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Biomedical subjects

S Inooka

Publications and source records attributed to S Inooka.

10 recordsLinked to original sources
PubMed1996

Sphingosine transfer in cell-to-cell interaction.

We have investigated possible intercellular transfer of Sph between heterogeneous and homogeneous cells, utilizing different abilities on conversion of Sph into GlcCer. Murine IL-2-dependent T lymphocyte CTLL cells metabolize exogenously added Sph to GlcCer through Cer within 30 min, whereas fibroblast BALB/C A31 cells required more than 2 h. Thus, CTLL cells exhibiting the active conversion were used as recipient cells, whereas A31 cells where the conversion was slow and fumonisin B1 (inhibition of Cer biosynthesis)-treated CTLL cells being donor cells. To test intercellular transfer of Sph, donor cells incorporated radioactive Sph were coincubated with CTLL cells and GlcCer was examined. The present experiments described the possible transfer of Sph between heterogeneous (from A31 cells to CTLL cells) and homogeneous cells (from fumonisin B1-treated CTLL cells to CTLL cells).

Animals↗
PubMed1996

Use of fumonisin B1 to test the intercellular transfer of sphingosine.

Sphingosine, a core unit of sphingolipids, has been shown to mediate intracellular signaling events. It is conceivable that sphingosine could act as an intracellular messenger as well as an intracellular modulator, if sphingosine were to be transferred intercellularly. Murine interleukin-2 (IL-2) dependent T-lymphocyte CTLL cells, murine fibroblasts Swiss 3R3 cells, and murine fibroblast BALB/C A31 cells metabolize exogenously added sphingosine to ceramide. Fumonisin B2, a mycotoxin produced by Fusarium moniliforme, blocks the conversion of sphingosine to ceramide. In the study described here, CTLL cells exhibiting the conversion of sphingosine to ceramide were used as recipient cells, whereas fumonisin B1-treated cells (A31 cells, Swiss 3T3 cells and CTLL cells), in which the conversion was blocked were used as donor cells. To demonstrate intercellular transfer of sphingosine through the conversion pathway of sphingosine to ceramide, fumonisin B1-treated donor cells incorporating radioactive sphingosine were co-incubated with CTLL cells and the ceramide response was examined. These experiments demonstrated that it is possible to prove the intercellular transfer of sphingosine by using different activities in the cellular conversion pathway of sphingosine to ceramide.

3T3 Cells↗
PubMed1986

Production of cytotoxin to a tumor cell in co-cultures of macrophage cell line with bovine leukemia virus producing cell line.

J 774 cells (macrophage cell line) were co-cultured with fetal lamb kidney cells which permanently produce Bovine leukemia virus (BLV-FLK cells) and after co-culture, the cells removed by scraping were subcultured once every for 2-4 days. Strong cytotoxicity to a cell (C8 cells; a clone of F81 cell line which contains mouse sarcoma virus genome) was found in culture of cells from generations 11 after the passages of co-cultures. The activity to the cells was continuously found in the fluid without any other addition of stimulants. The above results show that J 774 cells were activated by the co-culture with BLV-FLK cells and produced the cytotoxic factor which revealed strong cytotoxicity to a C8 cells.

Animals↗
PubMed1986

The effect of Bacillus natto on the T and B lymphocytes from spleens of feeding chickens.

The effect of Bacillus natto on T and B lymphocytes in the chicken spleen was investigated. Bacillus natto is isolated from fermented food and is used in Japan in a preparation that enhances growth of farm animals. When chickens were fed 10(7) colony-forming units of B. natto (in spores) per gram of feed from hatching to 27 days of age there was an increase in the percentage of T and B lymphocytes in the spleens compared with non-B. natto-fed controls. The findings suggested that dietary Bacillus natto had an effect on cellular immune responses.

Animal Feed↗
PubMed1985

In vitro production of hydrogen peroxide by the amoebocytes of the scallop, Patinopecten yessoensis (Jay).

In vitro production of hydrogen peroxide (H2O2) by the amoebocytes of the scallop, Patinopecten yessoensis, was studied. The authors first confirmed the availability of the direct quantitative method using homovanillic acid, and found that both resting and stimulated amoebocytes produced H2O2. However, the latter showed higher capacity for H2O2 production. The amoebocytes stimulated by concanavalin A released a large amount of H2O2 compared with the cells challenged with three species of bacteria.

Animals↗
PubMed1984

Selective breeding for high and low antibody responses to inactivated Newcastle disease virus in Japanese quails.

Two lines of Japanese quail were divergently selected for high and low antibody responses after being twice injected at 4 and 6 weeks of age with inactivated Newcastle disease virus antigen. After seven generations of selection, the serum antibody level in the high line was 24% greater than the level in the unselected control lines, whereas the low line antibody level was approximately 37% less than that in the control line. The estimated heritability (H2S + D) was approximately .12 +/- .50. The realized h2, calculated from coefficient of regression formula on seven generations (G3 to G9 ), was .07.

Animals↗
PubMed1984

Immunological traits in generations 7 to 12 of two lines of Japanese quail selected for high or low antibody response to Newcastle disease virus.

Several immunological traits were compared in lines of Japanese quail selected for high and low secondary immune responses to anti-Newcastle disease virus (NDV). The high line of quail, selected for high ability to produce antibodies, was more responsive in both their primary response to NDV antigen used in the selection as well as to other antigens used in selection process including NDV (Ishi strain), tissue culture-derived Newcastle disease (TCND strain), NDV strain B1, influenza virus, sheep erythrocytes, and Salmonella pullorum than the low line, selected for low ability to produce antibody titers. Mitogenic response to phytohemagglutinin mitogen in the blood lymphocytes from high line quail was more reactive than those from low line quail. Significant line differences were also found in the number of spontaneous rosette cells produced by blood lymphocytes with both fowl and rabbit erythrocytes; the number in the high line exceeded those in the low line.

Animals↗
PubMedDate not supplied

The effect of Bacillus natto in feed on the sheep red blood cell antibody response in chickens.

Chickens fed 10(6) or 10(7) of Bacillus natto strain BN/g of diet from hatching through 15 or 30 days of age showed an increase in ability to produce hemagglutinating antibody titer as determined by intravenous injection of sheep red blood cells, whereas no increase in antibody production ability was observed in the chickens fed 10(7) of BN/g of diet from hatching through 10 days of age.

Agglutinins↗