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Biomedical subjects

S H Sherif

Publications and source records attributed to S H Sherif.

4 recordsLinked to original sources
PubMed1989

A scanning electron microscopic study of exfoliated malignant oral epithelial cells.

Investigators disagree regarding the value of exfoliative cytology in the diagnosis of oral cancer. The objective of the present study was to examine exfoliated oral epithelial cells from malignant lesions of the oral mucosa by means of Scanning electron microscope (S. E. M.) in order to determine whether SEM surface characteristics might be useful and specific to predict more accurately the nature of these lesions. Twenty cases of squamous cell carcinomas of non keratinized sites of the oral mucosa (Cheek and floor of the mouth) were selected for this study. Exfoliated cells were collected by scraping by means of modified Ayre's spatula and spread over a standard glass microscope slide for light microscope examination, while those for SEM were spread evenly on a plastic square (1 cm2) cut from a polyester sheet of Melinex "O" (Firket 1966). Altered tissue architectures and cell surface architectures were demonstrated in malignant oral lesions. Bizarre cell forms often showing an absence of close cell-cell contact relationships were seen. The observed cell surface patterns were apparently not related to the degree of keratinization when compared to normal surface patterns of corresponding areas of oral mucosa studied in a previous work. These changes appeared to some extent to be related to the clinical diagnosis and to the degree of epithelial atypia observed by the lesions. In conclusion the observations made in this study demonstrate that surface structural differences exist between exfoliated cells from malignant lesions of the oral mucosa and those from corresponding normal non keratinized sites and that the SEM might be of a diagnostic value.

Carcinoma, Squamous Cell↗
PubMed1987

Biologic evaluation of SPAD. I. A comparison between the tissue reactions to SPAD, Gelfoam, and silver amalgam.

The biologic properties of Traitement SPAD, a material suggested for filling root canals and the periapical region in cases of periapical involvement, were tested and compared with those of Gelfoam and silver amalgam. Empty polyethylene tubes were used as a negative control. The reactions of the subcutaneous tissues of rats to the test and to the control materials are reported. The initial tissue reaction to SPAD was very mild. However, this reaction increased with time to become moderate and persisted throughout the period of the study with no signs of absorption of the material. The inflammatory response to Gelfoam was initially greater. However, it decreased with time and the material was removed by the macrophages with subsequent reduction in the size of the surgical gap. The greatest inflammatory reaction was recorded after the use of silver amalgam, with the formation of capsules around the specimens, indicating that the material was extremely irritant before it had set. The longer the silver amalgam remained in situ, the milder the tissue reaction became.

Animals↗