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Biomedical subjects

S H Opdal

Publications and source records attributed to S H Opdal.

9 recordsLinked to original sources
PubMed1999

The complement component C4 in sudden infant death.

UNLABELLED: The aim of the present study was to compare partial deletions of the complement C4 gene in victims of totally unexplained sudden infant death (SID) (n = 89) and borderline SID (n = 15) with and without slight infections prior to death, in cases of infectious death (n = 19), and in living infants with and without infections (n = 84). The SID and borderline SID groups were pooled. In this total SID group slight infections prior to death was associated with deletion of either the C4A or the C4B gene (P = 0.033), and the SID victims with such infections had a higher deletion frequency than the controls (P = 0.039). There were no differences between the living infants with and without upper airway infections. CONCLUSION: The present study confirms that partial deletions of the C4 gene in combination with slight upper airway infections may be a risk factor in sudden infant death.

Child, Preschool↗
PubMed1999

Mitochondrial DNA point mutations detected in four cases of sudden infant death syndrome.

The aim of this study was to investigate the tRNA(Leu(UUR)) gene and the first part of the ND1 gene in mitochondrial DNA (mtDNA) in cases of sudden infant death syndrome (SIDS). A total of 158 cases of SIDS and 97 controls were included in the study, and the base pairs in the range 3230-3330 were investigated using polymerase chain reaction (PCR) and temporal temperature gradient electrophoresis (TTGE). If a band shift was detected by TTGE, the area investigated and the D-loop was sequenced. Three different point mutations (T3290C, T3308C and T3308G) were detected in four of the SIDS cases, while none of the controls were mutated. We also found a high D-loop substitution rate in these four cases. The findings indicate that mtDNA mutations may play a role in some cases of SIDS.

DNA, Mitochondrial↗
PubMed1998

SIDS--changes in the epidemiological pattern in eastern Norway 1984-1996.

The objective was to analyse differences in the epidemiological pattern of sudden death in infancy during two time periods--the Sudden Infant Death Syndrome (SIDS) 'epidemic': 1984-1989, and the period of rapid decline in the SIDS rate 1990-1996. Sex distribution, age, sleeping position, signs of infection, day of the week and place of death were registered and compared for the two time periods studied in all SIDS cases autopsied at the Institute of Forensic Medicine, Oslo. There were significantly more deaths in the age group under four months in the period 1984-89 than in the second period. Prone sleeping position, signs of infection, death outdoors and during the winter were more frequent during the first period than in the second. These features also were more frequent in the age group under four months than in the older babies during the first period. The shift in the epidemiological pattern after 1990, when the risk factor campaign was launched, indicates that prone sleeping position, cold climate, sleeping outdoors and infections seem to be risk factors that are particularly harmful to the youngest infants.

Humans↗
PubMed1998

Objective measurements of nicotine exposure in victims of sudden infant death syndrome and in other unexpected child deaths.

OBJECTIVE: Self-reported maternal smoking is associated with a dose-related-increase in the risk of sudden infant death syndrome (SIDS). The aim of this study was to measure objectively whether victims of SIDS are more exposed to tobacco smoke before death than infants who die unexpectedly of other causes. DESIGN: Continine levels in pericardial fluid were used as an indicator of exposure. Levels > 5 ng/mL indicated significant exposure, and levels > 20 ng/mL indicated heavy exposure. Samples were obtained from all sudden deaths in children < 7 years of age that occurred from 1990 through 1993 in southeastern Norway. Twenty four infants died of SIDS, 12 infants of infections, and 9 of accidents (median age 4.5, 5, and 35 months, respectively). RESULTS: Compared with the age-matched infectious deaths, a significantly higher proportion of victims of SIDS had been significantly (92% vs 67%) or heavily exposed (25% vs 0%) to nicotine, (P < .05). Median cotinine levels in infants with SIDS, 15.8 ng/mL, were significantly higher than in infants who had infectious deaths 7.1 ng/mL (P < .003) but were comparable to those of accident victims (12.9 ng/mL, not significant). CONCLUSIONS: Victims of SIDS are more often and more heavily exposed to tobacco smoke doses before death than are infants who have sudden infectious deaths. Accidental death in infancy and childhood is often associated with a significant exposure to nicotine.

