PubMed Health⌕ Search

Biomedical subjects

S Issaragrisil

Publications and source records attributed to S Issaragrisil.

At least 55 records · Page 3Linked to original sources
PubMed1988

Methylprednisolone therapy in aplastic anaemia: correlation of in vitro tests and lymphocyte subsets with clinical response.

20 patients with aplastic anaemia were treated with methylprednisolone 1 g/d for 3 d followed by prednisolone 60 mg on alternate days. At 3 months after therapy, 7 of 20 patients (35%) showed recovery, 2 had died and 11 were non-responders. Only newly diagnosed patients were responders. Results of in vitro tests were correlated with clinical response. The first test was designed to determine whether removing various subpopulation of cells would increase the numbers of progenitor cells. In the second test blood cells were collected at 1 month after starting treatment and assayed for progenitor cells. Data from both tests did not correlate with the response to therapy. Lymphocyte subsets were measured in the blood before and at 1 month after treatment. The absolute numbers of OKT3+, OKT4+ and OKT8+ cells were significantly decreased compared to the normal population. The number of HNK1+ cells was also decreased but no significant difference was observed. Responders had a higher number of HNK1+ cells compared to non-responders. No significant differences of OKT3+, OKT4+ and OKT8+ cells among responders and non-responders were observed. Therefore a high number of HNK1+ cells prior to therapy may identify patients responding to the treatment.

Adolescent↗
PubMed1988

Studies on the pathogenesis of aplastic anemia in Thailand: evidence of immune-mediated mechanism.

The pathogenesis of aplastic anemia in Thailand was studied by using in vitro progenitor cells culture. In 37 patients who had active disease, the numbers of colonies derived from erythroid and granulocyte-macrophage progenitor cells (BFU-E and CFU-GM) were markedly decreased both in the blood and bone marrow as compared to normal controls. Co-culture of patients' cells with normal blood cells was performed in order to verify an immunologically mediated mechanism. In 8 of 26 patients, there were very low numbers of colonies both BFU-E and CFU-GM in the blood and bone marrow with significant suppression of colony formation in co-culture. Suppressor cells may have caused the aplasia in these patients. The rest had low colony formation and no suppression in co-culture. These patients may have absent or defective stem cells. None had normal colony formation. Therefore, aplastic anemia in Thailand may result mostly from defects involving the stem cells. Only some patients had cell mediated suppression of hematopoiesis as detected by co-culture.

Adolescent↗
PubMed1987

Corticosteroids therapy in paroxysmal nocturnal hemoglobinuria.

We evaluated the efficacy of alternate day, high dose prednisolone for the treatment of paroxysmal nocturnal hemoglobinuria (PNH). Nineteen patients were included. Thirteen were men and six were women, aged between 13-56 years. Eleven patients improved, eight with good response and three with fair response. Eight patients were non-responders. Responders had gradual improvement in the hemoglobin level, but none achieved a normal hemoglobin level. Age at diagnosis, sex, initial hemoglobin, white count, and percentage of a positive Ham's test had no apparent bearing on treatment outcome. A prolonged interval from diagnosis to prednisolone treatment decreased the chance of a favorable hematologic response to therapy. Age at the treatment in non-responders was higher than responders. Responders had higher numbers of colonies derived from BFU-E and CFU-GM both in the blood and bone marrow than non-responders although the differences did not achieve statistical significance. These data indicate that alternate day, high dose prednisolone therapy is effective in some patients with PNH.

Adolescent↗
PubMed1986

Methylprednisolone treatment in aplastic anaemia.

Twenty patients with aplastic anaemia were treated with a short term bolus of methylprednisolone. Seven patients were refractory to anabolic steroids and were observed in very long follow-up periods of 14-104 months. Thirteen patients had never been treated. The latter group also received anabolic steroids. Five out of 20 patients responded to the treatment while the remaining patients did not or died. Responders among patients refractory to anabolic steroids had shorter duration of disease compared to non-responders. No recurrence of aplasia was observed in all responders.

