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S I Gorodetskiĭ

Publications and source records attributed to S I Gorodetskiĭ.

At least 19 recordsLinked to original sources
PubMed1989

[The Bos taurus casein genes. Isolation and characterization of the kappa-casein gene].

A region spanning 25 kb of genomic DNA containing the kappa-casein gene, has been isolated from two genomic libraries in EMBL3 and EMBL4 phage vectors. Five phage clones containing kappa-casein gene have been found. Gene organisation has been determined using restriction mapping and a partial sequencing the 5' and 3' flanking regions. The kappa-casein gene includes 5 exons, the first of them coding for 64 nucleotides from the 5' untranslated mRNA zone. The gene is 12.5 kb long, which is almost 16 times longer than the corresponding mRNA. The first intron spans 2.5 kb, the second is the largest one and spans 5.5 kb. The 5' flanking region sequence has been analysed; it contains a TATA box from -30 to -25 bp, somewhat different from the canonic sequence, and a CAAT box at -80 bp.

Animals↗
PubMed1988

[Change in the level of casein mRNA in the mammary gland of rats and goats during lactation].

Cloned cDNAs for three distinct casein mRNAs (alpha s1-, beta- and chi) were used to determine the levels of individual mRNAs in total cell RNA preparations from both rat mammary glands in the course of natural lactation cycle and goat mammary glands at the most important lactation stages. Two maxima of active rat mRNA synthesis were shown to be closely associated with the particular developmental features of the progeny. A negative correlation between rat alpha s1-, and beta-casein mRNA levels was found. The length of goat casein mRNA was relatively increased at certain periods of the lactation cycle. The possible regulation mechanisms for the control of casein gene expression are discussed.

Animals↗
PubMed1988

[The casein genes of Bos taurus. II. Isolation and characteristics of the beta-casein gene].

The expression of the casein genes in mammary gland cells is regulated by peptide and steroid hormones. To study underlying regulatory mechanisms, the bovine beta-casein gene was isolated and characterized from lambda bacteriophage bovine DNA library. The beta-casein gene is 8.6 kb long and is 7.8 times longer than the mature casein mRNA coded for by 9 exons. The genomic clones incorporate additional 8.5 and 4.5 kb of the 5'- and 3'-flanking regions. The nucleotide sequences of 5' and 3' ends of the beta-casein gene are determined. Conserved sequences identical or homologous to potential sites of binding with the nuclear factor CTF/NF-1, glucocorticoid and progesterone receptors were identified. The regulatory region of the casein gene contains two different TATA signals flanking the duplication site in the promoter region.

Animals↗
PubMed1987

[Cloning and nucleotide sequence analysis of the cDNA of the immunoglobulin lambda light chain from the bovine udder].

A cDNA library was constructed from the mRNA of bovine mammary gland which contained Ig lambda producing plasmacytes. Overlapping clones encompassing the major portion of the coding sequence of the Ig lambda mRNA were isolated and sequenced. Predicted amino acid sequence shows a mature polypeptide of 217 residues in which V, J and C regions can be distinguished. The constant region has greatest homology with human Ig lambda mRNA constant region. The area of the V region between CDR3 and CDR2 has also great homology with the same area of human lambda chain.

Animals↗
PubMed1987

[Nucleotide sequence of the cDNA of kappa casein in cows].

Nucleotide sequence of the cloned DNA complementary to cow kappa-casein mRNA was determined. The complete sequence is composed of 854 bases and includes 60 bases of 5'-noncoding region, 570 bases of the coding region and 209 bases of 3'-noncoding region without poly(A). The cloned sequence codes for kappa-casein, the genetic variant B2. Several restriction sites were defined that permitted the identification of genetic variants and size polymorphism of restriction fragments in the structural region of the gene. Upon the comparative analysis of kappa-casein mRNAs of cow and rat, it was shown that, apart from functionally important regions in the coding sequence, high homology is characteristic of the 5'- and 3'-noncoding regions.

Amino Acid Sequence↗
PubMed1986

[cDNA of cattle alpha S1-casein: cloning and nucleotide sequence].

