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Biomedical subjects

S J Guo

Publications and source records attributed to S J Guo.

6 recordsLinked to original sources

[Using L-form bacterium ATP bioluminescence assay for rapid testing L-form bacterial susceptibility].

Adenosine triphosphate is a kind of necessary metabolites in living cells. The authors detected ATP contents by using bioluminescence method in 111 strains of L-form bacteria after exposing to 5 kinds of antibiotics. The results showed that the mean value of CPM was less than (35 +/- 10.2)%.s-1. Thus, the value could be acted as a critical concentration between susceptibility and resistance. The conincidence rate of this method and K-B method was 95.3%. It indicates that the bioluminescence method has a high sensitivity. It can be used to detect L-form bacterium-drug susceptibility quickly and may play an important role for choosing the antibiotics.

Adenosine Triphosphate↗

Iridoids from Phlomis umbrosa.

Two epimeric pairs of iridoid aglycones, named shanzhigenin methyl ester and 1-epishanzhigenin methyl ester, and 8-acetylshanzhigenin methyl ester and 8-acetyl-1-epishanzhigenin methyl ester, were isolated from Phlomis umbrosa roots, along with five known iridoid glucosides. The four iridoid aglycones are reported for the first time from a natural source. Their structures were established by spectroscopic methods, mainly 1D- and 2D-NMR spectroscopic experiments, and chemical methods.

Acetylation↗

[Determination of lead in urine by differential potentiometric stripping analysis].

In the determination of lead in urine by differential potentiometric stripping analysis (DPSA) urine samples are acidified with hydrochloric acid to a total concentration equal to 0.2 mol/L without prior digestion with mineral acids. The sample is pre-electrolyzed at -1.2V (Vs SCE) for 80 seconds by glassy-carbon electrode coated with mercury. The lead concentrations are evaluated by means of standard addition. Detection limit of the method is 2ppb. The linear relationship occurs in the range of 0-150ppb. The relative standard deviations are 1.6% and the recovery of lead in urine samples is 89.5-110.0%. Compared with dithizone method, this method shows no significant difference in the results obtained.

Humans↗

Cochlear implant: our preliminary experience.

We have performed cochlear implants on 16 patients (12 cases with the plug type electrodes and 4 cases with the coil type electrodes) with analogue single channel electroacoustic stimulators. The follow-up period was 8 months to 3 years. The selection of patients, operative approach and the results of these cases are presented. Our present cochlear implant can bring the totally sensory deaf patient back to the world of sound but the sounds heard are distorted so they are unable to discriminate speech, although it improves their lipreading capacity and sense of safety. The results are encouraging but much more is to be desired and further research and investigation are required.

Adolescent↗