens.

Lymphocytic choriomeningitis virus could be demonstrated earlier in mice inoculated with clinical specimens if the mice were sacrificed before they appeared sick for examination by the fluorescent antibody technique. In the procedure, the optimal route for inoculation was intracerebral and the best organ tested for staining was the brain.

Animals↗
PubMed1976

A new technic for obtaining blood from mice.

A practical technic was described for collecting serial blood samples from mice. Blood was obtained from a tail incision made immediately after the mice had been exposed to an ambient temperature of 45 degrees C. Individual samples sufficient for most tests were readily obtained without significant hemolysis. The technic was rapid, reliable, humane, and required no special skill.

Animals↗