The nucleotide sequence of recombinant plasmids representing a full-size cDNA of cow alpha s1-casein was investigated. The corresponding mRNA consists of 1133 nucleotides except for poly(A) and includes 642 nucleotides of the coding region, 63 nucleotides of 5'- and 428 nucleotides of the 3'-noncoding regions. A comparative analysis of nucleotide sequences of cow alpha s1-casein and guinea pig B-casein showed that the homology in the 5'-nontranslatable region is 90.5%, that of a precasein single peptide is 82.22%, while that of the major polypeptide in the coding region is 64% without taking into account the blank spaces. The homology is higher in the 3'-noncoding region than in the coding region and makes up to 72%. The data obtained testify to the high degree of conservatism of sequences in casein mRNA noncoding regions as well as to functional and regulatory role of these sequences in gene expression of caseins.

Amino Acid Sequence↗
PubMed1986

[Stimulation and inhibition of the growth of mice carrying the human growth hormone gene].

The human growth hormone gene, containing mouse metallothionein gene promotor, was injected into the male pronuclei of fertilized mouse ova. The progeny of transgenic mice included animals with both accelerated and inhibited growth. Radioimmunochemical analysis has revealed human growth hormone synthesis in both groups of transgenic mice. The molecular weight of the hormone synthesized in liver cells was 25,000 daltons. A possible mechanisms of foreign hormone effect on the growth of transgenic mice is discussed.

Animals↗
PubMed1985

[mRNA localization and content in bovine kininogen].

The bradykinin (BK) gene chemically synthesized and cloned into pBR 322 was used for the study of tissue localization and quantitation of mRNA coding for the BK precursor, kininogen. Poly(A+)-mRNAs from bovine liver, spleen, kidney, mammary gland and pancreas were used for dot-hybridization to [32P]DNA of the BK gene or to the plasmid-containing BK gene. The experimental results demonstrate that [32P]DNA of BK is hybridized only to the liver poly(A+)-RNA, which proves the liver to be the main kininogen mRNA-producing tissue. In other tissues, the kininogen mRNA synthesis is either altogether absent or its level is two orders of magnitude less as compared to the liver. Several approaches for the quantitation of the kininogen mRNA were developed. The amount of this mRNA was shown to be about 0.6% of the total cellular poly(A+)-RNA. Poly(A+)-RNA which is bound to BK DNA-cellulose and is enriched with BK-coding sequences was used for the study of the hybridization kinetics and translation in a cell-free system from rabbit reticulocytes. The polypeptides synthesized contain BK as was shown by the use of a bio-test in rat uterus.

Animals↗
PubMed1985

[Synthesis and cloning of DNA complementary to mRNA of the bovine mammary gland].

Poly(A+)mRNA from bovine mammary glands was used to synthesize double-stranded cDNAs that were subsequently inserted into the plasmid vector pBR322 at the Pst1 site by means of oligo(dG)-oligo(dC) tailing. After transfection of Escherichia coli JC5183, recombinant plasmid library containing 5400 clones was screened by serial rounds of colony hybridization in situ to total [23P] poly(A+)mRNA and electrophoretically homogenious [32P]16SmRNA of mammary glands. Then hybrid selection of mRNA and subsequent in vitro translation of selected mRNAs were performed. In this manner, recombinant clones coding for alpha S1- beta-, kappa-casein were identified. cDNA clones range in size from 35% for beta-casein, 65% for alpha S1-casein to about 95% for kappa-casein, in comparison with their respective mRNAs.

Animals↗
PubMed1985

[Restriction analysis of cDNA for cow alpha s1-, beta- and kappa-caseins].

By means of in situ hybridization to cloned cDNA fragments coding for cow alpha s1-, beta- and kappa-caseins, screening of the library of clones containing the cDNA complementary to mRNA of lactating cow mammary gland was carried out. The clones containing the sequences of alpha s1-, beta- and kappa-casein cDNAs were shown to constitute 4.0, 3.2 and 0.7% of all the colonies, respectively. The analysis of the data on cross-hybridization points to the absence of extensive regions of homology between the above-mentioned cDNAs. The restriction analysis of cDNAs of the selected clones was carried out and the restriction maps of cDNAs of these three caseins were constructed. The restriction analysis data and determination of the nucleotide sequence of 5'-termini of the studied cDNAs indicated that the cloned sequences were the full-length mRNA copies of alpha s1-, beta- and kappa-caseins. The data obtained on restriction analysis are utilized in mapping the corresponding natural genes of cow caseins.

Animals↗