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S J Badger

Publications and source records attributed to S J Badger.

4 recordsLinked to original sources
PubMed1984

Cultures of thoracotomy specimens confirm usefulness of sputum cultures in cystic fibrosis.

Bacteriologic cultures of sputum and specimens obtained at thoracotomy from 17 patients with cystic fibrosis were compared. All organisms found in the surgical specimens were found in the sputum. Only two surgical specimens failed to yield any organisms. The most frequently isolated organisms were Pseudomonas aeruginosa (41%) and Pseudomonas cepacia (35%). Serotypes of P. aeruginosa and antibiogram results on both P. aeruginosa and P. cepacia further supported the concordance of the isolates from the two sources. In addition, anaerobic cultures were obtained in specimens from 10 patients with CF and revealed two patients with anaerobic colonization of the lower respiratory tract. These findings also correlated well with the sputum cultures. The correlation of sputum and lung specimen culture results supports the value of sputum culture in the management of lung disease in CF.

Adolescent↗
PubMed1983

A type-specific antigen of Eikenella corrodens is the major outer membrane protein.

Eikenella corrodens released antigenic material in the form of outer membrane fragments during growth. A culture filtrate antigen (AgA) from three strains reacted with antisera to whole bacteria with serotype specificity. A monoclonal antibody to the AgA of strain 1073 reacted with only 2 of 12 strains tested by enzyme-linked immunosorbent assay, immunofluorescence assay, and immunoelectrophoresis assay. The antigenic reactivity was resistant to boiling and to pronase and trypsin treatments. AgA was isolated from an outer membrane preparation after boiling in sodium dodecyl sulfate, radioiodinating the proteins, and precipitating AgA specifically with monoclonal antibody. A single protein band corresponding to the major outer membrane protein of E. corrodens was observed by autoradiography of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the immunoprecipitate. AgA had a molecular weight of 42,000, and its amino acid content resembled those reported for major outer membrane proteins with similar molecular weights from two other gram-negative organisms.

Amino Acids↗
PubMed1979

Experimental Eikenella corrodens endocarditis in rabbits.

The ability of Eikenella corrodens to cause endocarditis in catheterized rabbits was studied. E. corrodens 1073, the serum-resistant strain used in the study, was isolated from a human periodontitis lesion. Thirty-four rabbits, surgically catheterized across the aortic valve and injected intravenously 24 to 48 h later with 10(7) to 10(9) log-phase organisms, were studied. Only three rabbits developed positive blood cultures and only two rabbits died before the time of sacrifice at 14 days after infection. Autopsies showed that all rabbits developed aortic vegetations, 52% of which were culture positive for E. corrodens. The organisms were recovered from aortic vegetations in a mean concentration of 10(5.3) colony-forming units/g of tissue and from liver or kidney in 28% of the animals in concentrations from 10(2) to 10(4) colony-forming units/g. Indirect immunofluorescent staining of vegetations, with the use of specific rat antiserum to E. corrodens 1073 and fluorescein isothiocyanate-labeled goat antirat serum, revealed colonies of E. corrodens in culture-negative vegetations as well as in those which were culture positive. The results showed that E. corrodens was an effective pathogen in the rabbit model of endocarditis, in which the disease was infrequently bacteremic and rarely fatal.

Agglutinins↗