Humans↗
PubMed1998

Increased number of substitutions in the D-loop of mitochondrial DNA in the sudden infant death syndrome.

The purpose of the present study was to investigate substitutions in the D-loop of mitochondrial DNA (mtDNA) in sudden infant death syndrome (SIDS) and controls, since several observations indicate the involvement of mtDNA mutations in SIDS. These include elevated levels of vitreous humour hypoxanthine in SIDS victims, familial clustering without mendelian traits, and observations of increased sleepiness and a lower activity score in infants who later succumbed to SIDS. Eighty-two cases of SIDS and 133 controls were investigated and the D-loop sequences were recorded in the base-pair range 16055-16500 in the mtDNA sequence. The sequencing was carried out using the Applied Biosystems Sequenase dye terminator method and a ABD373A sequencer. The recorded D-loop sequences were compared with the Cambridge sequence and differences were recorded as substitutions. The SIDS cases had a tendency towards a higher substitution rate in the D-loop than the controls (p = 0.088). This observation makes it interesting to search for deleterious mutations in other locations in the mtDNA.

DNA Mutational Analysis↗
PubMed1998

Hypoxanthine levels in vitreous humor: a study of influencing factors in sudden infant death syndrome.

Elevated hypoxanthine (Hx) levels in the vitreous humor of victims of the sudden infant death syndrome (SIDS) have been been claimed to indicate hypoxia before death. An important methodologic problem is the temperature-dependent Hx increase after death. Although most previous studies have corrected for expected postmortem Hx increase, the aim of the present study was to omit this problem by matching the subjects of the different groups studied for similar postmortem time. Thirteen cases of violent death, 11 cases of borderline SIDS, 22 cases of death from infectious disease, and 17 cases of death from heart/lung disease were compared with a 3-fold number of SIDS cases. To investigate the impact of environmental factors on the Hx levels of the SIDS cases, the following possible confounding factors were investigated: attempt to resuscitate, slight infections before death, sleeping position, and time of the day when found dead. To investigate whether the size of the eyeball would influence the Hx level, five cases of violent death in children were compared with 15 adults who died suddenly. The vitreous humor Hx level was significantly higher in SIDS victims than in cases of violent death (p < 0.01) and deaths due to heart/lung disease (p < 0.01), whereas no such difference was found either between SIDS and borderline SIDS or SIDS and infectious death. There were no differences between any of the subgroups of SIDS victims. The vitreous humor Hx level in small children and adults did not differ. The findings raise the question of possible similarities in death mechanism in SIDS and infectious death.

Age Factors↗
PubMed1994

Is partial deletion of the complement C4 genes associated with sudden infant death?

The two C4 loci C4A and C4B in 61 cases of sudden infant death (SID), 93 living controls and 7 cases of infectious death were studied. In the SID group 13.1% showed deletion of the C4A gene, while 2.5% of the cases showed deletion of the C4B gene. This was not significantly different from neither the controls nor the infectious death group. We were not able to confirm that deletion of the C4B gene is associated with SID. However, in the SID group deletion of either the C4A or the C4B gene was associated with signs of infections prior to death (P = 0.035). This observation may indicate that a proportion of SID victims are more vulnerable to infections than other infants.

Adult↗
PubMed1994

Vitreous humor hypoxanthine levels in SIDS and infectious death.

Hypoxanthine concentrations in vitreous humor were determined in 107 cases of sudden infant death syndrome (SIDS) and compared with levels in 4 cases of borderline SIDS, 26 cases of infectious death and 16 cases of sudden violent death. The hypoxanthine measurements were made using a high-performance liquid chromatography method. The hypoxanthine levels were significantly (p < 0.01) higher in SIDS than in violent deaths, while no significant difference was found between SIDS and infectious deaths. The present report demonstrates a similar distribution pattern of hypoxanthine levels in vitreous humor in SIDS and infectious death. We have previously described signs of immune stimulation both in peripheral organs and in the central nervous system in these conditions. This indicates that the death mechanism in SIDS has some similarities with infectious death.

Age Factors↗