Biomedical subjects
S Hashizume
Publications and source records attributed to S Hashizume.
Tissue dispersion, cell harvest and fluid suspension culture by the use of bacterial neutral protease.
Bacterial neutral protease of Bacillus polymyxa was found to disperse mammalian tissues and cells. Primary cell cultures were obtained from several tissues after treatments with 200 to 2,000 Kunitz unit per ml of this protease in either a phosphate buffer solution, a balanced salt solution or a tissue culture medium supplemented with serum. Monolayer cultures wither in their early passage levels or of established strains were harvested by a treatment with this protease, and proliferated again in monolayer after its removal. A growing culture of strain L-929 was kept in monodisperse suspension in the presence of this protease. In contrast to trypsin, this protease was found active in the presence of serum, stable during incubation and scarcely injured cells.
[Isolation of Mycoplasma from mammalian cell lines and its elimination by treatment with antibiotics (author's transl)].
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Preparation and laboratory tests of oil-adjuvant cholera vaccine.
In a search for cholera vaccines of improved efficacy, agar-grown strains of classical Vibrio cholerae were killed with formol and emulsified with Arlacel A in mineral oil; the final vibrio concentration was adjusted to 2 x 10(9) vibrios per adult dose (equal proportions of Inaba and Ogawa types).There was an unexplained discrepancy between determinations of the vibrio content, by cell counting and opacity measurements, and of the antigen content, by nitrogen measurements and the complement-fixation test.The potency of the vaccine, estimated by the mouse-protection test, was about 5 times that of the International Reference Preparations of Cholera Vaccine. A new potency test, the "maternal-immunity" test in infant rabbits, did not give quantitative results but suggested a somewhat higher potency for the vaccine.The protective effect 6 months after vaccination, as determined by agglutinin titres in the sera of volunteers, was still high, confirming the results of a field trial of the vaccine. However, local reactions (indurations) were observed in a considerable proportion of vaccinees in the field trial. Histopathological examination of tissue from the site of the reactions revealed them to be due to a combination of foreign-body reactions and local hypersensitivity.