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Biomedical subjects

S Hassam

Publications and source records attributed to S Hassam.

23 records · Page 2Linked to original sources
PubMed1990

Three cases of neonatal herpes simplex virus infection presenting as fulminant hepatitis.

We report three cases of neonatal herpes simplex virus (HSV) infection presenting as fulminant hepatitis. None of the patients had clear risk factors for HSV infection and they all died. Antiviral treatment for HSV is currently available but must be administered early in the course of the disease before irreversible liver tissue damage is present. Since the diagnosis may be difficult to establish, we wish to draw the attention of clinicians to the presentation of neonatal HSV infection and suggest that in such cases viral cultures, including culture of liver tissue, should be obtained early and antiviral treatment administered while awaiting the culture results.

Acute Disease↗
PubMed1990

In situ hybridization for the detection of human parvovirus B19 nucleic acid sequences in paraffin-embedded specimens.

Parvovirus infection of pregnant women leading to a transplacentar infection of the fetus may result in hydrops fetalis, and ultimately in intrauterine death of the fetus. In situ hybridization with a biotinylated as well as with a 35S-labeled probe for human parvovirus B19 was performed on formalin-fixed paraffin-embedded (FFPE) tissue from a fetus suffering from non-immunologic hydrops fetalis. Histology was suggestive of viral infection probably with human parvovirus. Parvovirus DNA could be detected and precisely localized mainly in the nuclei of erythroid precursors cells within fetal blood vessels of all organs examined. There was no detection of B19 nucleic acid in parenchymal cells of the placenta or the fetal organs, nor within maternal blood cells. These findings are in agreement with the well-known properties of animal parvoviruses to replicate exclusively in proliferating cells. Taking into consideration the problems in diagnosing human parvovirus infection by light microscopy, we conclude that in situ hybridization with an appropriate non-radioactive probe is a valuable, rapid and safe complementary detection method for the diagnosis and study of human parvovirus infections. The 35S-labeled probe is more sensitive than the biotinylated probe, but has the disadvantages of lower resolution of the signal, longer duration of the assay, the hazard of radioactivity and the shorter shelf-life of the probe.

Adult↗
PubMed1990

[Demonstration of the differential expression of IGF-II in various embryonal kidney tumors using slot-blot hybridization and in-situ hybridization].

IGF-II may play a decisive role in the development of Wilms tumors since an elevated expression of this important embryonal growth factor has been reported in the majority of nephroblastomas. In our series of 15 typical triphasic or blastemal predominant nephroblastomas slot-blot-hybridization revealed a marked increase of IFG-II-mRNA in the tumor tissue of 11 patients. Compared to normal kidney tissue IGF-II-expression was elevated up to 64 times. Apart from nephroblastoma a number of other embryonal renal tumors with either a much better or much worse prognosis was investigated. A moderately increased expression of IGF-II was noted in 2 congenital mesoblastic nephromas. IGF-II-mRNA was only slightly increased in a clear cell sarcoma of the kidney but was not elevated in 2 malignant rhabdoid tumors of the kidney. In-situ-hybridization allowed precise localization of the markedly increased IGF-II mRNA to blastemal cells. Differentiation to epithelial structures such as tubules or glomeruli or to stromal cells was associated with a marked loss in IGF-II expression.

Humans↗
PubMed1989

[Increased expression of IGF-2 in tumor tissue of nephroblastoma].

The incidence of nephroblastoma is increased in a number of syndromes with abnormal growth pattern. Elevated IGF 2-expression has been documented in various Wilms' tumors and a defect in the IGF 2 gene has been noted in 1 case. Southern blot analysis of genomic DNA of nephroblastomas in 5 additional patients after restriction enzyme digest with EcoRI, Pvu II, Pst I and Taq I did not reveal any defect in the IGF 2 gene. Slot blot analysis however showed marked overexpression of IGF 2-mRNA reaching up to more than 25 times the level expressed in unaffected kidney tissue adjacent to the tumor and in normal kidney tissue used as controls.

Blotting, Southern↗