Adolescent↗
PubMed1986

Growth of mixed erythroid-granulocytic colonies in culture derived from bone marrow of patients with paroxysmal nocturnal hemoglobinuria without addition of exogenous stimulator.

Hematopoietic progenitor cell cultures were studied in 16 patients with paroxysmal nocturnal hemoglobinuria (PNH). Both erythroid and granulocyte-macrophage progenitors in the blood and bone marrow decreased despite hypercellular marrow. In eight out of 16 patients, mixed erythroid-granulocytic colonies were observed in the bone marrow cultures without erythropoietin and colony-stimulating factor. Removal of monocytes and T-lymphocytes before culture failed to abrogate spontaneous mixed-colony formation. Conditioned media prepared from blood and bone marrow of PNH patients could not promote the growth of mixed colonies in normal individuals. The autonomous, spontaneous mixed-colony formation in patients with PNH is an abnormality of PNH stem cells not described previously.

Antibody Formation↗
PubMed1984

Characterization of erythroid and granulocyte monocyte progenitors in human cord blood.

Some characteristics of both erythroid and granulocyte monocyte progenitors in human cord blood were compared to those in adult blood and bone marrow. The number of progenitors in cord blood was higher than that in adult blood and bone marrow. Most colonies in cord blood culture were monocyte-macrophage, whereas those from adult blood were largely eosinophilic. Cord blood progenitors had a slower sedimentation velocity than that reported for marrow, but sedimented faster than that for adult blood. A significant proportion of progenitors in cord blood as well as adult marrow was found to be in the DNA synthetic phase of the cell cycle whereas progenitors in adult blood were not. Cord blood BFU-E were more resistant than adult blood BFU-E but cord blood CFU-GM were not different from adult blood CFU-GM with regard to radiation sensitivity. Cord blood CFU-GM appeared to be more radio-resistant than adult marrow GFU-GM. From these results is seems clear that progenitors in cord blood differ in some aspects from those in adult blood and bone marrow.

Bone Marrow Cells↗
PubMed1983

Correlation between hematopoietic progenitors and erythroblasts in cord blood.

Erythroid and granulocyte-monocyte progenitors in cord blood were studied, using the methyl cellulose assay. The mean number of progenitors was 12-16 times higher than that of normal adult blood. The number of both erythroid and granulocyte-monocyte progenitors correlated positively with the number of erythroblasts present in cord blood. The possible origin of hematopoietic progenitors in cord blood was discussed.

Adult↗
PubMed1983

An association of pregnancy and autoimmune haemolytic anaemia.

14 cases of pregnancy-associated autoimmune haemolytic anaemia were analyzed. 4 cases were diagnosed during pregnancy; 10 cases became pregnant during the remission period of the disease. 6 cases were secondary to systemic lupus erythematosus. In the first group, the anaemia developed in the third trimester whereas in the second group the Hb fell slightly in the first trimester and rapidly between the third and fourth month of gestation. Only 1 case could withstand the hazard of pregnancy uneventfully; 4 needed corticosteroid and delivered normal infants; 2 developed toxaemia of pregnancy; in 5, either therapeutic or spontaneous abortion was noted and 1 death occurred due to renal complication. Abortion was induced in 1 case due to socio-economic indications.

Abortion, Induced↗
PubMed1983

Chronic myelocytic leukaemia: cytogenetical studies on haemopoietic colonies and diffusion chamber cultures.

In 20 patients with chronic myelocytic leukaemia in the chronic phase or in blast crisis, several in vitro (CFU-C, BFU-E) and in vivo (diffusion chamber) culture techniques were used to demonstrate the existence of a still diploid cell clone. In 4 out of 12 patients in the chronic phase, Ph1-negative metaphases were found in the various cultures for haemopoietic progenitor cells but not in the standard suspension cultures used for cytogenetics. However, all the 8 patients investigated during blast crisis had only Ph1-positive and other abnormal clones, indicating the loss of the diploid clone during progression of the disease.

Acute Disease↗